Gsh px test kit

GSH-px was obtained according to the GSH-px test kit protocol of (Nanjing Jiancheng, Nanjing, China). Briefly, an appropriate amount of liver tissue was grinded with saline to obtain the supernatant extract. A blank tube, standard tube, enzyme tube, and non-enzyme tube were set up. The reagents were mixed together according to the recommended quantities and the absorbance was measured at a wavelength of 412 nm.

Triglyceride levels were measured using the Triglyceride Assay Kit (A110-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer’s instructions. Liver tissues were accurately weighed and homogenized in ice water bath conditions with saline at a weight–volume ratio of 1 g: 9 mL. The supernatant was collected after centrifugation at 2500 rpm for 10 min for measurement. Liver antioxidant indexes were measured using commercial kits from Nanjing Jiancheng Bioengineering Institute and according to their instructions: the total superoxide dismutase (SOD) test kit (A001-1), malondialdehyde (MDA) test kit (A003-1), catalase assay (CAT) test kit (A007-2), and glutathione peroxidase (GSH-Px) test kit (A005-1).

With a GSH-PX test kit (Nanjing Jiancheng Bioengineering Institute, A005), the enzyme activity can be calculated according to the consumption of reduced glutathione in the enzymatic reaction. Cell protein was extracted according to the above method, and BCA assays were used to measure the protein concentration of GSH-PX. First, the optimal sampling concentration was determined, and an inhibition rate of 45% to 50% was taken as the optimal sampling concentration. The inhibition rate formula was as follows: inhibition rate = (nonenzyme tube-enzyme tube) OD value/nonenzyme tube-enzyme tube OD value × 100%, and the result was between 15 and 55%. Then, the OD value of each tube was measured at a wavelength of 412 nm according to the instructions, and the GSH-PX activity was calculated.