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50 protocols using ph meter

1

Cuttlefish pH Measurement Protocol

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pH value was extracted according to the method of Song et al. [18 (link)]. Measured using a pH meter (Sartorius, Gottingen, Germany). Dilute 2 g of cuttlefish with distilled water to form a 20 mL homogenate. Use a pH meter (Sartorius, Gottingen, Germany) to measure the pH.
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2

Ocular Fibrosis Treatment Assay

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The assays include chloroform (TCM), dimethylformamide (DMF), dimethyl sulfoxide (DMSO) and PHBV, which were purchased from Sigma (USA). Rosiglitazone was obtained from BioVision (USA). Ammonium formate came from Fisher scientific (USA). Acetonitrile was obtained from Merck Millipore (Germany). Filed emission scanning electron microscope SU8010 was obtained from Hitachi (Tokyo, Japan). LC-20A high-performance liquid chromatography (HPLC) were obtained from Shimadzu equipped with an autosampler (Model SIL-20A), a column temperature controller compartment (CTO-10AS) and ultraviolet detector (SPD-M20A). Diamonsil C18 column (5 μ 250 × 4.6 mm) were purchased from Dikma Technologies Inc., China. PH meter came from Denver Instrument, USA. TonoVet were obtain from Icare, Finland. In the vivo experiments, the assays include pentobarbital sodium (30 mg/kg) (Solarbio, Beijing, China), Benoxil (Santen, Japan), Tobramycin Dexamethasone Eye Ointment (Alcon, USA), 0.5% Levofloxacin Eye Drops (Santen, Japan), 4% Paraformaldehyde (Solarbio, Beijing, China), suture line (10-0 nylon; Alcon; USA), 8-0 Vicryl suture (Ethicon; USA), Normal Saline (Second Xiangya Hospital, Changsha, China), Mitomycin C (Sigma, USA), anti-Collagen I antibody (Abcam, UK), anti-α-SMA antibody (Abcam, UK), and anti-connective tissue growth factor (CTGF) antibody (Abcam, UK).
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3

Determination of Maize pH

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The pH of each sample was determined using a pH meter (Denver Instrument Company, Goettingen, Germany). Seventy grams of distilled water were added to seventy grams of maize that had been placed in a 500 mL beaker. A magnetic mixer was used to stir the contents of the beaker for 50 s, and then the liquid was poured into a clean 15 mL centrifuge tube. The pH reading was recorded after 30 s. All measurements were taken in triplicate.
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4

pH-Dependent Particle Uptake and Viability

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For evaluation of pH-specificity of particle uptake and cell viability of tumor cells, pH specific media was utilized. Briefly, phosphate buffer (25 mM) at the desired pH (7.4, 6.6) was prepared by dissolving sodium phosphate monobasic and dibasic solution dissolved in distilled water (Sigma-Aldrich, St. Louis, MO, USA). 13.6 g of DMEM powder was dissolved in the autoclaved phosphate buffer (25mM) at the desired pH (7.4 or 6.6). The solution was enriched with 10% FBS and 1% L-glutamine and filtered through grade-1 Whatman qualitative filter paper (Sigma-Aldrich, St. Louis, MO, USA). The pH of the solution was determined by pH meter (Denver Instrument Ultrabasic, Bohemia, NY, USA). The acidity and basicity adjustments of the pH solutions were performed with sterilized sodium hydroxide (1 M) or hydrochloric acid (1 M).
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5

pH Measurement of Bacterial Growth Media

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The pH of the growth media was measured as follows. Before bacterial inoculation, the pH of 1:20 TSB with or without 0.2% or 2% L-arabinose was measured by a pH Meter (Denver Instrument). O/N cultures were normalized to OD600 = 0.8, diluted 1:10 into 20 mL 1:20 TSB, with or without 0.2% or 2% L-arabinose added, in 50 mL screw-cap conical centrifuge tubes (CellPro). The tubes were incubated for 24 hr at 25°C on a GyroMini nutating mixer (LabNet International, Inc.) at 24 rpm until the bacterial culture reached logarithmic growth. Cultures were then pelleted, the supernatant removed to a new 50 mL tube, and the pH was measured.
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6

pH Measurement of Drug Agents

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pH of the drug agents used was measured using a pH meter (Denver instrument GmbH, Germany). Each measurement was in triplicates and the mean ±SD (n=3) computed and recorded.
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7

Honey Viscosity and pH Analysis

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Viscosity was measured using a rotational viscometer (Brookfield viscometer DV-III+ Rheometer, USA) with SC4-29 spindle. The pH of honey was also measured by pH meter (Denver instrument, USA).
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8

Dissolution Study and In Vitro Drug Release Evaluation

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A dissolution study of the prepared patches and evaluation of in vitro drug release was conducted in accordance with the method described in the USP [23 ]. A pharma test dissolution apparatus (Pharma test PTWS 820D, Hainburg, Germany) was used. The patches were kept at the bottom of vessels containing a dissolution medium. The studies were carried out at 32 ± 0.5 °C at 50 rpm [24 (link)]. All the vessels were covered with lids, and at specific time intervals of 0, 0.05, 1, 1.5, 2, 4, 6, 8, 12, 20, and 24 h, 5 mL of samples were collected from the dissolution medium, which was simultaneously replaced with an equal volume of fresh dissolution medium. Spectrophotometric analysis of the drug in the dissolution medium at the respective wavelength (545 nm) was carried out using phosphate buffer at pH 7.4 (pH Meter, Denver Instrument, 5 Orville Dr, Bohemia, NY 11716, United States) as a blank.
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9

Potentiometric Titration of Cat-CH

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Cat-CH was dissolved in water or 50 mM HCl at 0.1% (w/v) and titrated with 0.1M, 0.5M or 2M NaOH depending on the required precision. The pH was monitored with a Denver Instrument pH meter. pKa was assessed at half-equivalence with Eq.( 2) (neq being the amount of NaOH at equivalence).
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10

Electrochemical Characterization of Ascorbic Acid

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Electroanalytical digital balance (Nimbus, UK), pH meter (Denver instrument, Hungary), orbital shaker, CHI760D Electrochemical Workstation (Austin, USA) connected to a personal computer were used. All electrochemical experiments were performed employing a conventional three-electrode system with a carbon paste electrode as a working electrode, platinum coil as a counter electrode and Ag/AgCl as a reference electrode. Sodium hydroxide, hydrochloric acid, sodium phosphate monobasic, sodium phosphate dibasic, pH standard solutions (pH 4, pH 7, and pH 10), ascorbic acid (all from Blulux chemical Ltd, India) were used. All reagents were of analytical grade and hence were used directly without further purification.
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