For bud samples, 250 μL pyridine (Sigma-Aldrich, St. Louis, MO) and 250 μL STOX solution (For 750 μg/vial internal standard: 100 mL pyridine, 2.5 g hydroxylamine hydrochloride, and 0.6 g phenyl-β-D-glucopyranoside; For 100 μg/vial internal standard: 100 mL pyridine, 2.5 g hydroxylamine hydrochloride, and 80 mg phenyl-β-D-glucopyranoside) were added to each dried glass vial. The vials were shaken for 10 sec and placed on the Reacti-Therm Heating Modules at 70°C for 40 min. Vials were then removed from the heating block and cooled under room temperature. Four hundred μL hexamethyldisilazane (HMDS, Sigma-Aldrich, St. Louis, MO) and 40 μL trifluoroacetic acid (TFA, Sigma-Aldrich, St. Louis, MO) were added to each glass vial and the vials were shaken for 10 sec. Finally, the vials were placed at 4°C refrigerator overnight for precipitation. The supernatants were transferred to 1.5 mL vials and ready for sugar analysis the next day. Leaf samples were derivatized following the same procedure except the volumes for pyridine, STOX solution, HMDS, and TFA were 125, 125, 200, and 20 μL respectively.
Hydroxylamine hydrochloride
Manufactured by Merck Group
Sourced in United States, Germany, India, China, United Kingdom, Italy
Hydroxylamine hydrochloride is a chemical compound commonly used in various laboratory applications. It is a white crystalline solid that is soluble in water and other polar solvents. The primary function of hydroxylamine hydrochloride is to serve as a reducing agent and a precursor in organic synthesis reactions.
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Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (30 mentions)
Hydrochloric acid (hcl), by Merck Group (23 mentions)
Silver nitrate, by Merck Group (18 mentions)
Sodium borohydride, by Merck Group (16 mentions)
Ethanol, by Merck Group (13 mentions)
Methanol, by Merck Group (12 mentions)
Pyridine, by Merck Group (12 mentions)
Sodium chloride (nacl), by Merck Group (11 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (10 mentions)
Bovine serum albumin (bsa), by Merck Group (10 mentions)
Sodium acetate, by Merck Group (9 mentions)
Tetraethyl orthosilicate, by Merck Group (8 mentions)
Quercetin, by Merck Group (7 mentions)
Gold 3 chloride trihydrate, by Merck Group (7 mentions)
Acetic acid, by Merck Group (7 mentions)
Trichloroacetic acid, by Merck Group (7 mentions)
Sodium carbonate, by Merck Group (7 mentions)
Sodium citrate, by Merck Group (6 mentions)
Nitric acid, by Merck Group (6 mentions)
Triton x 100, by Merck Group (6 mentions)
176 protocols using hydroxylamine hydrochloride
1
Derivatization of Plant Bud and Leaf Samples
2
Arsenic Adsorption by PS and LDPE Resins
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PS and LDPE resin pellets (3 mm in particle size) purchased from Sigma-Aldrich Australia were used as the adsorbents. Multi-element standard solution 4 for ICP (40 mg/L of As) was supplied by Sigma-Aldrich Pty Ltd., Australia. The solution was diluted by deionized water with a resistivity of 18 MΩ. Analytical grade sodium arsenate dibasic heptahydrate (Na2HAsO4⋅7H20) and sodium arsenite (NaAsO2), purchased from Sigma-Aldrich Pty Ltd, were dissolved with deionized water to obtain As(III) and As(V) stock solutions (100 mg/L). The phosphoric acid (H3PO4, 85% w/w) and hydroxylamine hydrochloride (NH2OH⋅HCl, 99% purity) were also obtained from Sigma-Aldrich Pty Ltd. Then, solutions of 1.0 M phosphoric acid and 0.2 M hydroxylamine hydrochloride were prepared by diluting their original standard solution with deionized water. All plasticware and glassware were soaked in 2% (v/v) HNO3, followed by repeated rinsing with deionized water, and then dried before use.
