Ffa quantification assay kit

Manufactured by Abcam

Sourced in China, United Kingdom

The FFA quantification assay kit is a laboratory tool used to measure the concentration of free fatty acids in various samples. The kit provides the necessary reagents and protocols to perform this analysis.

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Lab products found in correlation

Cobas c system, by Hitachi (1 mentions)

Close spelling variants of this product

FFA quantification kit FFA assay kit Quantification kits

4 protocols using ffa quantification assay kit

Quantifying Tissue Lipid Profiles

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Tissue TG and FFA contents were obtained and measured according to the instructions of a TG quantification assay kit (Cat# E1003, Applygen, Beijing, China) and FFA quantification assay kit (Cat# ab65341, Abcam, Cambridge, Cambridgeshire, United Kingdom), respectively.

Li L., Zhang X., Zhang Q., Jia J., Zhang J., Zhang D., Song H., Chen B., Hu J, & Huang Y. (2019). Myocardial Adipose Triglyceride Lipase Overexpression Protects against Burn-Induced Cardiac Lipid Accumulation and Injury. Oxidative Medicine and Cellular Longevity, 2019, 6428924.

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Fasting Lipid Profile Determination

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Fasted plasma triglycerides and total cholesterol were determined in the Ziekenhuis Oost–Limburg (Genk, Belgium) using Roche/Hitachi cobas c systems (Rotkreuz, Switzerland). Quantification of fasted plasma free fatty acids (FFA) was assessed using an FFA quantification assay kit (Abcam, ab65341, Cambridge, UK) according to the manufacturer’s instructions.

D’Haese S., Verboven M., Evens L., Deluyker D., Lambrichts I., Eijnde B., Hansen D, & Bito V. (2023). Moderate- and High-Intensity Endurance Training Alleviate Diabetes-Induced Cardiac Dysfunction in Rats. Nutrients, 15(18), 3950.

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Lipid Profile Analysis Protocol

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Cholesterol and triglyceride levels in serum were measured using kits from Thermo Scientific. Serum total free fatty Acids (FFA) was measured by using a FFA quantification assay kit from Abcam, Cambridge MA.

Zou J., Chassaing B., Singh V., Pellizzon M., Ricci M., Fythe M., Kumar M.V, & Gewirtz A.T. (2017). Fiber-mediated nourishment of gut microbiota protects against diet-induced obesity by restoring IL-22-mediated colonic health. Cell host & microbe, 23(1), 41-53.e4.

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Colorimetric FFA Quantification

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Total serum free fatty acid (FFA) level was obtained using the FFA Quantification Assay Kit (Abcam). According to the manufacturer's protocol, FFA were converted to their CoA derivatives and oxidized with the generation of color that was read at λ = 570 nm.

Arriga R., Pacifici F., Capuani B., Coppola A., Orlandi A., Scioli M.G., Pastore D., Andreadi A., Sbraccia P., Tesauro M., Daniele N.D., Sconocchia G., Donadel G., Bellia A., Della-Morte D, & Lauro D. (2019). Peroxiredoxin 6 Is a Key Antioxidant Enzyme in Modulating the Link between Glycemic and Lipogenic Metabolism. Oxidative Medicine and Cellular Longevity, 2019, 9685607.

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