Hepg2

Manufactured by Cobioer Biosciences

Sourced in China

The HepG2 is a type of cell line derived from human hepatocellular carcinoma. It is commonly used as an in vitro model for liver-related studies.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Mcf 7, by Cobioer Biosciences (1 mentions) Bronchial epithelial growth medium (begm), by Lonza (1 mentions) Thle 3, by American Type Culture Collection (1 mentions) Snu 449, by Cobioer Biosciences (1 mentions) N 1 naphthyl ethylenediamine dihydrochloride, by Merck Group (1 mentions) Sinapic acid, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Hyclone, by Cytiva (1 mentions) Penicillin streptomycin solution, by Cytiva (1 mentions) Hep3b, by Cobioer Biosciences (1 mentions) Dmem medium, by Corning (1 mentions) Hepg2, by Corning (1 mentions) Cc 3170, by Lonza (1 mentions) Bel7402, by Cobioer Biosciences (1 mentions) Mhcc97l, by Cobioer Biosciences (1 mentions)

8 protocols using hepg2

Transfection and Combinatory Treatment of HCC Cells

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Human HCC cell lines Huh7 and HepG2 were obtained from Nanjing Cobioer Biosciences (Nanjing, China). Both cells were cultured in Dulbecco’s modified Eagle’s medium (Cat#11995065, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Cat#10099141C, Gibco, Australia) and 1% Penicillin Streptomycin (Cat#15140122, Gibco), cultured at 37°C in a 5% CO₂ environment.
Duplex RNAs were transfected into cells using Lipofectamine^™ RNAiMAX (Cat#13778075, Invitrogen, USA) by following the reverse transfection method provided in the manufacturer’s manual for 72 hours unless otherwise stated.
For combinatory treatment with chemical compounds, HepG2 cells were plated in 96-well plates and reverse transfected with duplex RNAs for 24 hours, and then compounds (ssorafenib 1 μM, lenvatinib 5 μM, regorafenib 1 μM, or cabozantinib 5 μM) or DMSO control (0.1%) was added directly to the medium and the cells were further cultured for 48 hours before analysis.

Bi C.Q., Kang T., Qian Y.K., Kang M., Zeng X.H, & Li L.C. (2024). Upregulation of LHPP by saRNA inhibited hepatocellular cancer cell proliferation and xenograft tumor growth. PLOS ONE, 19(5), e0299522.

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Cell Cytotoxicity Assay Protocol

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All the cell lines used in this study were either purchased from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (A549, HepG2, MCF-7, PC-3 and LO2) or from Nanjing Cobioer Biosciences Co., Ltd. (Karpas299). Cells were incubated with 5% CO₂ at 37 °C for 24 h. Subsequently the compounds and the reference were dissolved into the culture medium. Then, the cells were treated with different concentrations of test compounds and further incubated. After drug treatment, 20 μL of MTT solution at 5 mg mL⁻¹ was added and incubated for 4 h. 100 μL of DMSO was added into each well to dissolve the purple formazan formed. The absorbance was determined at 630 nm using a plate reader. The IC₅₀ was calculated using GraphPad Prism version 6.0 software (San Diego, USA) from the non-linear curve.

Cai D., Zhang Z.H., Chen Y., Ruan C., Li S.Q., Chen S.Q, & Chen L.S. (2020). Design, synthesis and biological evaluation of novel amide-linked 18β-glycyrrhetinic acid derivatives as novel ALK inhibitors. RSC Advances, 10(20), 11694-11706.

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Culturing Diverse Hepatocellular Carcinoma Cells

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HCC cells including HLF, Huh-7, SNU-449, HepG2 and LM3 were obtained from COBIOER (Nanjing, China). HLF, Huh-7 and LM3 cells were grown in DMEM. SNU-449 cells were grown in RPMI-1640 medium. HepG2 cells were grown in MEM. All mediums were obtained from Gibco (Grand Island, USA). Human liver epithelial cell (THLE-3) was obtained from ATCC (Manassas, VA, USA) and kept in BEGM (Lonza/Clonetics Corporation, Walkersville). Cells were left to grow at 37 °C under a humid environment with 5% CO₂.

Zhang Z., Zhou Y., Jia Y., Wang C., Zhang M, & Xu Z. (2022). PRR34-AS1 promotes exosome secretion of VEGF and TGF-β via recruiting DDX3X to stabilize Rab27a mRNA in hepatocellular carcinoma. Journal of Translational Medicine, 20, 491.

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Purification and Characterization of Bioactive Compounds

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BFL and CBF with a purity over 98% were purchased from Chengdu Must Biotech Co., Ltd. (Chengdu, China). Cisplatin were purchased from Solarbio Biosciences Company (Beijing, China). Sinapic acid (SA), α-cyano-4-hydroxycinnamic acid (HCCA), 2,5-dihydroxybenzoic acid (DHB), peptides and N-(1-naphthyl)-ethylenediamine dihydrochloride (NEDC) were purchased from Sigma-Aldrich (MO). Phosphate-buffered saline (PBS) was purchased from GIBCO (Grand Island, NY). Human hepatoma cell line HepG2 was purchased from Cobioer Biosciences Company (Nanjing, China).