3
Multifunctional Nanoparticle Synthesis
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FeCl2.4H2O and FeCl3.6H2O were purchased from Merck, Germany. HAuCl4.3H2O, tetramethylammonium hydroxide (TMAOH), sulfadiazine, PAA (Mw=1800 g mol-1, R & D application), (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), polyethylenimine (PEI), Iodine, and potassium iodide were received from Sigma, USA. 4-Dimethylaminopyridine (DMAP), sodium borohydride, epichlorohydrin, sodium citrate, cystamine dihydrochloride, hydroxylamine hydrochloride, dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) were obtained from Merck, Germany. MCF-7 and L-929 cell lines were purchased from Pasteur Institute of Iran, Tehran, Iran.
4
Synthesis of Choline-Decorated Polymeric Nanoparticles
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Tetrachloroauric acid (HAuCl4*3H2O) ≥ 99.9%, molar weight: 393.83 g/mol; βCD (C42H70O35) ≥ 98%, 1134.98 g/mol; (±) epichlorohydrin (EP, C3H5ClO) ≥ 99%, 92.52 g/mol; choline chloride (CC, C5H14ONCl) ≥ 98%, 139.62 g/mol; PhEA (1-phenylethan-2-amine, C8H11N) ≥ 99%, 121.18 g/mol, δ: 0.962 g/mL; PIP ((2E,4E)-5-(2H-1,3-benzodioxol-5-yl)-1-(piperidin-1-yl)penta-2,4-dien-1-one, C17H19NO3) ≥ 97%, 285.3 g/mol; and sodium hydroxide (NaOH) ≥ 97%, 40.00 g/mol were provided by Sigma Aldrich (Saint Louis, MO, USA). Hydroxylamine hydrochloride (NH2OH*HCl) ≥ 98%, 69.49 g/mol; hydrochloric acid (HCl) for analysis, 36.46 g/mol; and water (nanopure) were provided by Merck (Darmstadt, Germany).
5
Comprehensive Antioxidant Characterization Protocol
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All the chemicals, including Folin-Ciocalteu’s phenol reagent, polyvinylpyrrolidone (PVP), sodium carbonate, tannic acid (98% purity), aluminum chloride hexahydrate (AlCl3·6H2O; Ph Eur purity), quercetin (98% purity), 1,1-diphenyl-2-picrylhydrazyl radical (DPPH; 95% purity), 2,2-azobis (2-methylpropionamidine) dihydrochloride (AAPH; 97% purity), 2,2-azino-bis (3-thylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS; 98% purity), trolox (97% purity), ferrozine (97% purity), iron (II) sulfate heptahydrate (FeSO4·7H2O; 99% purity), iron (III) chloride (FeCl3·6H2O; 97% purity), hydroxylamine hydrochloride (98% purity), rutin (99% purity), bovine serum albumin, glucose, fructose, sodium azide, iron (II) chloride (FeCl2·4H2O; 99% purity), tert-butyl hydroperoxide (tBOOH; 80% purity), and methanol were purchased from Merck (Darmstadt, Germany). RPMI 1640 medium and fetal bovine serum were provided by Aurogene (Rome, Italy).
6
Synthesis and Characterization of Organic Compounds
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Dimethyl terephthalate (qNMR), furfuryl alcohol (96%w), maleic anhydride (99%w), hydroxylamine hydrochloride (99%w), triethanolamine (99%w), hydrochloric acid (37%w) and DMSO (98%) were purchased from Merck. VWR provided the ethanol (96%v). The d6-DMSO was purchased from Eurisotop. The water used in the synthesis and characterization was distilled using a SI Analytics distillation unit.
7
Synthesis of Metal Nanoparticles
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Gold(III)chloride trihydrate, trisodium citrate dihydrate, hydroxylamine hydrochloride, glyphosate (N-(phosphonomethyl)glycine) powder, sodium nitrate, and cysteamine hydrochloride (≥98%) were provided by Merck. Silver nitrate was provided by Kemika (Zagreb, Croatia). All chemicals were of analytical grade and were used without further processing. High purity water with a resistivity of 18 MΩ cm−1 was used for all experiments.
