The raw materials, diets, digesta, and feces were ground using a laboratory mill (Arthur H. Thomas Co., Philadelphia, PA) to pass through a 0.5 mm mesh sieve. DM and nitrogen analysis methods, 934.01 and 976.05 [17 ], respectively, were performed. Chromium analysis was performed as described by Fenton and Fenton [18 ]. Gross energy was estimated using an adiabatic bomb calorimeter (model 1281, Parr, Moline, IL). Samples from the raw materials, diets, and digesta were hydrolyzed at 110°C for 24 h in 6 mol/L HCl to use in AA analysis method 994.12 [17 ]. For methionine and cystine analyses, oxidation with performic acid was carried out before acid hydrolysis [17 ]. Tryptophan was not estimated. AA analysis was performed using reverse phase HPLC (1100 HPLC Hewlett Packard), according to Henderson et al.[19 ]. Nitrogen in the liquid urine was estimated according to AOAC [17 ] method 976.05. Energy in the lyophilized urine was estimated according to Le Bellego [14 (link)].
1100 hplc
Manufactured by Hewlett-Packard
The 1100 HPLC is a high-performance liquid chromatography system designed for a wide range of analytical applications. It features precise solvent delivery, reliable autosampling, and sensitive detection capabilities.
Automatically generated - may contain errors
Lab products found in correlation
C18 reverse phase column, by Agilent Technologies (3 mentions)
Model 5415r, by Eppendorf (1 mentions)
Cary 100 bio uv vis spectrophotometer, by Agilent Technologies (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Cell culture media and supplements, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Tissue culture flasks and plates, by Corning (1 mentions)
Milli q system, by Merck Group (1 mentions)
Du 7400 spectrophotometer, by Beckman Coulter (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Oligonucleotide, by Integrated DNA Technologies (1 mentions)
9 protocols using 1100 hplc
1
Analytical Methods for Nutrient Evaluation
2
In Vitro OP Metabolism Kinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In vitro OP metabolism was studied by incubating different concentrations of OP solution (0.1-0.5 mg/ml) with 30 μl rat plasma and Tris-HCl buffer (pH 7.4) in a shaking water bath maintained at 37 °C. The final incubation volume was 200 μl. Control incubation was carried out in a similar manner but without rat plasma. At 1 h after incubation, ice-cold acetonitrile (400 μl) was used to terminate the hydrolysis reaction. The mixture was centrifuged at 13200 rpm in a 4 °C centrifuge (Model 5415R, Eppendorf AG, Barkhausenweg 122339 Hamburg, Germany) for 10 min to separate into layers. The supernatant was transferred to an HPLC vial and analyzed by a Hewlett Packard 1100 HPLC equipped with a UV detector. OP and OC were separated on an HPLC column (ODS-2, 150 mm, 5 μm) using gradient elution. The mobile phase consisted of a solution of 0.4 % phosphoric acid (pH 3.0) and acetonitrile (80:20, v/v). Acetonitrile in the mobile phase increased linearly from 20 % to 40 % in 10 min. The flow rate of the mobile phase was 1 ml/min. The detection wavelength was set at 215 nm. OP metabolism rate was expressed as μg OC formed/ml plasma/h. The Vmax and Km of OP metabolism were determined using the Lineweaver-Burke plot and were found to be 61.2 mg/h and 300 mg/L, respectively.
3
Synthesis and Characterization of Ruthenium(II) and Copper(II) Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals and starting materials were purchased
from commercial vendors and used as received. Ru(bpy)2Cl2 was prepared according to the literature.9 (link) UV–visible spectra were recorded on a
Beckman DU 7400 UV–visible spectrophotometer (Beckman Coulter)
from 200 to 800 nm. Oligonucleotides were synthesized using standard
phosphoramidite chemistry at Integrated DNA Technologies (Coralville,
IA) and purified by HPLC using a C18 reverse-phase column
(Varian, Inc.) on a Hewlett-Packard 1100 HPLC. The copper complex
Cu(phen)22+ was generated in situ by combining CuCl2 with phen ligands in a 1:3 ratio.
from commercial vendors and used as received. Ru(bpy)2Cl2 was prepared according to the literature.9 (link) UV–visible spectra were recorded on a
Beckman DU 7400 UV–visible spectrophotometer (Beckman Coulter)
from 200 to 800 nm. Oligonucleotides were synthesized using standard
phosphoramidite chemistry at Integrated DNA Technologies (Coralville,
IA) and purified by HPLC using a C18 reverse-phase column
(Varian, Inc.) on a Hewlett-Packard 1100 HPLC. The copper complex
Cu(phen)22+ was generated in situ by combining CuCl2 with phen ligands in a 1:3 ratio.
4
Oligonucleotide Synthesis and Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oligonucleotides
were synthesized using standard phosphoramidite chemistry at IDT DNA
(Coralville, IA) and purified by HPLC using a C18 reverse-phase
column (Varian, Inc.) on a Hewlett-Packard 1100 HPLC. Quantification
was performed on a Cary 100 Bio UV–vis spectrophotometer using
extinction coefficients at 260 nm (ε260) estimated
for single-stranded DNA.
were synthesized using standard phosphoramidite chemistry at IDT DNA
(Coralville, IA) and purified by HPLC using a C18 reverse-phase
column (Varian, Inc.) on a Hewlett-Packard 1100 HPLC. Quantification
was performed on a Cary 100 Bio UV–vis spectrophotometer using
extinction coefficients at 260 nm (ε260) estimated
for single-stranded DNA.
