Racemic tocol

Acetonitrile 99.9%, n-hexane 95% and ethyl acetate 99.8% were of HPLC grade, acquired from Fisher Scientific (Lisbon, Portugal). The fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St.
Louis, MO, USA), as also other individual fatty acid isomers, standards of sugars, tocopherols, and organic acids, and trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid). Racemic tocol, 50 mg/mL, was purchased from Matreya (PA, USA).
Micro (Fe, Cu, Mn and Zn) and macroelements (Ca, Mg, Na and K) standards (> 99% purity), as well as LaCl 2 and CsCl (> 99% purity) were purchased from Merck (Darmstadt, Germany). Anthrone was obtained from Panreac (Barcelona, Spain). All other reagents were purchased from specialized retailers. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).

Racemic tocol (50 mg mL -1 ) and tocopherols (α-, β-, γ-, and δ-isoforms) were purchased from Matreya (Pleasant Gap, PA, USA). 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), potassium ferricyanide [K 3 Fe(CN) 6 ], ferric chloride (FeCl 3 ), trichloroacetic acid (TCA), β-carotene, linoleic acid, hydrochloric acid (HCl), sodium hydroxide (NaOH), penicillin/ streptomycin solution (100 U mL -1 and 100 mg mL -1 , respectively), α-glucosidase (isolated from Aspergillus Niger), and 4-p-nitrophenyl-α-D-glucopyranoside (4-pNPG) were purchased from Sigma-Aldrich. Mueller-Hinton agar, nutrient broth and Brain Heart Infusion broth (BHI) were purchased from Biolife (Monza, Italy). NaCl was obtained from CHEMI PHARMA, Tunisia. Hexane, chloroform and methanol were purchased from Merck (Darmstadt, Germany).

Acetonitrile 99.9%, n-hexane 95% and ethyl acetate 99.8% were of HPLC grade from Fisher Scientific (Lisbon, Portugal). Fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St. Louis, MO, USA), as well as other individual fatty acid isomers, L-ascorbic acid, tocopherol, sugar and organic acid standards. Racemic tocol, 50 mg/mL, was purchased from Matreya (Pleasant Gap, PA, USA).

Acetonitrile (99.9%), n-hexane (95%) and ethyl acetate (99.8%) were High Pressure Liquid Chromatography (HPLC) grade from Fisher Scientific (Lisbon, Portugal). The water was obtained from a purification system, Millipore Direct-Q (TGI Pure Water Systems, Greenville, SC, USA). Fatty acid methyl esters standard mixture (standard 47885-U) were purchased from Sigma-Aldrich (St. Louis, MO, USA), as also were other standards: sugars, tocopherols, organic acids and Trolox (6hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid). Phenolic compounds were purchased from Extrasynthèse (Genay, France).
Racemic tocol was purchased from Matreya (Pleasant Gap, PA, USA). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). Foetal bovine serum (FBS), L-glutamine, hank's balanced salt solution (HBSS), trypsin-EDTA (ethylenediaminetetraAcetic acid), penicillin/streptomycin solution (100 U/ml and 100 mg/ml, respectively), RPMI-1640 and DMEM media were from Hyclone (Logan, USA). Acetic acid, ellipticine, sulphorhodamine B (SRB), trypan blue, trichloroAcetic acid (TCA) and Tris were from Sigma Chemical Co. (St Louis, MO, USA). All other chemicals and solvents were of analytical grade and purchased from common sources.

Acetonitrile 99.9%, n-hexane 95% and ethyl acetate 99.8% were of HPLC grade from Fisher Scientific (Lisbon, Portugal). Fatty acid methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St. Louis, MO, USA), as well as other individual fatty acid isomers, L-ascorbic acid, tocopherol, sugar and organic acid standards. Racemic tocol, 50 mg/mL, was purchased from Matreya (Pleasant Gap, PA, USA).

Acetonitrile, n-hexane and ethyl acetate were of HPLC grade from Fisher Scientific (Lisbon, Portugal). Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) and the fatty acid methyl ester (FAME) reference standard mixture 37 (standard 47885-U) were purchased from Sigma (St Louis, MO, USA), as also were L-ascorbic acid, sugar and organic acid standards, acetic acid, formic acid, ellipticine, sulphorhodamine B (SRB), trypan blue, trichloroacetic acid (TCA) and Tris. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). Phenolic compound standards were purchased from Extrasynthèse (Genay, France). Racemic tocol (50 mg mL -1 ) and individual tocopherols were purchased from Matreya (Pleasant Gap, PA, USA). Foetal bovine serum (FBS), L-glutamine, Hank's balanced salt solution (HBSS), trypsin-EDTA (ethylenediaminetetraacetic acid), penicillin/ streptomycin solution (100 U mL -1 and 100 mg mL -1 , respectively), RPMI-1640 and DMEM media were from Hyclone (Logan, Utah, USA). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).