Adult trachea tissue was fixed using Karnovsky's fixative in 0.1 M sodium phosphate buffer (Sorenson's), washed with Sorenson's sodium phosphate buffer and post-fixed using 1% OsO4 in Sorenson's for 1 hour. Tissue was dehydrated by passaging through a graded series of ethanol solutions, then critical point dried using a Tousimis 931 Super Critical Point Dryer. The tissue was mounted on aluminum stubs and sputter coated with gold using a PELCO SC-7 coater. The samples were viewed on an FEI XL30 TMP SEM and digital images were collected. SEM was performed in the electron microscopy facility of the University of California at Davis.
Xl30 tmp sem
Manufactured by Thermo Fisher Scientific
The XL30 TMP SEM is a scanning electron microscope (SEM) that utilizes a turbomolecular pump (TMP) for vacuum generation. It is designed to provide high-resolution imaging capabilities for a wide range of samples. The core function of the XL30 TMP SEM is to generate and maintain a high-vacuum environment within the specimen chamber, allowing for the observation and analysis of samples at the nanoscale level.
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Lab products found in correlation
2 protocols using xl30 tmp sem
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Scanning Electron Microscopy of Trachea
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Scanning Electron Microscopy of Trachea
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Adult trachea tissue was fixed using Karnovsky's fixative in 0.1 M sodium phosphate buffer (Sorenson's), washed with Sorenson's sodium phosphate buffer and post-fixed using 1% OsO4 in Sorenson's for 1 hour. Tissue was dehydrated by passaging through a graded series of ethanol solutions, then critical point dried using a Tousimis 931 Super Critical Point Dryer. The tissue was mounted on aluminum stubs and sputter coated with gold using a PELCO SC-7 coater. The samples were viewed on an FEI XL30 TMP SEM and digital images were collected. SEM was performed in the electron microscopy facility of the University of California at Davis.
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