5
Quantitative Analysis of Retinoids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were quantified and cellular extracts were prepared as described [12 (link),23 (link)]. HPLC measurements were performed using a Hewlett–Packard 1100 HPLC equipped with a Zorbax Eclipse 5 μm XDB-C18 analytical column (250 X 4.6 mm; Agilent Technologies Inc, Palo Alto, CA). A linear gradient solvent system was used: 5% acetic acid aqueous solution/MeOH from 55:45 to 35:65 in 40 min; the flow rate was 1 mL/min. Peaks were detected by UV absorption (330 nm for retinol and 360 nm for the others) with a diode array detector. Acitretin was used as an internal standard. All standard reagents and solvents were purchased from Sigma-Aldrich (St. Louis, MO). LC-MS measurements were performed to double-check compounds on a Micromass/Waters LCT Premier Electrospray Time of Flight mass spectrometer coupled with a Waters HPLC system.
6
Carotenoid Profiling in Flower Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The petal extracts (3 mL) were filtered through a 0.45 µm membrane filter. The carotenoid extracts were analyzed using a Hewlett-Packard 1100 HPLC with diode array detector (DAD). The carotenoid analyses were carried out by using reversed phase C18 column Nucleosil ODS (250 x 4.6 mm), 5 µm. The mobile phase consisted of mixtures of acetonitrile: water (9:1, v/v) with 0.25 % triethylamine (A) and ethyl acetate with 0.25 % triethylamine (B). The gradient started with 90 % A at 0 min to 50 % A at 10 min. The percentage A decreased from 50 % at 10 min to 10 % of solvent A at 20 min. The flow rate was 1 ml/min and the chromatogram was monitored at 450 nm.
Carotenoids in the flower extracts were identified by their retention times in HPLC and by their UV/Visible absorption spectra compared to reference standards. Carotenoid standards were lutein, zeaxanthin, lycopene, ß-carotene, neoxanthin and violaxanthin. The purity of these standards was estimated by HPLC and was: 95 %-ß-carotene, 98.5 %-lutein, 97.7 %-lutein and neoxanthin and violaxanthin. These standards were purchased from Sigma Aldrich.
Carotenoids in the flower extracts were identified by their retention times in HPLC and by their UV/Visible absorption spectra compared to reference standards. Carotenoid standards were lutein, zeaxanthin, lycopene, ß-carotene, neoxanthin and violaxanthin. The purity of these standards was estimated by HPLC and was: 95 %-ß-carotene, 98.5 %-lutein, 97.7 %-lutein and neoxanthin and violaxanthin. These standards were purchased from Sigma Aldrich.
7
Comprehensive Gallstone Composition Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Assessment of the composition of gallstones (total cholesterol, bile acids, calcium ions and bile pigments) with a powder deposition mass was performed in accordance with the method described by Steen and Blijenberg [20 (link)]. Total cholesterol was determined by Color Test – Cholesterol Oxidase/Peroxidase (BioSystems). Determination of total bile acids was performed using the enzyme assay – Merckotest Bile Acids (Merck). The content of bilirubin was determined by spectrophotometry using a Bilirubin Diazotized Sulfanilic-Color Kit (BioSystems). The composition of the fatty acids was evaluated by liquid chromatograph Hewlett Packard 1100 HPLC.
The concentration of cholesterol and bile acids in the tested samples was calculated in relation to the reference test in accordance with the Beer-Lambert law. The obtained cholesterol values (mmol/cm3), bile acids (µmol/cm3) and bilirubin (pmol/dm3) were converted to the amount of these compounds in the analyzed samples of gallstones (mg/100 mg of deposit). Calcium carbonate content was determined according to the method described by Scheibler. The volume of evolved CO2 was converted to standard conditions, then the amount of calcium carbonate was expressed as mg/100 mg of deposit.
The concentration of cholesterol and bile acids in the tested samples was calculated in relation to the reference test in accordance with the Beer-Lambert law. The obtained cholesterol values (mmol/cm3), bile acids (µmol/cm3) and bilirubin (pmol/dm3) were converted to the amount of these compounds in the analyzed samples of gallstones (mg/100 mg of deposit). Calcium carbonate content was determined according to the method described by Scheibler. The volume of evolved CO2 was converted to standard conditions, then the amount of calcium carbonate was expressed as mg/100 mg of deposit.
8
Synthesis and Characterization of Rhodium Metal Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals, reagents, and solvents used for synthesis were commercially available, unless otherwise noted, and used as received. Organic solvents were purchased from Sigma-Aldrich unless otherwise noted. Water was purified using the Millipore Milli-Q system. [Rh(chrysi)(phen)(2-(pyridine-2-yl)propan-2-ol]Cl2 (Rh-PPO) and [Rh(chrysi)(phen)(1-Phenyl-1-(pyridine-2-yl)ethan-1-ol.]Cl2 (Rh-PPE) were synthesized following published methodology (22 (link)). Oxaliplatin was purchased from Alfa Aesar. High-performance LC (HPLC)–grade acetonitrile and methanol were purchased from Fisher Scientific. Formic acid (99% pure) was purchased from Acros Organic. Sep-Pak C18 solid-phase extraction cartridges were acquired from Waters Chemical Co. All HPLC metal complex purifications were carried out on a Hewlett-Packard 1100 HPLC. All ultraviolet-visible (UV-Vis) spectroscopic experiments were performed on a Cary 100 spectrometer. Cell culture media and supplements were purchased from Life Technologies. Cell lines used in the experiment were purchased from ATCC. Tissue culture flasks and plates were obtained from Corning.
9
Oligonucleotide Synthesis and Purification
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!