F240fc a

Manufactured by Thorlabs

Sourced in Japan

The F240FC-A is a Ø1/2" (Ø12.7 mm) Achromatic Doublet Lens with a Focal Length of 40.0 mm and AR Coating. It is a type of optical lens used to focus or collimate light.

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Lab products found in correlation

Md498, by Thorlabs (3 mentions) Mf525 39, by Thorlabs (3 mentions) Zirconia sleeve, by Doric (1 mentions) Ni usb 6259, by National Instruments (1 mentions) Mf469 35, by Thorlabs (1 mentions) Model sr830, by Stanford Research Systems (1 mentions) R3896, by Hamamatsu Photonics (1 mentions) C7319, by Hamamatsu Photonics (1 mentions) Fp400urt, by Thorlabs (1 mentions) T495lpxr, by Chroma Technology (1 mentions) Ni usb 6341, by National Instruments (1 mentions)

4 protocols using f240fc a

Fiber Photometry Setup for GCaMP6f Imaging

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Fiber photometry data were collected and analyzed using a custom-made photometry setup and Matlab-based software. We used a 470-nm LED source (M470F3, Thorlabs) coupled to an optic fiber (M75L01) and collimation lens (F240FC-A) for GCaMP6f excitation. The 470-nm excitation light was delivered to the cannula implanted on the head of the animal using a second collimation lens (F240FC-A) coupled to a 400 µm, high NA, low autofluorescence optic fiber (FP400URT, custom made, Thorlabs). The emission light was collected using the same optic fiber and directed to a Newport 2151 photoreceiver using a focusing lens (ACL2541U-A, Thorlabs). Excitation (ET470/24 M) and emission (ET525/50) filters, and a dichroic mirror (T495LPXR) were purchased from Chroma Technology. The 470-nm excitation light was amplitude-modulated at a frequency of 211 Hz, with a max power of 40 µW, using an LED driver (LEDD1B) controlled through a National Instrument DAQ (NI USB-6341). The modulated data acquired from the photoreceiver were decoded as in Lerner et al., 2015 (link) using a custom Matlab function (available at https://github.com/QuentinNeuro/Bpod-FunctionQC, copy archived at swh:1:rev:1ea70f47f0bd5fbf5441abe0dac3dc70ed3c9a8b; Chevy, 2021 ; Ibrahim et al., 2013 (link)).

Szadai Z., Pi H.J., Chevy Q., Ócsai K., Albeanu D.F., Chiovini B., Szalay G., Katona G., Kepecs A, & Rózsa B. (2022). Cortex-wide response mode of VIP-expressing inhibitory neurons by reward and punishment. eLife, 11, e78815.

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Fiber Photometry Recordings for GCaMP6f Fluorescence

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Fiber photometry recordings were performed similarly to those in ref. 25 (link). Briefly, we sinusoidally modulated blue light from a 470-nm excitation LED (M470F3, Thorlabs, NJ, USA) at 211 Hz, using a custom Matlab program (MathWorks, Natick, MA, USA) and a multifunction data acquisition device (NI USB-6259, National Instruments, Austin, TX, USA). The blue light was passed through a GFP excitation filter (MF469-35, Thorlabs), reflected off a dichroic mirror (MD498, Thorlabs), and coupled using a fiber collimation package (F240FC-A, Thorlabs) into a low-fluorescence patch cord (400 μm, 0.48 NA; Doric Lenses) connected to the implanted optic fiber (400 μm, 0.48 NA; Doric Lenses) by a zirconia sleeve (Doric Lenses). GCaMP6f fluorescence was collected through the excitation patch cord, passed through a GFP emission filter (MF525-39, Thorlabs), and focused onto a photodetector (Model 2151, Newport, Irvine, CA, USA) using a lens (LA1540-A, Thorlabs). The signal was sent to a lock-in amplifier (30-ms time constant, Model SR830, Stanford Research Systems, Sunnyvale, CA, USA) that was synchronized to 211 Hz. Signals from the amplifiers were collected at 1 KHz using a custom Matlab program and a multifunction data acquisition device (National Instruments).

Eban-Rothschild A., Rothschild G., Giardino W.J., Jones J.R, & de Lecea L. (2016). VTA dopaminergic neurons regulate ethologically relevant sleep–wake behaviors. Nature neuroscience, 19(10), 1356-1366.

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Fiber Photometry for Real-Time GCaMP6 Monitoring

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Following AAV-EF1α-DIO-GCaMP6f virus injection, an optical fiber (125 µm O.D., 0.37 numerical aperture (NA); Newdoon, Shanghai) was placed in a ceramic ferrule and inserted towards the NAc. Fiber photometry^{53 (link)} uses the same fiber to both excite and record from GCaMP in real time. To record fluorescence signals, laser beam was passed through a 488 nm laser (OBIS 488LS; Coherent), reflected off a dichroic mirror (MD498; Thorlabs), focused by objective lens (Olympus), and coupled through a fiber collimation package (F240FC-A, Thorlabs) into a patch cable connected to the ferrule of the upright optic fiber implanted in the mouse via a ceramic sleeve (125 µm O.D.; Newdoon, Shanghai). GCaMP6 fluorescence was bandpass filtered (MF525–39, Thorlabs) and collected by a photomultiplier tube (R3896, Hamamatsu). An amplifier (C7319, Hamamatsu) was used to convert the photomultiplier tube current output to voltage signals, which was further filtered through a low-pass filter (40 Hz cut-off; Brownlee 440). The photometry voltage traces were downsampled using interpolation to match the EEG/EMG sampling rate of 512 Hz by using a Power1401 digitizer and Spike2 software (CED, Cambridge, UK). Analysis of the resulting signal was performed with custom written MATLAB software.

Luo Y.J., Li Y.D., Wang L., Yang S.R., Yuan X.S., Wang J., Cherasse Y., Lazarus M., Chen J.F., Qu W.M, & Huang Z.L. (2018). Nucleus accumbens controls wakefulness by a subpopulation of neurons expressing dopamine D1 receptors. Nature Communications, 9, 1576.

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Fiber Photometry for Neural Activity

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After the virus injections, an optical fiber was positioned in a ceramic ferrule (230 μm in diameter, Xi 'an Bogao Optoelectronic Technology Co., LTD) and inserted into the regions of M1 and 5Cb for fiber photometry. The signals were collected by a laser beam through a 488 nm simulator (OBIS 488LS; Coherent), reflected off a dichroic mirror (MD498; Thorlabs), focused by an objective lens (Olympus, Japan), and coupled through a fiber collimation package (F240FC-A, Thorlabs) into a patch cable. The cable was connected to fibers chronically implanted in the mouse. Fluorescent signals were bandpass filtered (MF525-39, Thorlabs) and collected by a photomultiplier tube (CMOS, IDS imaging).
As described in previous studies 25 (link), 26 , the photometry data were imported to MATLAB R2020b MAT files for further analysis. The data was smoothed with a moving average filter and segmented based on behavioral events. The values of fluorescence change (ΔF/F) were calculated by (F-F₀)/F₀, where F₀ is the baseline fluorescence signal averaged over a 1.5 s control time window. ΔF/F values were illustrated by spectrogram or average plots with a shaded area indicating SEM.

Zhu J., Chen C., Liu X., He M., Fang Y., Wang L., Jia J., Guo J., Zhao Z., Gao C., He J., Xu C., Xu F., Ma D., Wang J, & Zhang Z. (2024). Cerebellar Purkinje cell firing promotes conscious recovery from anesthesia state through coordinating neuronal communications with motor cortex. Theranostics, 14(2), 480-495.

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