M200 pro readers

Manufactured by Tecan

The M200 Pro readers are versatile microplate readers designed for a wide range of absorbance-based applications in life science research and clinical laboratories. The readers offer high-performance detection capabilities and can measure absorbance across a broad wavelength range.

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Lab products found in correlation

Luciferin, by GoldBio (2 mentions) Dexamethasone (dex), by Thermo Fisher Scientific (2 mentions) White 96 well plate, by Greiner (2 mentions) Admem, by Thermo Fisher Scientific (2 mentions) Multicycle, by Actimetrics (2 mentions)

2 protocols using m200 pro readers

Circadian Rhythm Assay in Fibroblasts

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Fibroblasts were cultured to confluence (~30,000 cells/well) in 96-well white plates (Greiner Bio-One, Gloucestershire, UK) using ADMEM (ThermoScientific) containing 10% FBS and 1% penicillin–streptomycin. Cells were synchronized with 200 nM dexamethasone, diluted in serum-free medium for an hour and washed twice with the same solution (ThermoScientific) before reconstituting with serum-free medium containing 1× B27 (ThermoScientific) and 400 μM luciferin (Gold Biotechnology, St. Louis, Missouri, USA). Bioluminescence was recorded for 4 days in Tecan M200 Pro readers. Actimetrics MultiCycle was used to determine the period and amplitude (baseline subtracted 24 h running average of the raw luminescence data smoothed over 8 h). For luminometric experiments involving the 62 fibroblast cell lines, three technical replicates per dose, per drug, were conducted once and averaged.

Sanghani H.R., Jagannath A., Humberstone T., Ebrahimjee F., Thomas J.M., Churchill G.C., Cipriani A., Attenburrow M.J., Perestenko O.V., Cowley S.A., Cader M.Z., Peirson S.N., Harrison P.J., Foster R.G., Goodwin G.M, & Vasudevan S.R. (2020). Patient fibroblast circadian rhythms predict lithium sensitivity in bipolar disorder. Molecular Psychiatry, 26(9), 5252-5265.

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Circadian Rhythm Profiling in Fibroblasts

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Fibroblasts were cultured to confluence (~30 000 cells/well) in 96-well white plates (Greiner Bio-One, Gloucestershire, UK) using ADMEM (ThermoScientific) containing 10% FBS and 1% penicillin-streptomycin. Cells were synchronized with 200nM dexamethasone, diluted in serum-free medium for an hour and washed twice with the same solution (ThermoScientific) before reconstituting with serum-free medium containing 1x B27 (ThermoScientific) and 400μM luciferin (Gold Biotechnology, St. Louis, Missouri, USA). Bioluminescence was recorded for 4 days in Tecan M200 Pro readers. Actimetrics MultiCycle was used to determine the period and amplitude (baseline subtracted 24h running average of the raw luminescence data smoothed over 8h). For luminometric experiments involving the 62 fibroblast cell lines, three technical replicates per dose, per drug, were conducted once and averaged.

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