HFD (TP23400, 60% high-fat model) was ordered from Nantong Trophy Feed (Nantong, Jiangsu, China). Sophoridine (HPLC, ≥98.6%, CAS: 6882-68-4) was purchased from Chengdu Durst (Chengdu, China). Antibodies against peroxisome proliferator-activated receptor gamma (PPARγ, ab209350) and CCAAT enhancer binding protein alpha (C/EBPα, ab32358) were purchased from Abcam. Antibodies against adipose triglyceride lipase (ATGL, 2138) and glucose transporter 4 (GLUT4, 2213) were purchased from Cell Signaling (Danvers, MA, USA). Antibodies against fatty acid binding protein 4 (FABP4, sc-271529), cyclinB (sc-166152), P21 (sc-6246), cyclin-dependent kinase 4 (CDK4, sc-23896), cAMP-response element binding protein (sc-377154), and β-actin (sc-47778) were obtained from Santa Cruz (Dallas, TX, USA). Antibodies against CCAAT enhancer binding protein beta (CEBPβ, CY5065), cyclinD1 (CY5404), cyclinE2 (CY5821), c-Src (CY5459), vascular endothelial growth factor (VEGF, CY10042), vascular endothelial growth factor receptor 2 (VEGFR2, CY5385), phosphoinositide-3 kinase P85 alpha (P85α-PI3K, CY5355), AKT1/2/3 (CY5561), ERK1/2 (CY5487), P-P85α-PI3K (CY6427), P-AKT1/2 (Tyr315/316/312, AY0423), and P-ERK1/2 (Tyr185/Y187, CY5044) were purchased from Abways (Shanghai, China).
Sc 377154
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The sc-377154 is a laboratory equipment product from Santa Cruz Biotechnology. It is designed to perform a specific core function within the laboratory setting. A detailed and unbiased description of the product's features and capabilities is not available at this time.
Automatically generated - may contain errors
2 protocols using sc 377154
1
High-Fat Diet and Adipogenesis Regulation
2
Protein Analysis for Neurobiology Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Author manuscript published in final edited form as: Neurobiology of Learning and Memory. 2018; 151:35-42. doi: 10.1016 /j.nlm.2018.03.023 Protein extraction and western blot analysis were performed as described elsewhere (Serrano et al., 2012; Vida et al., 2013) . The primary antibodies used were rabbit anti-BDNF (diluted 1:200, SAB2108004, Sigma-Aldrich), mouse anti-CREB-1 (1:1000, sc-377154, Santa Cruz Biotechnology), goat anti-pCREB1 (1:100, Ser133; sc-7978, Santa Cruz Biotechnology), rabbit anti-IGF-II (1:250, sc-5622, Santa Cruz Biotechnology) and mouse anti-vinculin (1:100, sc-25336, Santa Cruz Biotechnology) as a housekeeping protein. The images were analyzed by densitometry using the ImageJ software.
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