Navitoclax was synthesized at AbbVie, Inc. (North Chicago, IL). FDA-approved drug library was purchased from Enzo (Farmingdale, NY). Albendazole, mebendazole, oxibendazole, and oxfendazole were purchased from Sigma (St. Louis, MO). All siRNA pools were purchased from Dharmacon (Lafayette, CO). Noxa antibody was purchased from Abcam. Bim and Mcl-1 antibodies were purchased from Epitomics (Burlingame, CA). Caspase 3 and caspase 9 inhibitors were purchased from SantaCruz Biotechnology (Santa Cruz, CA). All the branched DNA reagents were purchased from Affymetrix (Santa Clara, CA).
Navitoclax
Manufactured by Abbvie
Sourced in Israel
Navitoclax is a laboratory research tool that targets and inhibits the Bcl-2 family of proteins. It is used in scientific research to study apoptosis and cell death pathways.
Automatically generated - may contain errors
Lab products found in correlation
Venetoclax, by Abbvie (3 mentions)
Mebendazole, by Merck Group (1 mentions)
Oxibendazole, by Merck Group (1 mentions)
Oxfendazole, by Merck Group (1 mentions)
Fda approved drug library, by Enzo Life Sciences (1 mentions)
Sirna pool, by Horizon Discovery (1 mentions)
Albendazole, by Merck Group (1 mentions)
A 1155463, by Abbvie (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
A 1210477, by Abbvie (1 mentions)
Abt 737, by Abbott (1 mentions)
Sn 38, by Merck Group (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
A 1331852, by Abbvie (1 mentions)
5 protocols using navitoclax
1
Navitoclax Drug Screening Protocol
2
Synthesis of BCL-XL inhibitors
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BCL-XL inhibitor A-1155463 and navitoclax were synthesized at AbbVie, Inc. (North Chicago, IL). All the siRNAs were purchased from Dharmacon (Lafayette, CO).
3
In Vitro Evaluation of Anti-Leukemic Compounds
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Mouse p185+Arf−/− B-ALL were grown in RPMI (Life Technologies, CA) with 10% fetal bovine serum, 55 μM 2-mercaptoethanol, 2 mM glutamine, penicillin, and streptomycin. Navitoclax (ABT-263), venetoclax (ABT-199), A-1155463, and A-1210477 were kindly provided by AbbVie, IL. EU-5346 (also known as Compound #9) was provided by Eutropic Pharmaceutical, MA [30 (link)]. UM-36 was kindly provided by Dr. Nikolovska-Coleska [28 (link), 29 (link)]. All compounds were solubilized in DMSO and added at indicated concentrations for cell culture assays.
4
Chemotherapeutic agents and immunotherapy
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ABT-737: The mice were treated with either 100 mg/kg/d ABT-737 solution or an equal volume of carrier solution administrated via intraperitoneal injections (i.p.) for 21 consecutive days. ABT-737 was received from Abbott (now AbbVie Inc). After the treatment the mice were followed up for 21 days. Navitoclax, venetoclax, metformin: The mice were treated with vehicle (5% EtOH, 20% Phosal PG in MQ), 100 mg/kg/d Navitoclax or venetoclax, 300 or 600 g/kg/d metformin or with drug combinations delivered via intragastric (i.g.) route for 21 days. The cohorts were followed up until 60 days after transplantation. Navitoclax and venetoclax were from AbbVie Inc. Drugs were sonicated daily for better solubility. In the experiments including anti-PD-1, mice received i.p. injections of either 200 µg control IgG (bxcell, #BE0089) or 200 µg anti-PD-1 (bxcell, #BE0146) every third day, in total four times, together with the 1-week daily adjuvant i.g. treatments of either vehicle, venetoclax, metformin or the combination of venetoclax+metformin. Paclitaxel was administrated every third day, i.p., 10 mg/kg in cremphor EL-ethanol-saline. Plasma ALAT levels were measured in the Biochemical Analysis Core for Experimental Research, University of Helsinki.
5
Cell Line Authentication and Drug Screening
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Cell lines were purchased from DSMZ, ATCC, ECACC, JCRB Cell Bank or from the KCLB and cultivated for 1-15 passages in the recommended culture media supplemented with 20 mmol/L HEPES (Gibco), penicillin/streptomycin and 10% FBS (Invitrogen; Supplementary Tables S1 andS2). Cells were grown at 37 C in a humidified atmosphere with 5% CO 2 . Cell lines were tested for authenticity by short tandem repeat profiling and Mycoplasma by the AbbVie Core Cell Line Facility. ABBV-621, anti-DR5 agonistic mAb with or without mutant Fc (DANA or LALA), anti-ABBV-621 mAb, venetoclax, navitoclax, A-1331852, paclitaxel, docetaxel, erlotinib and 5-fluorouracil (5-FU) were all synthesized by AbbVie unless otherwise stated. SN38 was purchase from Sigma-Aldrich.
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