The acid mine wastewater was sampled from iron ore processing plant of Xiangtan City in Hunan Province, China. In order to analyze the application potential of the bioflocculant in acid mine waste water treatment, the biofluccolant was added into the acid mine wastewater at the final concentration of 25 mg/L without pH adjustment. The suspension was shaken at 200 rpm and 30°C for 30 min. Then the flocculating activity was calculated according to the change of turbidity based on the absorbance at 550 nm and the pH change was record by a pH meter ((FiveEasy Plus pH, Mettler, United States). The supernatant was collected for detecting the concentration of Fe, Al, Mn, Cu, Pb, Zn, Ni and Cd by inductively coupled plasma atomic emission spectroscopy (ICP-AES, Hitachi Limited., Japan). The metal removal rate was calculated as follows: where C0 and C were the initial and final concentrations of the metal, respectively.
Icp aes
Manufactured by Hitachi
Sourced in Japan
The ICP-AES (Inductively Coupled Plasma Atomic Emission Spectrometry) is a laboratory equipment used for the elemental analysis of various samples. It utilizes an inductively coupled plasma to atomize and ionize the sample, and then measures the specific wavelengths of light emitted by the excited atoms to identify and quantify the elements present in the sample.
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Lab products found in correlation
Tissue culture dish, by BD (1 mentions)
1 n nitric acid solution, by Fujifilm (1 mentions)
100 mm dishes, by BD (1 mentions)
Imiquimod, by InvivoGen (1 mentions)
Protein assay, by Bio-Rad (1 mentions)
V 550, by Jasco (1 mentions)
Rint 2500, by Rigaku (1 mentions)
Tristar 2, by Micromeritics (1 mentions)
5 protocols using icp aes
1
Bioflocculant for Acid Mine Wastewater Treatment
2
Boron Uptake in Rat Glioma Cells
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The intracellular 10B concentrations were measured by means of inductively coupled plasma atomic emission spectroscopy (ICP-AES). For the in vitro boron uptake studies, F98 rat glioma cells were used. First, 105 F98 glioma cells were seeded into a tissue culture dish (100 × 20 mm; Becton Dickinson, Franklin Lakes, NJ) with culture medium (described above) at 37 °C in a 5% CO2 atm. After incubation for 5 days at 37 °C, the medium was replaced with culture medium (described above) containing 1 mM of ACBC-BSH, BSH, or BPA, and the cells were incubated for an additional 24 h at 37 °C. The medium then was removed, and the cells were washed twice with phosphate-buffered saline (PBS) and detached with trypsin-ethylenediamine tetraacetic acid solution. PBS was then added, and the cells were centrifuged twice and counted and sedimented.
The cells were then digested for 1 week with 1 N nitric acid solution (Wako Pure Chemical Industries, Osaka, Japan), and the boron uptake was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES: Hitachi, Tokyo). Pairwise comparisons were conducted using Student’s t-test. Group differences resulting in p-values < 0.05 were considered significant.
The cells were then digested for 1 week with 1 N nitric acid solution (Wako Pure Chemical Industries, Osaka, Japan), and the boron uptake was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES: Hitachi, Tokyo). Pairwise comparisons were conducted using Student’s t-test. Group differences resulting in p-values < 0.05 were considered significant.
3
BPA Exposure and Boron Uptake
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In this study, we examined whether exposure to BPA resulted in boron uptake into the human lymphoma cells, Raji and RL. First, 1 × 105 Raji and RL cells/mL were seeded in 100-mm dishes (Becton, Dickinson, and Company, Franklin Lakes, NJ, USA) at 37 °C in a 5% CO2 atmosphere using the culture medium described above. After 1 day of incubation at 37 °C, the medium was replaced with a culture medium containing 5, 10, and 20 μg B/mL of BPA, followed by incubation at 37 °C for 3 h. The medium containing BPA was removed, and the dishes were washed twice with phosphate-buffered saline (PBS). Then, PBS was added and centrifuged twice to count and sediment the cells. The cells were then lysed overnight in a 1N nitric acid solution. The boron concentration in the cells was measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES; Hitachi, Tokyo, Japan).
4
Ovalbumin and TLR7a Loaded Gadolinium Nanotubes
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Chicken egg ovalbumin (OVA, Sigma-Aldrich, St. Louis, MO, USA, 1 mg/mL in saline) and TLR7a (imiquimod, InVivoGen, San Diego, CA, USA, 0.2 mg/mL in saline) were mixed with Gd2O3 nanotubes (10 mg/mL) at 4 °C for 1 day. The supernatants were collected by centrifugation. The remaining OVA in the supernatant was tested by Bio-Rad Protein Assay (Bio-Rad Laboratories, Inc., Hercules, CA, USA). The remaining TLR7a in the supernatant was tested by an ultraviolet–visible spectrophotometer (V-550, JASCO, Tokyo, Japan). The loading efficiencies of OVA and TLR7a were calculated by the following formula: loading efficiency = (Initial biomolecule concentration−Biomolecule concentration after loading)/Initial biomolecule concentration × 100%.
To examine the biomolecule release and Gd2O3 nanotube degradation, the biomolecule-loaded Gd2O3 nanotubes (2.5 mg) were added to the acetate buffer (2 mL, pH = 5) or the Tris-HCl buffer (2 mL, pH = 7.4) at 37 °C. At certain time intervals, the buffers were collected, and at the same time, 1 mL of fresh buffers was added. The collected buffers were analyzed for OVA and TLR7a concentrations. The Gd ion concentrations in the collected buffers were analyzed by inductively coupled plasma-atomic emission spectrometry (ICP-AES, Hitachi High-Technologies, Ibaraki, Japan).
To examine the biomolecule release and Gd2O3 nanotube degradation, the biomolecule-loaded Gd2O3 nanotubes (2.5 mg) were added to the acetate buffer (2 mL, pH = 5) or the Tris-HCl buffer (2 mL, pH = 7.4) at 37 °C. At certain time intervals, the buffers were collected, and at the same time, 1 mL of fresh buffers was added. The collected buffers were analyzed for OVA and TLR7a concentrations. The Gd ion concentrations in the collected buffers were analyzed by inductively coupled plasma-atomic emission spectrometry (ICP-AES, Hitachi High-Technologies, Ibaraki, Japan).
5
Comprehensive Characterization of MS-Cu Nanospheres
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The MS-Cu nanospheres were characterized using a transmission electron microscope (TEM, JEOL, Akishima, Japan) and a powder X-ray diffractometer with CuKα X-rays (RINT 2500, Rigaku, Tokyo, Japan). The nitrogen gas (N2) adsorption–desorption isotherm of the MS-Cu nanospheres was measured using a surface area and porosity analyzer (TriStar II, Micromeritics). The BET specific surface areas and pore size distributions were calculated. The Cu/Si molar ratios of the MS-Cu nanospheres were examined by dissolving the nanospheres in 1M NaOH and 2M HCl, followed by inductively coupled plasma–atomic emission spectrometry (ICP-AES, Hitachi High-Technologies, Tokyo, Japan). In vitro copper ion release was studied by immersing nanospheres (1 mg/mL) in an acetate buffer (pH = 5) at room temperature. At certain time intervals, the supernatants were collected, and new buffers were supplemented. The copper ion release was analyzed by ICP-AES. The stability of PEG-MS-Cu nanospheres was tested by performing dynamic light scattering analysis (Otsuka Electronics, Osaka, Japan).
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