C2C12 cells were preserved by our laboratory. Dried grubs were obtained from Tongrentang Chinese Medicine (China). Papain, low melt agarose (A8350), normal melting point agarose (A8201), goat serum (SL038), DAPI (C0065), RIPA buffer (R0010) was purchased from Solarbio (Beijing, China). Dulbecco’s modified Eagle’s media (DMEM) (Sigma–Aldrich, D0822), Tertiary butylhydroquinone (TBHQ) (Sigma–Aldrich, 112941), MTT was purchased from Sigma–Aldrich (M2003, St. Louis, USA). Fetal bovine serum (FBS) was purchased from Gibco Life Technologies (Australia). TRIzol reagent was obtained from Invitrogen (15596018, Shanghai, China). Antibodies against NRF2 (EP1808Y), HO-1 (EP1391Y) and β-actin (ab8227) were purchased from Abcam (Cambridge, UK). Lamin B antibody was obtained from Boster (PB9611, Wuhan, China). SOD (A001-1-2), CAT (A007-1-1), GSH-Px (A005-1-2) and Malondialdehyde (MDA) (A003-1-2) kits were purchased from Nanjing Jiancheng Biological Product (Nanjing, China).
Tertiary butylhydroquinone tbhq
Manufactured by Merck Group
Sourced in United States
Tertiary butylhydroquinone (TBHQ) is a synthetic organic compound used as a food preservative and antioxidant. It is a white crystalline solid that is sparingly soluble in water and soluble in organic solvents. TBHQ functions as an antioxidant by inhibiting the oxidation of fats and oils, thereby extending the shelf-life of food products.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s media dmem, by Merck Group (1 mentions)
Ep1808y, by Abcam (1 mentions)
Lamin b antibody, by Boster Bio (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Ab8227, by Abcam (1 mentions)
Gsh px a005 1 2, by Nanjing Jiancheng (1 mentions)
Malondialdehyde mda a003 1 2, by Nanjing Jiancheng (1 mentions)
Ferrous 2 chloride, by Merck Group (1 mentions)
Ferric 3 chloride, by Merck Group (1 mentions)
Thiobarbituric acid tba, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (1 mentions)
Mw 36000 50000, by MP Biomedicals (1 mentions)
3 protocols using tertiary butylhydroquinone tbhq
1
Antioxidant Assays for C2C12 Cells
2
Antioxidant Properties of Edible Insects
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The edible insects were sourced from different provinces of South Africa: Mashonzha (G. belina) and Madzhulu (M. subhylanus) were sourced in the Vhembe district, Limpopo, and the black soldier fly larvae (H. illucens) was sourced from Cape Town, Western Cape, South Africa. The chemical reagents, 2,2 diphenyl-1-picrylhydrazyl (DPPH), 2,2′ azobis (2-methyl, 2,2-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), Ferric (III) chloride, ethylenediaminetetraacetic acid (EDTA), tertiary butyl hydroquinone (TBHQ), ferrous (II) chloride and thiobarbituric acid (TBA) were obtained from Merk (Sigma-Aldrich, Kempton Park, South Africa). All the chemicals used in this study were of analytical grade, and chemical reagents were prepared according to standard analytical procedures. Prepared reagents were stored under conditions that prevented deterioration or contamination. The water used in the study was ultrapure water purified with a Milli-Q water purification system (Millipore, Microsep, Bellville, South Africa). The ethics committee of the faculty of applied sciences gave its approval to the study (215062965/05/2021).
3
Acute Colitis Induction and Analysis
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Twelve-week-old mice were provided 3% DSS (MW 36000-50000) (MP Biomedicals, Santa Ana, CA, United States) in their daily drinking water for 4 or 6 d and were fed a normal diet during the induction of acute colitis. The disease activity index (DAI) was measured by examining weight loss, stool consistency and blood in the stool during the experiments according to previous methods[20 (link)] (Table 1 ). After DSS was induced, the mice were anesthetized by isoflurane inhalation, and blood was harvested for subsequent analysis. The organs were removed, and the colon length was measured. Colon tissue sections (0.5 mm) were stained with hematoxylin-eosin, and histopathological examinations were performed by microscopy. Colonic mucosal tissues were scraped for subsequent experiments. For the colitis model treated with tertiary butylhydroquinone (t-BHQ) (Sigma-Aldrich, St Louis, MO, United States), KO mice in the t-BHQ group were intraperitoneally injected with 50 mg/kg/d t-BHQ for 6 d and subsequently sacrificed. The experiments were performed as described above.
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