Mouse anti sox9

H&E histological staining was performed on 7μm-thick sagittal sections (for 12.5 dpc, 14.5 dpc and 6 weeks) according to standard protocols. For the experiments on the TESCO deletion, immunofluorescence staining was also performed on 7μm-thick sagittal sections (for TESCO) or 12 μm-thick sections (for TES), as described elsewhere [53 (link)], using (as primary and secondary antibodies, respectively): goat anti-AMH (1:500, Santa Cruz Biotechnology) and donkey anti-goat Alexa Fluor 488 (1:200, Invitrogen); mouse anti-SOX9 (1:100, Abnova) and donkey anti-mouse Alexa Fluor 647 (1:200, Invitrogen); rabbit anti-FOXL2 (1:650, generated as described by [53 (link)] and donkey anti-rabbit Alexa Fluor 647 (1:200, Invitrogen). For the experiments on the TES deletion, immunofluorescence staining was performed using rabbit anti-SOX9 (1:300, a generous gift from Francis Poulat, Institute of Human Genetics, Montpellier, France) with donkey anti-rabbit Alexa Fluor 488 (1:500, Invitrogen) and goat anti-Foxl2 (1:300, Novus) with donkey anti-goat Alexa Fluor 568 (1:500, Invitrogen). All immunofluorescence slides were also stained with 4',6-diamidino-2-phenylindole (DAPI, Molecular Probes), to visualize nuclear DNA.