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2 plex procartaplex

Manufactured by Thermo Fisher Scientific
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The 2-Plex ProcartaPlex is a multiplexed immunoassay panel that allows for the simultaneous quantification of two analytes in a single well. It is designed for use with the Luminex platform, enabling efficient and sensitive detection of target proteins.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions) Luminex 100, by Bio-Rad (2 mentions) Bcg bromocresol green albumin assay kit, by Merck Group (2 mentions) Procartaplex cytokine and chemokine mouse 36 plex, by Thermo Fisher Scientific (2 mentions) Elisa, by R&D Systems (2 mentions)

2 protocols using 2 plex procartaplex

1

Comprehensive Lung Inflammation Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were euthanized (500 mg/kg Ketamine + 50 mg/kg Xylazine) and BALF was collected through catheter insertion intratracheally, injecting and retracting 1 ml of ice-cold PBS. BALF was centrifuged at 300 × g for 5 min at 4°C and supernatants were stored at −80°C. Pelleted cells in the BALF were counted and analysed by flow cytometry to determine the frequency of Ter119+ RBCs on day 6 post infection unless otherwise indicated. Frequencies of immune cell populations were quantified by flow cytometry. Concentrations of IFN-α/β were measured using 2-Plex ProcartaPlex (Invitrogen). IFN-λ was measured by ELISA (R&D). Total protein concentrations in BALF were determined using the Pierce BCA Protein Assay Kit (Thermo Scientific) according to manufacturer’s instructions. Serum albumin was quantified using a BCG (Bromocresol Green) Albumin Assay Kit (Sigma) according to manufacturer’s instructions. The ProcartaPlex Cytokine and Chemokine Mouse 36-Plex (eBioscience) was used to assess cytokine concentrations in BALF read on a Luminex 100 (BioRad).
Major J., Crotta S., Finsterbusch K., Chakravarty P., Shah K., Frederico B., D’Antuono R., Green M., Meader L., Suarez-Bonnet A., Priestnall S., Stockinger B, & Wack A. (2023). Endothelial AHR activity prevents lung barrier disruption in viral infection. Nature, 621(7980), 813-820.
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2

Comprehensive Lung Inflammation Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were euthanized (500 mg/kg Ketamine + 50 mg/kg Xylazine) and BALF was collected through catheter insertion intratracheally, injecting and retracting 1 ml of ice-cold PBS. BALF was centrifuged at 300 × g for 5 min at 4°C and supernatants were stored at −80°C. Pelleted cells in the BALF were counted and analysed by flow cytometry to determine the frequency of Ter119+ RBCs on day 6 post infection unless otherwise indicated. Frequencies of immune cell populations were quantified by flow cytometry. Concentrations of IFN-α/β were measured using 2-Plex ProcartaPlex (Invitrogen). IFN-λ was measured by ELISA (R&D). Total protein concentrations in BALF were determined using the Pierce BCA Protein Assay Kit (Thermo Scientific) according to manufacturer’s instructions. Serum albumin was quantified using a BCG (Bromocresol Green) Albumin Assay Kit (Sigma) according to manufacturer’s instructions. The ProcartaPlex Cytokine and Chemokine Mouse 36-Plex (eBioscience) was used to assess cytokine concentrations in BALF read on a Luminex 100 (BioRad).
Major J., Crotta S., Finsterbusch K., Chakravarty P., Shah K., Frederico B., D’Antuono R., Green M., Meader L., Suarez-Bonnet A., Priestnall S., Stockinger B, & Wack A. (2023). Endothelial AHR activity prevents lung barrier disruption in viral infection. Nature, 621(7980), 813-820.
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