B6 cd45.1 mice

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B6 CD45.1 mice are a transgenic mouse strain that express the CD45.1 allele on a C57BL/6 genetic background. The CD45.1 allele is a commonly used congenic marker that allows for the tracking and identification of cells derived from these mice in various experimental and research applications.

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22 protocols using b6 cd45.1 mice

Aging and Germ-Free Murine Models

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Young C57BL/6 (B6), BALB/c, and B6.CD45.1 mice were obtained from the Jackson Laboratory. Aged (8–12 weeks) C57BL/6 (B6), BALB/c mice were obtained from the National Institute of Aging (NIA). SAP^−/−.CD45.1 mice and maintained in standard specific pathogen free (SPF) housing at the Umass Chan facility. MD4. Ig‐tg mice were obtained from Dr. Andrea Reboldi, bred with B6.CD45.1 mice and maintained in standard specific pathogen free (SPF) housing at the Umass Chan facility. BALB/c.TLR7^−/−, BALB/c TLR9^−/−, and Unc93b^−/− mice were obtained from Dr. Ann Rothstein and these strains were bred and maintained at the Umass Chan animal facility. Young and aged germ‐free C57BL/6 mice were bred and maintained in the Gnotobiotic Core at the College of Veterinary Medicine, North Carolina State University. Germ‐free mice were housed in flexible film isolators and provided with autoclaved food and water. Germ‐free status was evaluated at least once a month by culturing stool samples in aerobic and anaerobic conditions. Aged SPF and GF mice were at least 72 weeks old and young mice were at least 8 weeks old prior to use. Animal use protocols were approved by the IACUCs at Umass Chan and at North Carolina State University.

Kugler‐Umana O., Zhang W., Kuang Y., Liang J., Castonguay C.H., Tonkonogy S.L., Marshak‐Rothstein A., Devarajan P, & Swain S.L. (2022). IgD + age‐associated B cells are the progenitors of the main T‐independent B cell response to infection that generates protective Ab and can be induced by an inactivated vaccine in the aged. Aging Cell, 21(10), e13705.

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Transgenic Mouse Models for Dendritic Spine Imaging

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Thy1-YFP-H mice (stock 003782)^{57 (link)}, which express yellow fluorescent protein (YFP) in L5 pyramidal neurons, Rosa26-stop-DTR mice (stock 007900)^{58 (link)}, which express a conditional DTR-encoding allele in the ROSA locus, Rag1^−/− mice (stock 002216)^{59 (link)}, Tnf^−/− mice (stock 005540)^{60 (link)}, Il6^−/− mice (stock 002650) and B6 CD45.1 mice (stock 002014) were purchased from the Jackson Laboratory. Cx3cr1^CreER mice^{35 (link)} were obtained from the laboratory of Wen-Biao Gan (New York University). Cx3cr1^GFP mice^{34 (link)} were obtained from the laboratory of Dan Littman (New York University). To image dendritic spine plasticity in Cx3cr1^CreER/+;R26^iDTR/+ and Tnf^−/− mice, these mice were crossed to Thy1-YFP-H mice. For systemic immune challenge, mice were administered poly(I:C) (Sigma P1530; by i.p. injection) that was dissolved in DPBS. Mice were group-housed in temperature-controlled rooms on a 12-h light–dark cycle and were randomly assigned to different treatment groups. The group size was determined based on previous studies using the same methodologies^{28 (link),29 (link),30 (link),31 (link),32 (link)}. Both male and female mice were used. All mice were maintained at the NYU Skirball Institute specific-pathogen-free animal facility and handled in accordance with the institutional guidelines for animal care and use.

Garré J.M., Silva H.M., Lafaille J.J, & Yang G. (2017). CX3CR1+ monocytes modulate learning and learning-dependent dendritic spine remodeling via TNFα. Nature medicine, 23(6), 714-722.

