Accuri c6 flow cytometer and software

Manufactured by BD

Sourced in Japan

The Accuri C6 flow cytometer is an analytical instrument designed for the detection and analysis of cells and particles in a liquid sample. It uses lasers to illuminate the sample and detectors to measure the resulting light signals, providing information about the size, granularity, and fluorescence properties of the analyzed objects. The Accuri C6 is accompanied by software that facilitates the collection, visualization, and analysis of the acquired data.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd69 pe, by BD (1 mentions) Anti cd4 pe, by BD (1 mentions) Anti cd8 apc, by BD (1 mentions) Easysep magnet, by STEMCELL (1 mentions) Lymphoprep, by STEMCELL (1 mentions) Easysep human cd8 positive selection kit, by STEMCELL (1 mentions) Rat igg2b, by BioLegend (1 mentions) Mouse igg1, by BioLegend (1 mentions) Mouse igg2a, by BioLegend (1 mentions) Rat igg2a, by BioLegend (1 mentions) Armenian hamster igg, by BioLegend (1 mentions) Cx3cr1 sa011f11, by BioLegend (1 mentions) Edta treated vacuum tubes, by BD (1 mentions) Facsaria 2 cytometer, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Ghost dye red 780, by Cytek Biosciences (1 mentions) Mitoscreen jc 1, by BD (1 mentions) Cflow plus software, by BD (1 mentions)

Close spelling variants of this product

BD Accuri C6 (4516 citations) BD Accuri C6 flow cytometer (3652 citations) BD Accuri C6 cytometer (449 citations) BD Accuri flow cytometer (299 citations) BD Accuri (258 citations) C6 flow cytometer (218 citations) BD Accuri cytometer (54 citations) Accuri C6 flow (15 citations) BD Accuri software (14 citations) C6 Accuri software (9 citations)

6 protocols using accuri c6 flow cytometer and software

CD8+ T Cell Depletion and Antigen Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) were isolated from up to 300 ml whole blood donations using Lymphoprep (Stemcell Technologies) and density gradient centrifugation. CD8 T cells were depleted by positive selection using the EasySep™ Human CD8 Positive Selection Kit (Stemcell Technologies) and EasySep™ magnet (Stemcell Technologies). Depletion of CD8 T cells was confirmed by staining an aliquot of 50,000 CD8 depleted PBMC with anti-CD3-FITC (clone: OKT3), anti-CD4-PE (clone: OKT4) and anti-CD8-APC (clone: HIT8a) fluorescent antibodies and analysis with an Accuri C6 flow cytometer and software (BD Biosciences).
Antigen reactivity of CD8 T cell depleted PBMC to the peptide pools was determined where indicated by IFN-γ ELISpot assay (see below), and cells were subsequently stimulated with the various antigen peptide pools (see below) at 1 µg/ml each peptide. After 48 hours of antigen stimulation, an aliquot of ~ 50,000 CD8 depleted PBMC of each condition was stained for anti-CD4-APC (clone: OKT4) and anti-CD69-PE (clone: FN50) and analyzed using the Accuri C6 flow cytometer and software (BD Biosciences). All antibodies for flow cytometry were obtained from BioLegend.

Vollbrecht T., Angerstein A.O., Menke B., Kumar N.M., de Oliveira M.F., Richman D.D, & Guatelli J.C. (2020). Inconsistent reversal of HIV-1 latency ex vivo by antigens of HIV-1, CMV, and other infectious agents. Retrovirology, 17, 36.

+ Open protocol

+ Expand

Exosome Surface Marker Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies to CD9 (HI9a), CD63 (NVG-2), CD63 (H5C6), CD81 (Eat-2), CCR9 (9B1), CXCR4 (L276F12), and CX3CR1 (SA011F11) were purchased from BioLegend (San Diego, CA). Isotype controls including Rat IgG2a, Rat IgG2b, Armenian Hamster IgG, Mouse IgG2a, and Mouse IgG1 were also from BioLegend. The antibody to CD9 (KMC8) was obtained from BD Biosciences. The antibodies to CCR7 (4B12), CCR10 (248918) were acquired from R&D Systems. The cells or microbead-conjugated exosomes were stained with the fluorescently labeled antibodies, washed twice with PBS containing 2% FBS and 2 mM ethylenediaminetetraacetic acid (EDTA) (Wako, Osaka, Japan), and analyzed by using BD Accuri C6 flow cytometer and software (BD Biosciences). For this method of microbead conjugation of exosomes, only positive events can be detected and fluorescently quantified in the flow cytometry. In some experiments, total (intracellular plus surface) staining experiment was done by using FIX and PERM Kit (Thermo Fisher Scientific) according to manufacturer's instructions.

Park E.J., Myint P.K., Appiah M.G., Worawattananutai P., Inprasit J., Prajuabjinda O., Soe Z.Y., Gaowa A., Kawamoto E, & Shimaoka M. (2021). Ligand-competent fractalkine receptor is expressed on exosomes. Biochemistry and Biophysics Reports, 26, 100932.