Zhang J., Hong Y., Xie P., Chen Y., Jiang L., Yang Z., Cao G., Chen Z., Liu X., Chen Y., Wu Y, & Cai Z. (2021). Spatial Lipidomics Reveals Anticancer Mechanisms of Bufalin in Combination with Cinobufagin in Tumor-Bearing Mice. Frontiers in Pharmacology, 11, 593815.

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Evaluating Immunotherapy Potential in Liver Cancer

Cited 2 times

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Immunotherapy has been a powerful method for cancer treatment [27 ]. Therefore, the potential clinical effects of immunotherapy on the two risk groups of patients were determined by The Tumor Immune Dysfunction and Exclusion (TIDE) algorithm (http://tide.dfci.harvard.edu/query/) [28 (link)]. Wherein, the higher TIDE score, the more possibility for immune escape. In the opposite, the lower TIDE score, the more benefit from immunotherapy. And we further analyzed the differences in immune checkpoint blockade (ICB) key molecules (KRAS and EGFR) [29 (link),30 ] between the different risk subgroups. Moreover, the relationship between different risk groups and the expressions of 4 major mismatch repair genes (MLH1, PMS2, MSH6, and MSH2) was analyzed.
Human liver cancer cell lines HepG2 and normal human liver cell lines LO2 were commercially purchased from COBIOER (Nanjing, China). Cells was maintained in RPMI medium containing 10 % FBS, 1 % penicillin/streptomycin (Invitrogen, Grand Island, NY). The negative control (si NC) and BACE1_AS siRNA (Sagon, China) were transfected into the cells by Lipofectamine 2000 (Invitrogen, USA) for 48 h.

Yue Y., Tao J., An D, & Shi L. (2024). A prognostic exosome-related long non-coding RNAs risk model related to the immune microenvironment and therapeutic responses for patients with liver hepatocellular carcinoma. Heliyon, 10(2), e24462.

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Culturing Hepatocellular Carcinoma Cell Lines

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HepG2 (CoBioer Biosciences Co., Ltd.; cat. no. CBP60199), Hep3B (CoBioer Biosciences Co., Ltd.; cat. no. CBP60197), PLC (CoBioer Biosciences Co., Ltd.; cat. no. CBP60223), Huh7 (CoBioer Biosciences Co., Ltd.; cat. no. CBP60202) and MIHA (Hunan Fenghui Biotechnology Co., Ltd.; cat. no. CL0469) cells were cultured in DMEM (cat. no. 12430104; Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (cat. no. 10091148; HyClone; Cytiva) and 1% penicillin-streptomycin solution (cat. no. 15140163; Gibco; Thermo Fisher Scientific, Inc.; ratio 9:1:1) at 37°C and 5% CO₂. Cross-contamination of the cell lines was excluded by short tandem repeat profiling (17 (link),18 (link)).

Sun G., Yang L., Wei S., Jin H., Li B, & Li H. (2021). miR-425 regulates lipophagy via SIRT1 to promote sorafenib resistance in liver cancer. Oncology Letters, 22(4), 695.

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PAFAH1B3 Silencing in Liver Cancer

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The LO2 cell line was purchased from the cell bank of Kunming Institute of Zoology, and cultured in DMEM media (Lonza, CC-3170). Liver cancer cell lines, including HepG2, Hu7, and SMCC-7721, were purchased from Cobioer, China, with STR document, HepG2, Hu7, and SMCC-7721 cells were all cultured in DMEM medium (Corning) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. The siRNA for PAFAH1B3 were synthesized by RIBOBIO, and a scrambled siRNA was synthesized as a negative control. Transfection was performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. Total RNA was collected 48 h after transfection.

Yuan Y., Jiang X., Tang L., Wang J, & Duan L. (2022). Comprehensive Analysis of the Prognostic and Immunological Role of PAFAH1B in Pan-Cancer. Frontiers in Molecular Biosciences, 8, 799497.

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Cultivating Liver Cancer Cell Lines

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Six liver cancer cell lines (HepG2, PLC/PRF/5, MHCC-97H, MHCC-97L, Bel-7402, and SK-Hep-1) were purchased from NANJING COBIOER BIOSCIENCES CO., LTD and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. Cells in logarithmic growth phase were applied to experiments.

Tian K., Wei J., Wang R., Wei M., Hou F, & Wu L. (2023). Sophoridine derivative 6j inhibits liver cancer cell proliferation via ATF3 mediated ferroptosis. Cell Death Discovery, 9, 296.

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