Aia 2000 automated immunoassay analyzer

The serum levels of cardiac troponin I (cTNI) and myoglobin (Myo) were determined with chemiluminescence by the Tosoh Bioscience AIA-2000 Automated Immunoassay Analyzer using commercial reagents (Tosoh Corporation, Tokyo, Japan). Creatine kinase (CK), creatine isoenzyme (CK-MB), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were measured on a 7600 Hitachi Automatic Analyzer with commercial reagents (Hitachi High-Technologies Corporation, Tokyo, Japan). Interleukin-6 (IL-6) was studied with Roche diagnostic kits in the Cobas e-411 Roche Auto Analyzer. Serum IMA was analyzed using principle of albumin-cobalt binding defined by Nazik et al. (Nazik et al., 2017 (link)) on the Mindray BS-2000M Auto Analyzer (Mindray, Shenzhen, China) and C-reactive protein (CRP) was measured using the Mindray BC-5390 Auto Hematology Analyzer (Mindray, Shenzhen, China).

Peripheral blood samples of participants were drawn into vacutainers containing dipotassium ethylenediaminetetraacetic acid (K2EDTA) (BD Vacutainer^®, Becton Dickinson UK Ltd., Wokingham, UK). For all routine clinical chemistry assays, samples were directly transported to the laboratory of our hospital. XE-5000 (Sysmex, Kobe, Japan) and UniCel^® DxH 800 Coulter^® Cellular Analysis System automated hematology analyzer (Beckman Coulter, Miami, FL, USA, JAV) was used for the complete blood count test. An AIA-2000 automated immunoassay analyzer (Tosoh Bioscience, South San Francisco, CA, USA, JAV) was used for the immunoglobulin E (Ig E) level test. Blood tests were processed under tightly controlled and monitored conditions. A detailed blood analysis is described below.