Racemic MDMA hydrochloride was purchased from Lipomed, A.G. (Arlesheim, Switzerland), dissolved in 0.9% physiological saline in order to obtain a dose of 20 mg/kg (2 mg/ml) expressed as the salt, and injected in a volume of 0.1 ml/10 g body weight by intraperitoneal (i.p.) route of administration. Ethyl alcohol was purchased from Merck Chemicals (Darmstadt, Germany) and diluted in tap water in order to obtain a 20% (v/v) ethanol solution. Sodium dodecylsulphate, polyacrylamide gel and poly vinylidene difluoride (PVDF) membrane were obtained from Bio-Rad Laboratory (Teknovas, Bilbao, Spain). Reagents: proteases inhibitor (Boehringer Mannheim, Germany); phosphatase inhibitor cocktail set (Cabiochem, German). High-performance liquid chromatography (HPLC) reagents were purchased from Sigma Aldrich (San Luis, MO, USA).
Hplc reagents
Manufactured by Merck Group
Sourced in United Kingdom, United States
HPLC reagents are high-performance liquid chromatography (HPLC) solvents and additives used in analytical and preparative HPLC systems. These reagents are designed to facilitate the separation, identification, and quantification of various chemical compounds in complex mixtures.
Automatically generated - may contain errors
Lab products found in correlation
Polyacrylamide gel, by Bio-Rad (1 mentions)
Ethyl alcohol, by Merck Group (1 mentions)
Racemic mdma hydrochloride, by Lipomed (1 mentions)
High performance liquid chromatography, by Merck Group (1 mentions)
Sodium dodecyl sulphate (sds), by Bio-Rad (1 mentions)
Proteases inhibitor, by Boehringer Ingelheim (1 mentions)
Polyvinylidene difluoride pvdf membrane, by Bio-Rad (1 mentions)
Diclofenac sodium, by Merck Group (1 mentions)
Voltaren, by Novartis (1 mentions)
Disodium hydrogen phosphate, by Thermo Fisher Scientific (1 mentions)
Methanol, by Avantor (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Potassium dihydrogen phosphate, by Thermo Fisher Scientific (1 mentions)
Lidocaine, by Merck Group (1 mentions)
Nicotine, by Merck Group (1 mentions)
Dustless precision pellets, by Bio-Serv (1 mentions)
Alginic acid sodium salt from brown algae, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Isoquercitrin, by Merck Group (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
8 protocols using hplc reagents
1
MDMA Exposure and Ethanol Effects
2
Comparative Topical Diclofenac Formulations
3
Phosphate Buffer and Nicotine Patch Analysis
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Disodium hydrogen phosphate was purchased from Acros Organics (Geel, Belgium), potassium dihydrogen phosphate from Fisher Scientific (Loughborough, UK), acetonitrile and methanol from VWR Chemicals (Lutterworth, UK). Other solvents, HPLC reagents, lidocaine and (−)-nicotine were obtained from Sigma-Aldrich (Gillingham, UK). Nicotinell® 7 mg/24 h patches (Novartis Consumer Health, Camberley, UK) were bought from Boots UK Limited (Nottingham, UK). Versatis® 5 mg medicated plaster was from AAH Pharmaceuticals Ltd (Coventry, UK). LMX4 (lidocaine 4% w/w cream) was purchased from HI Weldrick Ltd (Doncaster, UK).
4
Rodent Dietary Supplementation Protocol
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Regular chow food (5001 Rodent Diet, 3.0 kcal/g with 29% energy derived from protein, 13% from fat, and 56% from carbohydrate) was obtained from LabDiet. Dustless Precision Pellets (14 mg, 3.6 kcal/g) were from BioServ. Amphetamine hydrobromide, neurochemical standards and HPLC reagents were from Sigma-Aldrich.
5
Betnovate Cream Standardization Protocol
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Betnovate® cream (betamethasone valerate 0.122% w/w) was purchased from Glaxo Wellcome UK Ltd (Uxbridge, UK). All doses of formulation applied in the different components of the study described below were within ± 10% of those targeted. Pure betamethasone 17-valerate, solvents, and HPLC reagents were purchased from Sigma-Aldrich (Gillingham, UK).
6
Alginate-based Growth Media Protocol
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Growth media components were from Merck (Darmstadt, Germany) and Sigma Aldrich (St. Louis, MO, USA). HPLC reagents and alginic acid (sodium salt) from brown algae (with 0.0175 Pa s viscosity at 1% [w/w] and 25 °C) were from Sigma Aldrich (St. Louis, MO, USA). Fluorescent stains were from ThermoFischer Scientific (Waltham, MA, USA). For the complete list of materials, see the Supplementary Materials .
7
Isoquercitrin-Poloxamer 407 Bioreagent Protocol
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Isoquercitrin (quercetin 3-O-glucoside) of 90% purity and poloxamer 407 bioreagent grade [9003-11-6] were purchased from Sigma-Aldrich, while Dulbecco's modified Eagle's medium (DMEM) and Roswell Park Memorial Institute (RPMI) 1640 medium were purchased from Biowest. Fetal bovine serum (FBS), L-glutamax, and antibiotic/antimycotic were purchased from Invitrogen. HPLC reagents, standards, and solvents were of Analytical Reagent (AR) grade and purchased from Sigma-Aldrich. Ferric chloride reagent and Folin–Ciocalteu reagent were purchased from Merck Millipore.
8
Scopolamine Patch Permeability through Pig Skin
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Scopoderm ® 1.5 mg patches (2.5 cm 2 ) (Novartis Consumer Health UK Limited, Camberley, UK) were acquired through the departmental pharmacy (University of Bath, Department of Pharmacy & Pharmacology). Pure scopolamine hydrobromide, solvents and HPLC reagents were from Sigma Aldrich (Gillingham, UK).
Excised abdominal pig skin (from a single animal) was obtained from a local abattoir; to preserve the integrity of the skin barrier, the skin was not exposed to the normal high-temperature cleaning procedure. Skin was washed with water, dermatomed (Zimmer ® , Hudson, OH, USA), to a nominal thickness of 750 µm, frozen within 24 h of slaughter, and thawed before use.
Excised abdominal pig skin (from a single animal) was obtained from a local abattoir; to preserve the integrity of the skin barrier, the skin was not exposed to the normal high-temperature cleaning procedure. Skin was washed with water, dermatomed (Zimmer ® , Hudson, OH, USA), to a nominal thickness of 750 µm, frozen within 24 h of slaughter, and thawed before use.
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