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Sertraline

Manufactured by Merck Group
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Sertraline is a laboratory equipment product manufactured by Merck Group. It is a chemical compound used in scientific research and analysis applications. The core function of Sertraline is to serve as a reference standard for analytical testing and validation procedures.

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Lab products found in correlation

Fluoxetine, by Merck Group (10 mentions) Paroxetine, by Merck Group (8 mentions) Citalopram, by Merck Group (7 mentions) Fluvoxamine, by Merck Group (6 mentions) Escitalopram, by Merck Group (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) Serotonin, by Merck Group (3 mentions) Itraconazole, by Merck Group (2 mentions) Cytochalasin d, by Merck Group (2 mentions) Imipramine, by Merck Group (2 mentions) Venlafaxine, by Merck Group (2 mentions) Aprepitant, by Selleck Chemicals (2 mentions) Ritonavir, by Merck Group (2 mentions) Auranofin, by Merck Group (2 mentions) Disulfiram, by Merck Group (2 mentions) Celecoxib, by Merck Group (2 mentions) Itraconazole, by Abcam (2 mentions) Captopril, by Merck Group (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Sertraline hydrochloride (13 citations)

34 protocols using sertraline

1

Antidepressant-like effects of GRg3

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Both GRg3 and sertraline were obtained from Sigma-Aldrich (USA), dissolved in Dmethyl sulfoxide (DMSO, <0.1%) and prepared in physiological saline. The doses of GRg3 (10, 20 and 40 mg/kg i.g) were selected according to its antidepressant-like effects15 (link). sertraline (15 mg/kg i.g) was prepared as a positive control in the behavioral assessments that based on the previous study24 (link).
Xu J.N., Chen L.F., Su J., Liu Z.L., Chen J., Lin Q.F., Mao W.D, & Shen D. (2018). The anxiolytic-like effects of ginsenoside Rg3 on chronic unpredictable stress in rats. Scientific Reports, 8, 7741.
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2

Pharmacological Effects on Zebrafish Development

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Adult wild type zebrafish were obtained from Carolina Biological Supply and have been housed at Brown University over several generations in multiple 10 and 20 gallon tanks. For breeding purposes, the zebrafish were kept in mixed male and female populations on a 14 hour light/10 hour dark cycle. The fish were fed a combination of Gemma fish food, frozen brine shrimp, and frozen bloodworms. Embryos were collected from the tanks at the beginning of the light cycle (“dawn”) and were immediately transferred to deep Petri dishes (Fisher 08-752-11Z) containing ‘egg water’ (0.06 g of Instant Ocean and 0.25 mg of methylene blue per liter of deionized water). The embryos were then treated with various dilutions of bupropion hydrochloride (Sigma B102), sertraline (Sigma S6319), or duloxetine hydrochloride (Sigma SML0474) and grown in an incubator at 28.5 °C with a 14 hour light/10 hour dark cycle at a density of 60–70 embryos per culture dish.
Richendrfer H, & Creton R. (2017). Cluster analysis profiling of behaviors in zebrafish larvae treated with antidepressants and pesticides. Neurotoxicology and teratology, 69, 54-62.
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3

Lipid Vesicles with Antidepressants

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Paroxetine (PAX) and sertraline (SER) were obtained from Sigma Aldrich (USA). 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) in chloroform was purchased from Avanti Polar Lipids (USA) and used without further purification. The stock solution of lipid was made at a concentration of 20 mg mL−1. HEPES (4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid) buffer was obtained from Sigma Aldrich (USA). All liquid suspensions were prepared with 10 mM HEPES buffer containing 50 mM NaCl, at pH 7.4.
Ngo D.T., Nguyen T.Q., Huynh H.K, & Nguyen T.T. (2020). Thermodynamics of selective serotonin reuptake inhibitors partitioning into 1,2-dioleoyl-sn-glycero-3-phosphocholine bilayers. RSC Advances, 10(64), 39338-39347.
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4

Determining Antimicrobial MIC for APEC_O2

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The minimum inhibitory concentration (MIC) of APEC_O2 for sertraline (Sigma-aldrich, Denmark) and tetracycline (Sigma-aldrich, Denmark), respectively, were determined by serial broth dilutions method [28 (link)].
Kromann S., Kudirkiene E., Li L., Thoefner I., Daldorph E., Christensen J.P., Meng H, & Olsen R.H. (2017). Treatment with high-dose antidepressants severely exacerbates the pathological outcome of experimental Escherichia coli infections in poultry. PLoS ONE, 12(10), e0185914.
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5

Antifungal and Antidepressant Drug Preparation

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Fluconazole (FLC), itraconazole (ITC), voriconazole (VRC), caspofungin (CAS), amphotericin B (AMB) and sertraline (SRT) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). The stock solutions of ITC (100 mg/ml), VRC (100 mg/ml), AMB (100 mg/ml) and SRT (100 mg/ml) were freshly prepared in dimethyl sulfoxide (DMSO, Sigma). FLC (100 mg/ml) and CAS (100 mg/ml) were dissolved in sterile distilled water. After serial dilution, the final concentration of DMSO was below 1%.
Cong L., Liao Y., Yang S, & Yang R. (2016). In Vitro Antifungal Activity of Sertraline and Synergistic Effects in Combination with Antifungal Drugs against Planktonic Forms and Biofilms of Clinical Trichosporon asahii Isolates. PLoS ONE, 11(12), e0167903.
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6