+ Open protocol

+ Expand

Cytokine Profiling of CAR T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

1x10⁵ TZ47 CAR only or Super2 + IL4, IL5, IL25, IL33 or thymic stromal lymphopoietin (TSLP) T cells were co-cultured with recombinant B7H6 (Biolegend 794006) or B16F10-B7H6 tumor cells for 24 hrs at 37°C. Tumor cell lines were plated at a 1:1 and 0.5:1 Effector:Target ratio. Cell-free medium was collected and analyzed for IL4, IL5, IL25, IL33 and TSLP using ELISAs or LEGENDplex™ assays (Biolegend, 740055, 740056, 447104, 434104; Invitrogen 88-7333-22) as described in the manufacturers’ protocols using an Epoch BioTek plate reader with Gen5 1.11 2005 software or BD Accuri C6 Flow Cytometer and Software.

Brog R.A., Ferry S.L., Schiebout C.T., Messier C.M., Cook W.J., Abdullah L., Zou J., Kumar P., Sentman C.L., Frost H.R, & Huang Y.H. (2022). Superkine IL2 and IL33 armored CAR T cells reshape the tumor microenvironment and reduce growth of multiple solid tumors. Cancer immunology research, 10(8), 962-977.

+ Open protocol

+ Expand

Comprehensive Blood Cell Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

For white blood cell analysis, mixed blood was collected from heart puncture into EDTA‐treated vacuum tubes (BD biosciences, Franklin Lakes, NJ). Erythrocytes were lysed in hypotonic ammonium‐chloride buffer for 10 min with gentle agitation at 4°C, and then, centrifuged at 250× g for 10 min at 4°C. The supernatant was removed, and pellet was washed twice with cold PBS. The pellet was then resuspended in PBS containing 5 mM of EDTA and 0.5% (w/v) bovine serum albumin (FACS buffer). Purified white blood cells were probed 30 min with the following antibodies: Anti‐CD45‐APC (Cell Signaling Technologies, Danvers, MA), anti‐Ly6G‐FITC (Sigma‐Aldrich, St. Louis, MO), and anti‐CD64‐PE (Thermo‐Fisher). Samples were washed twice with FACS buffer, resuspended in 300 μl of FACS buffer and analyzed immediately.
For platelet analysis, 200 µl of whole blood was collected from the inferior vena cava and added to tubes containing 40 ul of acid‐citrate‐dextrose buffer at 30°C and a portion of blood was aliquoted for counting. Blood was diluted 100x in cold FACS buffer and mixed gently. Diluted whole blood was probed with anti‐CD9‐APC (Thermo‐Fisher) for 30 min. Samples were washed twice with FACS buffer, resuspended in 300 μl of FACS buffer and analyzed immediately.
The Accuri C6 flow cytometer and software (BD Biosciences) was used for all data acquisition and analysis.

Eudy B.J., McDermott C.E., Liu X, & da Silva R.P. (2020). Targeted and untargeted metabolomics provide insight into the consequences of glycine‐N‐methyltransferase deficiency including the novel finding of defective immune function. Physiological Reports, 8(18), e14576.

+ Open protocol

+ Expand

Quantifying Edited Hematopoietic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Between 4 and 8 days post targeting with fluorescent gene replacement vectors, CD34⁺ HSPCs were harvested and the percentage of edited cells was determined by flow cytometry. Cells were analyzed for viability using Ghost Dye Red 780 (Tonbo Biosciences), and reporter expression was assessed using either the Accuri C6 flow cytometer and software (v.9.4.11; BD Biosciences) or the FACS Aria II cytometer and FACS Diva software (v.8.0.3; BD Biosciences). The data were subsequently analyzed using FlowJo (v.10.6.1; FlowJo LLC).

Cromer M.K., Camarena J., Martin R.M., Lesch B.J., Vakulskas C.A., Bode N.M., Kurgan G., Collingwood M.A., Rettig G.R., Behlke M.A., Lemgart V.T., Zhang Y., Goyal A., Zhao F., Ponce E., Srifa W., Bak R.O., Uchida N., Majeti R., Sheehan V.A., Tisdale J.F., Dever D.P, & Porteus M.H. (2021). Gene replacement of α-globin with β-globin restores hemoglobin balance in β-thalassemia-derived hematopoietic stem and progenitor cells. Nature medicine, 27(4), 677-687.

+ Open protocol

+ Expand

Mitochondrial Depolarization Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

The percentage mitochondrial depolarisation (Δψ m ) was measured using FACS and the JC-1 Mitoscreen (BD Biosciences, San Jose, CA). Briefly, JC-1 stock solution was diluted in 1X Assay Buffer (37°C) to make up a working solution. Approximately 200,000 cells suspended in 100 μl of 0.1 M PBS from each treatment were transferred to 1.5 ml tubes containing 100 μl of JC-1 staining solution and incubated (10 min, 37°C). Thereafter, 100 μl of FACS sheath fluid was added to each sample. Flow cytometry data from stained cells (50,000 events) were captured with the Accuri™ C6 flow cytometer and software (BD Biosciences). Live cells were gated using CFlow Plus Software (BD Biosciences).

Nagiah S., Phulukdaree A, & Chuturgoon A. (2015). Mitochondrial and Oxidative Stress Response in HepG2 Cells Following Acute and Prolonged Exposure to Antiretroviral Drugs. Journal of cellular biochemistry, 116(9).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!