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AhR Knockout Mice for Immunological Studies

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AhR^+/− mice lacking exon 2 on a C57BL/6 background were obtained from C. Bradfield (University of Wisconsin-Madison, Madison, WI) and also were purchased from The Jackson Laboratory (which is the provider of the same mice originally donated by C. Bradfield). C57BL/6 and B6 CD45.1 mice were obtained from The Jackson Laboratory. AhR^+/+ and AhR^−/− mice were bred from AhR^+/− mice and maintained as separate colonies. B10;B6 Rag2^−/−γ^−/− mice were obtained from Taconic. Mice of both sexes were used for experiments at age 8–12 wk. All animal procedures were performed in accordance with protocols approved by the Administrative Panel on Laboratory Animal Care at Stanford University.

Zhang L.H., Shin J.H., Haggadone M.D, & Sunwoo J.B. (2016). The aryl hydrocarbon receptor is required for the maintenance of liver-resident natural killer cells. The Journal of Experimental Medicine, 213(11), 2249-2257.

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Characterization of Arginine Metabolism Mice

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Arg1^flox mice (strain # 008817), CD4cre mice (strain # 022071), Arg2 KO mice (strain # 020286), Rag1 KO (strain # 002216) and B6 CD45.1 mice (strain # 002014) are all on the C57BL/6J background and were obtained from Jackson Laboratories.^{18 (link),73 (link)–77 (link)} The Arg1^flox mice were bred to the CD4cre mice to generate Arg1^fl/fl CD4cre⁺ mice. The C57BL/10 Rag2^−/− mice were obtained from Taconic. The appropriate littermate mice were used as WT controls (WT, Arg1^fl/fl CD4cre⁻ or WT CD4cre⁺). Both female and male mice were used to equal parts and all mice were housed at ~22 °C. All mice were maintained in BSL2 AALAC-accredited facilities at the NIH and were performed in compliance with an animal study proposal approved by the NHLBI Animal Care and Use Committee.

West E.E., Merle N.S., Kamiński M.M., Palacios G., Kumar D., Wang L., Bibby J.A., Overdahl K., Jarmusch A.K., Freeley S., Lee D.Y., Thompson J.W., Yu Z.X., Taylor N., Sitbon M., Green D.R., Bohrer A., Mayer-Barber K.D., Afzali B., Kazemian M., Scholl-Buergi S., Karall D., Huemer M, & Kemper C. (2023). Loss of CD4+ T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection. Immunity, 56(9), 2036-2053.e12.

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Treg Cell Breeding and Characterization

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Male mice of various ages were produced in our specific-pathogen-free facilities at Harvard Medical School, and were fed a chow diet (no. 5058, Lab Diet Picolab Mouse Diet 20), or HFD (60 kcal% Fat, Research Diets, D12492i). B6.CD45.1⁺ mice were purchased from the Jackson Laboratory. Foxp3-GFP mice were obtained from Dr. V. Kuchroo. vTreg53 TCR-tg mice were generated based on one of the expanded VAT-Treg clones, as previously described (Li et al., 2018 (link)), and were crossed with Foxp3-GFP^KI/KI females. Tg⁺ Foxp3-GFP^KI/y male progeny were used for experiments. In all cases, the Tcra plus Tcrb transgene was maintained in the heterozygous state. All experiments were performed using littermate controls and following animal protocols approved by the HMS Institutional Animal Use and Care Committee (protocol IS00001257).

Fernandes R.A., Li C., Wang G., Yang X., Savvides C.S., Glassman C.R., Dong S., Luxenberg E., Sibener L.V., Birnbaum M.E., Benoist C., Mathis D, & Garcia K.C. (2020). Discovery of surrogate agonists for visceral fat Treg cells that modulate metabolic indices in vivo. eLife, 9, e58463.

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Melanoma Tumor Induction in B6 Mice

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C57Bl/6 or B6 CD45.1 mice were purchased at 6 weeks of age from the Jackson Laboratory (Bar Harbor, ME, USA). All protocols were approved by the Institutional Animal Care and Use Committee (IACUC). For tumor-bearing cohorts, 0.5 × 10⁶ melanoma cells were intradermally implanted into the left dorsal skin of 6- to 8-week-old mice. Tumor dimensions were measured with calipers in three dimensions and reported as an ellipsoidal volume.

