Flag and α tubulin

Manufactured by Merck Group

Flag and α-tubulin are lab equipment used for various research applications. Flag is a molecular tag used to identify and purify specific proteins, while α-tubulin is a structural protein found in the cytoskeleton of eukaryotic cells. Both are commonly used in cell biology and biochemistry experiments, but a detailed description of their core functions without extrapolation is not available.

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Lab products found in correlation

C myc, by Cell Signaling Technology (2 mentions) Glial fibrillary acidic protein (gfap), by BioLegend (2 mentions) Ubiquitin, by BioLegend (1 mentions) Usp13, by Abcam (1 mentions) Cd133, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

α-tubulin (1242 citations) α-FLAG (120 citations) TUBULIN and FLAG (4 citations) Anti-α-tubulin and anti-FLAG M2 antibodies (2 citations)

2 protocols using flag and α tubulin

Protein Expression and Immunofluorescence Staining Protocol

Cited 3 times

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IB analysis of protein expression and immunofluorescence staining on cells or tissue sections were performed as previously described (Bao et al., 2006a (link); Guryanova et al., 2011 (link); Cheng et al., 2013 (link); Zhou et al., 2015 (link)). Specific antibodies against USP13 (Abcam), c-Myc (Cell Signaling Technology or Santa Cruz Biotechnology, Inc.), SOX2 and OLIG2 (EMD Millipore or Santa Cruz Biotechnology, Inc.), Flag and α-tubulin (Sigma-Aldrich), GFAP (BioLegend or BD), MAP2 (Covance), TUJ1 (Covance), CD31 (Dako), FBXL14 (Santa Cruz Biotechnology, Inc.), CD133 (Miltenyi Biotec), ubiquitin (BioLegend), hemagglutinin (HA; Santa Cruz Biotechnology, Inc.), and Ki-67 (Abcam) were used for IB analysis or immunofluorescent staining. IHC staining on tumor and normal tissue sections was performed with an avidin–biotin complex kit and a 3,3′-diaminobenzine detection kit (Vector Laboratories) as previously described (Bao et al., 2006b (link); Guryanova et al., 2011 (link); Zhou et al., 2015 (link)).

Fang X., Zhou W., Wu Q., Huang Z., Shi Y., Yang K., Chen C., Xie Q., Mack S.C., Wang X., Carcaboso A.M., Sloan A.E., Ouyang G., McLendon R.E., Bian X.W., Rich J.N, & Bao S. (2017). Deubiquitinase USP13 maintains glioblastoma stem cells by antagonizing FBXL14-mediated Myc ubiquitination. The Journal of Experimental Medicine, 214(1), 245-267.

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Protein Expression and Immunostaining Protocol

Cited 2 times

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IB analysis of protein expression and IF staining of cells or tissue sections were performed as described [8 (link), 20 (link), 43 (link)]. Specific antibodies against ZFX (Cell Signaling or Sigma-Aldrich), c-Myc (Cell Signaling or Santa Cruz), SOX2 and OLIG2 (Millipore or Santa cruse), Flag and α-tubulin (Sigma-Aldrich), GFAP (Biolegend or BD Bioscience), MAP2 (Covance), TUJ1 (Covance), CD31 (Dako), Tbx3 (Abcam) and Tcl1 (Norvus) were used for IB analysis or IF staining. IHC staining on tumor and normal tissue sections was performed with an ABC kit using DAB (3,3’-Diaminobenzine) detection (Vector Lab) as previously described [15 (link), 43 (link)]. A specific antibody against ZFX (Sigma-Aldrich) was used for IHC on paraffin embedded tumor and normal brain tissue sections including tissue microarrays (US Biomax).

Fang X., Huang Z., Zhou W., Wu Q., Sloan A.E., Ouyang G., Mclendon R.E., Yu J.S., Rich J.N, & Bao S. (2014). The Zinc Finger Transcription Factor ZFX is Required for Maintaining the Tumorigenic Potential of Glioblastoma Stem Cells. Stem cells (Dayton, Ohio), 32(8), 2033-2047.

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