For immunoblotting analyses, 20E2 cells were lysed in RIPA lysis buffer supplemented with protease and phosphatase inhibitors (Roche Applied Science). Immunoblotting was performed as described previously74 (link). Primary antibodies used are: anti-myc tag mAb (Cell Signaling, Danvers, MA), C20 antibody, anti-β actin mAb (Sigma-Aldrich), anti-Phospho-Tyrosine mAb (Cell Signaling, Danvers, MA). Detection was performed with the Li-Cor Odyssey imaging system and quantitated with ImageJ software.
Anti myc tag mab
Manufactured by Cell Signaling Technology
Anti-myc tag mAb is a monoclonal antibody that specifically recognizes the c-myc epitope tag. It is a commonly used tool for the detection and immunoprecipitation of proteins tagged with the c-myc peptide sequence.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin mab, by Merck Group (1 mentions)
Odyssey imaging system, by LI COR (1 mentions)
Ripa lysis buffer, by Roche (1 mentions)
Protease and phosphatase inhibitor, by Roche (1 mentions)
Coralite488 conjugated affinipure goat anti rabbit igg, by Proteintech (1 mentions)
Lsm 880 fluorescent microscope, by Zeiss (1 mentions)
2 protocols using anti myc tag mab
1
Immunoblotting Analysis of Protein Phosphorylation
2
Immunofluorescence Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunofluorescence was performed as previously described75 (link). Primary antibodies used were C20 antibody and anti-myc tag mAb (Cell Signaling, Danvers, MA). Secondary antibodies were CoraLite488-conjugated Affinipure Goat Anti-Rabbit IgG and CoraLite594-conjugated Affinipure Goat Anti-Mouse IgG (proteintech, Wuhan, China). Images were captured by LSM 880 fluorescent microscope (Carl Zeiss, Jena, Germany) and analyzed with ZEN software.
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