Cytotoxicity Screening of Sertraline and Etoposide

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Sertraline (Sigma-Aldrich, #S6319) and Etoposide (Selleckchem, S1225) were dissolved in DMSO and stored at −20 °C and −80 °C. Hydrogen peroxide was purchased from Sigma-Aldrich (H1009).
Heylen E., Verstraete P., Van Aerschot L., Geeraerts S.L., Venken T., Timcheva K., Nittner D., Verbeeck J., Royaert J., Gijbels M., Uyttebroeck A., Segers H., Lambrechts D., Cools J., De Keersmaecker K, & Kampen K.R. (2023). Transcription factor NKX2–1 drives serine and glycine synthesis addiction in cancer. British Journal of Cancer, 128(10), 1862-1878.
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7

Chronic Stress and SSRI Treatment in Mice

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The chronic stress model used consists in the aleatory, intermittent, and unpredictable exposure to different stressors during 5 weeks in C57BL/6J mice. Briefly, animals were randomly and alternately exposed to one of the following stressors for the time indicated for each assay: restraint in well-ventilated tubes for 6 h (20 (link)), tail suspension for 5 min (21 (link)), forced swimming for 5 min (22 (link)), cold temperature exposure (4°C) for 2 h (23 (link), 24 (link)), and 2 days of continuous overnight illumination (25 (link)).
To analyze the effect of SSRIs, mice were orally given 15 mg/kg/day of fluoxetine (Sigma-Aldrich) (26 (link)) or 20 mg/kg/day of sertraline (Sigma-Aldrich) (27 (link)), in a fresh solution prepared in the drinking water. The preparation of these solutions was performed taking into account the volume of water drunk daily by each mouse (5 ml) to reach the indicated dose.
Di Rosso M.E., Sterle H.A., Cremaschi G.A, & Genaro A.M. (2018). Beneficial Effect of Fluoxetine and Sertraline on Chronic Stress-Induced Tumor Growth and Cell Dissemination in a Mouse Model of Lymphoma: Crucial Role of Antitumor Immunity. Frontiers in Immunology, 9, 1341.
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8

Whole-Cell Recording of Kv1.5 Potassium Channels

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The intracellular pipette solution for whole-cell recordings contained 140 mM KCl, 1 mM CaCl2, 1 mM MgCl2, 10 mM HEPES, and 10 mM EGTA, and was adjusted to pH 7.3 with KOH. The bath solution for whole-cell recordings contained 140 mM NaCl, 5 mM KCl, 1.3 mM CaCl2, 1 mM MgCl2, 20 mM HEPES and 10 mM glucose, and was adjusted to pH 7.3 with NaOH. Sertraline (Sigma Chemical Co., St. Louis, MO, USA) was dissolved in dimethyl sulfoxide (DMSO) at 30 mM and further diluted into the bath solution. Final concentration of DMSO in the bath solution, which was less than 0.1%, had no effect on Kv1.5 currents (Data not shown).
Lee H.M., Hahn S.J, & Choi B.H. (2016). Blockade of Kv1.5 channels by the antidepressant drug sertraline. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 20(2), 193-200.
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9

Pharmacological Modulation of TGF-β Signaling

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Fluoxetine and sertraline were purchased from Sigma–Aldrich (St Louis, MO, USA). SB431542 and ARP-100 were purchased from Tocris (Bristol, UK). All the compounds were dissolved in DMSO at the initial concentration of 10 mM. The final concentration of DMSO applied to the cultures was 0.1%. Serotonin was purchased from Sigma–Aldrich (St Louis, MO, USA) and dissolved in PBS. The neutralizing antibody specific for TGF-β1 was purchased from R&D system (Minneapolis, MN, USA), was reconstituted in sterile PBS and used in neuronal cultures at a final concentrations of 2 μg/ml.
Caraci F., Tascedda F., Merlo S., Benatti C., Spampinato S.F., Munafò A., Leggio G.M., Nicoletti F., Brunello N., Drago F., Sortino M.A, & Copani A. (2016). Fluoxetine Prevents Aβ1-42-Induced Toxicity via a Paracrine Signaling Mediated by Transforming-Growth-Factor-β1. Frontiers in Pharmacology, 7, 389.
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10

MTT Assay for Cell Viability

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3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (thiazol blue; MTT), sodium dodecyl sulfate (SDS), M-199 medium, RPMI-PR-1640 medium (w/o phenol red), and cholesterol were purchased from Sigma–Aldrich (St. Louis, MO, USA). Hydrogenated phospholipids were kindly donated by Lipoid GmbH (Ludwigshafen, Germany). Sertraline and other analytical reagents were purchased from Sigma–Aldrich (St. Louis, MO, USA). Molecular biology reagents are purchased from Life Technologies, and CBA (cytometric beads array) was purchased from BD (San Jose, CA, USA).
Romanelli M.M., da Costa-Silva T.A., Cunha-Junior E., Dias Ferreira D., Guerra J.M., Galisteo AJ J.r., Pinto E.G., Barbosa L.R., Torres-Santos E.C, & Tempone A.G. (2019). Sertraline Delivered in Phosphatidylserine Liposomes Is Effective in an Experimental Model of Visceral Leishmaniasis. Frontiers in Cellular and Infection Microbiology, 9, 353.
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