E cadherin and n cadherin antibodies

SuperScript™III, RPMI 1640 medium and Opti-MEM medium were obtained from Invitrogen (Carlsbad, CA). TriSolution Plus Reagent was from GeneMark (Atlanta, Georgia). RNAzol^® RT RNA isolation reagent was purchased from Molecular Research Center, INC (Cincinnati, OH). GoScript^TM Reverse Transcriptase, Go Taq^® Green Master Mix (2X), and luciferase assay system were from Promaga (Madison, WI). G418 disulfate salt was from Sigma-Aldrich (St. Louis, MO). Src inhibitor PP1 was from Calbiochem (Merck Millipore, Darmstadt, Germany). Src inhibitor-1 was from Merck Millipore (Burlington, MA, USA). HyFect^TM DNA transfection reagent was from Leadgene Biomedical (Tainan, Taiwan). Antibodies against β-actin and Flag were obtained from Sigma-Aldrich (St. Louis, MO). E-cadherin and N-cadherin antibodies were obtained from BD Bioscience (Bedford, MA). MMP-2 antibody was purchased from Millipore (Billerica, MA). Antibodies against CDCP1, c-Src, pSrc^Tyr416, and pPKCδ^Tyr314 were purchased from Cell Signaling Technology (Danvers, MA).

Recombinant human VEGFA (R&D, Cat. no. 293-VE) was used for western blotting and live cell imaging experiments to stimulate VEGF signalling. Fibronectin antibody was used for western blotting (1:2,000) and immunofluorescence imaging (1:1,000) (Abcam, Cat. no. ab2413). Notch1 antibody (1:1,000) and VEGF-neutralizing antibody (1:1,000) were obtained from R&D Systems (Cat. nos. AF3647 and MAB293). Primary antibodies against Dll4 (1:500) and VEGFA (1:500) were obtained from Santa Cruz (Cat. nos. sc-365429 and sc-152). VE-cadherin (1:500), GAPDH (1:20,000) and phospho-FAK^Y397 (1:500) primary antibodies were obtained from Cell Signaling (Cat. nos. 2158, 2118 and 8556). Vimentin antibody was from Sigma (V6630, 1:1,000). E-cadherin and N-cadherin antibodies (1:1,000) were obtained from BD Biosciences (Cat. nos. 610181 and 610920). Alexa Fluor 555 and 647 secondary antibodies (Invitrogen) and DAPI (4′,6-diamidino-2-phenylindole; Invitrogen) were used for 3D immunofluorescence. Horseradish peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch) were used for western blotting.