Drugs were dissolved in HBSS, which was used as the experimental vehicle, and applied directly to the media containing NGF. Drugs concentrations are based on published literature: choline (Acros Organics, Geel, Belgium) (10 mM, 3 mM, and 1 mM) [22 (link)]; α-bungarotoxin (Thermofisher) (BGTX) (50 nM) [32 (link)]; calpeptin (Sigma Aldrich, St. Louis MO, USA)(26 μM) [33 ], ryanodine (Santa Cruz) (30 μM) [21 (link)]; Xestospongin C. (Tocris Biosciences, Bristol, UK.) (Xest C.) (1 μM) [20 (link)]; FK506 (Tocris Biosciences) (40 μM) [34 (link)]; Substance P (Tocris Biosciences) (Sub P) (1 μM) [20 (link)]; PNU 282987 (Tocris Biosciences) (10 μM) [35 (link)].
Choline
Manufactured by Thermo Fisher Scientific
Sourced in Belgium
Choline is a nutrient that plays a crucial role in various biological processes. It is an essential component of cell membranes and is involved in the synthesis of the neurotransmitter acetylcholine, which is important for cognitive function and muscle control.
Automatically generated - may contain errors
Lab products found in correlation
Ryanodine, by Santa Cruz Biotechnology (1 mentions)
Calpeptin, by Merck Group (1 mentions)
α bungarotoxin, by Thermo Fisher Scientific (1 mentions)
Substance p, by Bio-Techne (1 mentions)
Fk506, by Bio-Techne (1 mentions)
Xestospongin c, by Bio-Techne (1 mentions)
Pnu282987, by Bio-Techne (1 mentions)
Levamisole, by Thermo Fisher Scientific (1 mentions)
Tyramine, by Merck Group (1 mentions)
Dopamine, by Merck Group (1 mentions)
Serotonin, by Merck Group (1 mentions)
Glutamate, by Merck Group (1 mentions)
Aspartate, by Merck Group (1 mentions)
D tubocurarine, by Merck Group (1 mentions)
Gabazine, by Merck Group (1 mentions)
Histamine, by Merck Group (1 mentions)
Acetylcholine, by Thermo Fisher Scientific (1 mentions)
Octopamine, by Merck Group (1 mentions)
Nicotine, by Thermo Fisher Scientific (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
2 protocols using choline
1
Neurotransmitter Regulation of NGF Signaling
2
Preparation of Neurotransmitter Stock Solutions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetylcholine (AC159170050, ACROS Organics), GABA (AC103280250, ACROS Organics), aspartate (11240, Sigma-Aldrich), ATP (A3377, Sigma-Aldrich), dopamine (H8502, Sigma-Aldrich), glutamate (49621, Sigma-Aldrich), octopamine (O0250, Sigma-Aldrich), serotonin (H9523, Sigma-Aldrich), tyramine (T2879, Sigma-Aldrich), nicotine (AC181420050, ACROS Organics), levamisole (AC187870100, ACROS Organics), choline (AC110295000, ACROS Organics), glycine (G46-1, Fisher Scientific), d-tubocurarine (T2397, Sigma-Aldrich), gabazine (S106, Sigma-Aldrich), and histamine (H7250, Sigma-Aldrich) were first dissolved in water to make frozen stock solutions (10 to 100 mM), which were diluted to final concentrations using extracellular solutions before use.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!