Blood from each animal was collected in a stopper tube (without anticoagulants) and centrifuged: serum samples were then immediately frozen for further biochemical analyses. Abbott specific kits for automated chemistry analyzer (Architect C8000 Plus, Abbott, Sesto San Giovanni (MI) Italy) were used for the evaluation of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine, glucose, triglycerides, urea, total cholesterol, high density lipoprotein (HDL), C-reactive protein (PCR), creatine kinase MB (CK–MB) and lactate dehydrogenase (LDH), according to the manufacturer’s instructions.
Architect plus c8000
Manufactured by Abbott
Sourced in United States
The Architect Plus C8000 is a fully automated, high-throughput clinical chemistry analyzer developed by Abbott. It is designed to perform a wide range of clinical tests, including but not limited to chemical, immunoassay, and enzyme assays. The Architect Plus C8000 is capable of processing a large volume of samples with high accuracy and efficiency.
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Lab products found in correlation
8 protocols using architect plus c8000
1
Serum Biochemical Analysis Protocol
2
Cytotoxicity Evaluation via LDH and CK-MB
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Tissue damage was evaluated by measuring two cytotoxic markers, lactate dehydrogenase (LDH) (Abbott Laboratories, Abbott Park, IL, USA, 2P56-21) and creatine kinase MB isozyme (CK-MB) (Abbott, 6K25-30), in blood samples according to the manufacturer's instructions with the aid of a clinical chemistry analyzer (Abbott, Architect C8000 Plus).
3
Assessing Ischemic Albumin Biomarker
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IMA levels were determined by albumin cobalt binding test, a rapid colorimetric method developed by Bar-Or et al. [16 (link)]. The method is based on the binding ability of reduced cobalt ions (Co2+) of HSA due to ischemia. Briefly, a known amount of exogenous Co (CoCl2) was added to serum samples. Albumin, which is altered as a result of ischemic processes, binds to the Co(II) to a far lesser extent and the excess (unbound) amount of Co2+ forms a colored complex with dithiothreitol, which is measured spectrophotometrically at 480 nm. Plasma albumin levels were measured in a calibrated and well-controlled autoanalyzer using the bromocresol green method (Architect Plus C8000, Abbott, USA).
4
Diagnosing Dysglycemia Using OGTT
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Participants underwent a 75-gram OGTT after an overnight fast at recruitment. Venous fasting plasma glucose (FPG), 2-hour plasma glucose (2hPG) and HbA1c were measured (Abbott Architect Plus c8000, Wiesbaden, Germany). Pre-diabetes is defined as impaired fasting glucose (IFG): FPG 6.1–6.9 mmol/L with 2hPG < 11.1 mmol/L, or impaired glucose tolerance (IGT): FPG < 6.1 mmol/L with 2hPG ≥ 7.8 and <11.1 mmol/L. Diabetes is defined as: FPG ≥ 7.0 mmol/L or 2hPG ≥ 11.1 mmol/L9 . Participants with IFG, IGT or diabetes were collectively grouped as dysglycemia.
5
Spectrophotometric Analysis of Samples
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All reagents and chemicals were purchased from Sigma-Aldrich. Spectrophotometric analysis was performed with a Shimadzu UV-1800 spectrophotometer with a temperature-controlled cuvette holder and an automated analyzer (Architect Plus C8000, Abbott, USA).
6
Biochemical Indicators Measurement Protocol
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Biochemical indicators were detected by an automatic biochemical analyzer (Architect Plus C8000, Abbott, USA) in clinical laboratory of our hospital, including triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C).
7
Serum IMA Levels in Cerebral Infarction
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Peripheral venous blood samples were collected from patients and healthy control into tubes with EDTA, immediately after diagnosed for CI. Samples were centrifuged at 3500 rpm/min for 15 min at room temperature. The plasma samples were extracted and frozen below -80°C for use. A Albumin cobalt assay (ACB) was used to determine the serum IMA levels as described in a previous study (13) (link). ACB Assay for IMA was done colorimetrically as follows: 6200 μL of serum was mixed with 50 μl of CoCl2 (1 g/L) vigorously for 10 min, 50 μL of dithiothreitol (DTT) (1.5 g/L) was added and left for 2 min, and 0.9 N NaCl was then added. Plasma albumin levels were measured in a calibrated and well-controlled autoanalyzer using the bromocresol green method (Architect Plus C8000, Abbott, USA). It was read colorimetrically at 470 nm as ABS U/mL (absorbance units). The conditions of patients after treatment were assessed by National Institutes of Health Stroke Scale (NIHSS). The correlation between serum IMA levels at admission and NIHSS after treatment was also analyzed.
8
Ischemia-Modified Albumin Measurement Protocol
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We measured Ischemia-Modified Albumin (IMA) levels by using cobalt binding test developed by Bar-Or et al. [13] . Briefly, this method is based on the binding of cobalt with dithiothreitol (DTT) and measuring spectrophotometrically at 480 nm by adding a known amount of cobalt into the serum. The results were expressed as absorbance units (ABSU) (Abbot Architect C-8000). Plasma albumin levels were measured in autoanalyzer by using bromecresol green method (Architect Plus, C-8000, Abbott, USA).
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