Raw264 and HEK293 cells were cultured with DMEM medium containing 10 % fetal bovine serum (FBS) and antibiotics at 37°C in 5 % CO2 incubator. HepG2 cells were cultured in EMEM medium containing 10 % FBS and antibiotics. V5 antibody was from Life technologies (Grand Island, NY). Phospho-PKCδ and cleaved caspase 3 antibodies were from Cell Signaling (Danvers, MA). LPS, ki16425, and β-actin antibody were from Sigma (St. Louis, MO). Immunobilized protein A/G beads and phospho-p38 MAPK antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). All materials in highest grades used in the experiments are commercially available.
Phospho p38 mapk antibodies
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Phospho-p38 MAPK antibodies are immunological reagents that detect the phosphorylated form of the p38 Mitogen-Activated Protein Kinase (MAPK) protein. p38 MAPK is a serine/threonine protein kinase that plays a key role in various cellular processes, including inflammation, apoptosis, and cell differentiation.
Automatically generated - may contain errors
Lab products found in correlation
V5 antibody, by Thermo Fisher Scientific (1 mentions)
Ki16425, by Merck Group (1 mentions)
Immunobilized protein a g beads, by Santa Cruz Biotechnology (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Anti β actin antibody, by Santa Cruz Biotechnology (1 mentions)
2 protocols using phospho p38 mapk antibodies
1
Comparative Analysis of Cell Lines
2
Western Blot and Histological Analysis
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Western blot analysis was performed as previously described[11 (link),12 (link)]. Briefly, 40 μg of protein from total tissue or cell lysate were used, and the blots were probed using polyclonal anti-mouse SphK1, C5aR, p38-MAPK and phospho-p38-MAPK antibodies (Santa Cruz Biotechnology, Dallas, TX, United States), with β-actin detected with anti-β-actin antibody (Santa Cruz Biotechnology) as a loading control. For histological study, liver tissue was fixed in 40 g/L phosphate-buffered formalin, and 3-5 μm tissue sections were cut and stained with hematoxylin and eosin (HE) before microscopic evaluation at × 200 or × 400 magnification.
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