O’Melia M.J., Rohner N.A., Manspeaker M.P., Francis D.M., Kissick H.T, & Thomas S.N. (2020). Quality of CD8+ T cell immunity evoked in lymph nodes is compartmentalized by route of antigen transport and functional in tumor context. Science Advances, 6(50), eabd7134.

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Characterizing TCRα Repertoire in B6 Mice

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Wild-type (WT) C57BL/6 (B6) mice were purchased from Charles River Laboratories; B6.CD45.1 mice were from The Jackson Laboratory; the new Traj18^−/− mice expressing undisturbed TCRα chain repertoire, except for Jα18, on B6 background were described (20 (link)). Mice were maintained in the animal facility of RIKEN IMS under specific pathogen-free conditions and were used at 8–10 weeks of age. All animal experiments were approved by RIKEN Animal Care and Use Committee.

Dashtsoodol N., Shigeura T., Tashiro T., Aihara M., Chikanishi T., Okada H., Hanada K., Sano H., Kurogi A, & Taniguchi M. (2017). Natural Killer T Cell-Targeted Immunotherapy Mediating Long-term Memory Responses and Strong Antitumor Activity. Frontiers in Immunology, 8, 1206.

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Transgenic Mouse Models for Dendritic Spine Imaging

Cited 3 times

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Garré J.M., Silva H.M., Lafaille J.J, & Yang G. (2017). CX3CR1+ monocytes modulate learning and learning-dependent dendritic spine remodeling via TNFα. Nature medicine, 23(6), 714-722.

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Generating Bone Marrow Chimeric Mice

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C57BL/6J (B6, CD45.2) and congenic B6 CD45.1⁺ mice were from the Jackson Laboratory, and these strains were intercrossed to generate B6 CD45.1/2 F1 mice. Gpr18^−/− mice were generated as described (14 (link)) To generate bone marrow (BM) chimeras, CD45.1⁺ B6 mice were irradiated by exposure to 1,100 rad of γ-irradiation in two doses 5 h apart and i.v. injected with at least 2 × 10⁶ total BM cells from each genotype of mice as indicated and analyzed after 2–3 months. All chimeras appeared healthy at the time of analysis. For LCMV infection, mice were infected with 2 × 10⁵ plaque-forming units of LCMV Armstrong administered i.v. Animals were housed in a specific pathogen-free environment in the Laboratory Animal Research Center at the UCSF, and all experiments conformed to ethical principles and guidelines approved by the UCSF Institutional Animal Care and Use Committee.

Sumida H, & Cyster J.G. (2018). G-Protein Coupled Receptor 18 Contributes to Establishment of the CD8 Effector T Cell Compartment. Frontiers in Immunology, 9, 660.

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Adoptive T-cell Transfer in Irradiated Mice

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B6.CD45.1⁺ mice were obtained from The Jackson Laboratory and subjected to a 500rad irradiation. Daxx^+/+ and Daxx^-/- donor T cells (CD45.2⁺) were isolated from the spleen and lymph nodes of indicated mice. T cells were resuspended in PBS and adoptively transferred into irradiated CD45.1⁺ recipient mice (3 x 10⁶ cells/mouse) by retroorbital injection. Seven days later, spleen and lymph nodes of recipient mice were made into single cell suspensions, stained with anti-CD45.1⁺ and CD45.2⁺ (eBioscience) and anti-Thy1.2 (BD Biosciences) antibodies, and analyzed by flow cytometry on an LSR II.

Li J., Qian L., Dowling J.P., Curcione C., Kurup D, & Zhang J. (2017). Daxx plays a novel role in T cell survival but is dispensable in Fas-induced apoptosis. PLoS ONE, 12(3), e0174011.

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