8
Cytoskeleton Disruption Assay with Small Molecules
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Paclitaxel, vinblastine sulphate, podophyllotoxin, colchicine, 5,5′‐dithiobis‐2‐nitrobenzoic acid (DTNB), sulforhodamine B (SRB), Hoechst 33342, guanosine 5′‐triphosphate (GTP), propidium iodide, EGTA, MgCl2, piperazine‐N,N′‐bis (2‐ethanesulphonic acid) (PIPES), mouse monoclonal anti‐α‐tubulin IgG and FITC‐conjugated anti‐mouse IgG, fluorescein isothiocyanate isomer (FITC) and dimethylformamide were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Foetal bovine serum (FBS) and Alexa Fluor 568‐conjugated anti‐mouse IgG were purchased from Invitrogen (Thermo Scientific, Massachusetts, USA). Acridine orange (AO), hydroxysuccinimide, minimal essential medium (MEM), cell culture tested antibiotic solution, and phosphate‐buffered saline (PBS) were purchased from HiMedia (Mumbai, India). Dichloromethane, n‐hexane, hydroxylamine hydrochloride and triethylamine were purchased from Merck, India. 1‐Ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride was purchased from SRL chemicals. All other reagents used in the study were of analytical grade.
9
Cellulose Microfibrils from Cotton Flower
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Cotton flower (obtained from Yazd, Iran) was chopped and treated with water, ethanol, and 18% NaOH to give cellulose microfibrils (MC). H2SO4, monosodium phosphate, disodium phosphate, Tris–acetate buffer, acetic acid, sodium chlorite (NaClO2), sodium periodate (NaIO4), hydrazine hydrate, hydroxylamine hydrochloride, urea, aldehydes, and ethyl acetoacetate were supplied by Merck (Germany). All materials were reagent grade and used without further purification, except the urease with absolute activity 50 U mg−1 which was extracted from soya beans and purified according to previously reported procedures.13,46 (link)
10
Mercury Analysis Protocol for Environmental Samples
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Mercury standards of 1000 mg L -1 from High Purity Standards (USA) were used to prepare the calibration standards, spike standard and independent standard.
Ferrous sulfate (FeSO4•7H2O) from AJAX Chemicals (Australia) was used to prepare the Fe solution. Sodium borohydride, sodium hydroxide, ultrapure nitric acid and hydrochloric acid were all from Merck (Australia). Sodium bromate and sodium bromide were from Merck (Australia) and were baked in a furnace at 200°C overnight before preparation of BrCl. Hydroxylamine hydrochloride was also from Merck (Australia). The laboratory deionized (DI) water used for standard and reagent preparation was generated from a Millipore water system.
The digestion reagent was prepared using the ratio of 10.8 g of potassium bromide (KBr) and 15.2 g of potassium bromate (KBrO3) per 1000 mL of concentrated hydrochloric acid. The Hydroxylamine hydrochloride solution was prepared by dissolving 15 g of Hydroxylamine hydrochloride (NH2OH• HCl) in approximately 25 mL of DI water and made-up to a final volume of 50 mL.
The reductant solution is 0.4% w/v NaBH4 and 0.05% w/v NaOH. The carrier solution is 3% v/v HCl in DI water. The mercury calibration standard and spike standard were prepared in 1% v/v HNO3 DI water.
Ferrous sulfate (FeSO4•7H2O) from AJAX Chemicals (Australia) was used to prepare the Fe solution. Sodium borohydride, sodium hydroxide, ultrapure nitric acid and hydrochloric acid were all from Merck (Australia). Sodium bromate and sodium bromide were from Merck (Australia) and were baked in a furnace at 200°C overnight before preparation of BrCl. Hydroxylamine hydrochloride was also from Merck (Australia). The laboratory deionized (DI) water used for standard and reagent preparation was generated from a Millipore water system.
The digestion reagent was prepared using the ratio of 10.8 g of potassium bromide (KBr) and 15.2 g of potassium bromate (KBrO3) per 1000 mL of concentrated hydrochloric acid. The Hydroxylamine hydrochloride solution was prepared by dissolving 15 g of Hydroxylamine hydrochloride (NH2OH• HCl) in approximately 25 mL of DI water and made-up to a final volume of 50 mL.
The reductant solution is 0.4% w/v NaBH4 and 0.05% w/v NaOH. The carrier solution is 3% v/v HCl in DI water. The mercury calibration standard and spike standard were prepared in 1% v/v HNO3 DI water.
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