BD DifcoTM skim milk was purchased from Voigt Global Distribution Inc. (catalog # Difco-232100, Lawrence, KS), and equine chorionic gonadotropin (eCG) was purchased from the Lee BioSolutions (catalog # 493, St. Louis, Missouri). Human chorionic gonadotropin (hCG), D-(+)-raffinose pentahydrate, α-monothioglycerol (MTG), methyl-β-cyclodextrin (MBCD), reduced L-glutathione (GSH), bovine serum albumin (BSA, embryo tested), polyvinyl alcohol (PVA) and embryo tested water were purchased from Sigma-Aldrich Corp. (St. Louis, Missouri). M2 medium was purchased from LifeGlobal Group (catalog #ZFM2, http://www.ivfonline.com ), and Research Vitro Fert (RVF) medium was purchased from Cook Medical, Inc. (Bloomington, Indiana). For IVF, the RVF medium was modified with addition CaCl2 to increase the Ca2+ concentration from 2.04 mM (regular concentration) to 5.14 mM (high concentration). MBCD medium (TYH medium containing 1 mg/mL PVA and 0.75 mM MBCD) [11 (link)] was prepared in-house. RVF and MBCD media as well paraffin oil (Fisher Scientific, Pittsburgh, Pennsylvania) were pre-equilibrated in CO2 incubators overnight or at least 1 hour prior to use.
Equine chorionic gonadotropin
Manufactured by Lee Biosolutions
Equine chorionic gonadotropin (eCG) is a glycoprotein hormone produced by the endometrial cups of pregnant mares. It functions as a stimulator of ovarian follicular growth and maturation.
Automatically generated - may contain errors
Lab products found in correlation
Paraffin oil, by Thermo Fisher Scientific (2 mentions)
Methyl β cyclodextrin, by Merck Group (2 mentions)
α monothioglycerol mtg, by Merck Group (2 mentions)
L glutathione reduced, by Merck Group (2 mentions)
Polyvinyl alcohol (pva), by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Research vitro fert medium, by Cook Medical (2 mentions)
2 protocols using equine chorionic gonadotropin
1
Optimized IVF Media Protocols
2
Optimized IVF Media Protocols
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BD DifcoTM skim milk was purchased from Voigt Global Distribution Inc. (catalog # Difco-232100, Lawrence, KS), and equine chorionic gonadotropin (eCG) was purchased from the Lee BioSolutions (catalog # 493, St. Louis, Missouri). Human chorionic gonadotropin (hCG), D-(+)-raffinose pentahydrate, α-monothioglycerol (MTG), methyl-β-cyclodextrin (MBCD), reduced L-glutathione (GSH), bovine serum albumin (BSA, embryo tested), polyvinyl alcohol (PVA) and embryo tested water were purchased from Sigma-Aldrich Corp. (St. Louis, Missouri). M2 medium was purchased from LifeGlobal Group (catalog #ZFM2, http://www.ivfonline.com ), and Research Vitro Fert (RVF) medium was purchased from Cook Medical, Inc. (Bloomington, Indiana). For IVF, the RVF medium was modified with addition CaCl2 to increase the Ca2+ concentration from 2.04 mM (regular concentration) to 5.14 mM (high concentration). MBCD medium (TYH medium containing 1 mg/mL PVA and 0.75 mM MBCD) [11 (link)] was prepared in-house. RVF and MBCD media as well paraffin oil (Fisher Scientific, Pittsburgh, Pennsylvania) were pre-equilibrated in CO2 incubators overnight or at least 1 hour prior to use.
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