Pro mouse cytokine 23 plex kit

Whole blood was collected from B6 mice at days 4, 8,12, and 32 of infection and compared with mock controls. Serum was isolated and inactivated, as described in our previous study [23 (link), 24 (link)]. The Pro Mouse Cytokine 23-Plex Kit (Bio-Rad) was used to measure cytokine and chemokine levels. This kit tested for IL-1α, IL-β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12(p40), IL-12(p70), IL-13, IL-17, Eotaxin, G-CSF, GM-CSF, IFN-γ, KC, MCP-1, MIP-1α, MIP-1β, RANTES, and TNFα. The Bio-Rad Bio-Plex Plate Washer and Bio-Plex 200 machines were used for sample processing and analysis. All processes were completed following the manufacturer’s instructions.

Whole blood was collected from CD-1 and B6 mice at days 2, 4, 6, 8, 10, and 12 of infection and compared with mock controls. Serum was isolated and inactivated, as described in our previous study [46 (link)]. The Pro Mouse Cytokine 23-Plex Kit (Bio-Rad) was used to measure cytokine and chemokine levels. The Bio-Rad Bio-Plex Plate Washer and Bio-Plex 200 machines were used for sample processing and analysis. All processes were completed following the manufacturer’s instructions.