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Ammonium sulfide solution

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Ammonium sulfide solution is a clear, colorless liquid used in various laboratory applications. It is a source of sulfide ions and is commonly employed in analytical chemistry, materials science, and biomedical research. The solution's core function is to provide a controlled source of sulfide ions for precipitation reactions, complexation studies, and other chemical processes that require the presence of sulfide species.

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Lab products found in correlation

Sodium hydroxide (naoh), by Merck Group (3 mentions) Hydrazine hydrate, by Merck Group (3 mentions) Iron 2 chloride tetrahydrate, by Merck Group (2 mentions) Nitric acid, by Merck Group (2 mentions) Glutaraldehyde solution, by Merck Group (2 mentions) Ethanol, by Avantor (2 mentions) Polyvinylpyrrolidone, by Merck Group (2 mentions) Diethylene glycol, by Merck Group (2 mentions) N methyldiethanolamine, by Merck Group (2 mentions) Sodium cacodylate trihydrate, by Merck Group (2 mentions) Iron 3 chloride hexahydrate, by Avantor (2 mentions) Copper 2 nitrate hemi pentahydrate, by Merck Group (2 mentions) Hematoxylin, by Merck Group (1 mentions) Potassium ferricyanide, by Merck Group (1 mentions) Potassium ferrocyanide, by Merck Group (1 mentions) 3 3 diaminobenzidine (dab), by Vector Laboratories (1 mentions) Oleic acid, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Dimethylformamide, by Merck Group (1 mentions) Formamide, by Merck Group (1 mentions)

Close spelling variants of this product

Ammonium solution (13 citations) Ammonium sulfide (10 citations)

6 protocols using ammonium sulfide solution

1

Turnbull's and Perls' Staining for Iron Detection

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We used Turnbull’s blue staining to detect ferrous
(Fe2+) iron in tissues. Briefly, fixed OHSCs were incubated in
10% ammonium sulfide solution (Sigma) for 90 min, followed by 20%
potassium-ferricyanide (Sigma) with 1% hydrochloric acid for 15 min and methanol
with 0.01 M NaN3 (Sigma) and 0.3% H2O2 (Sigma)
for 60 min (Kroner et al., 2014 (link)).
Sections were washed between incubations with PBS.
To detect ferric iron (Fe3+) in tissues, we used
3,3′-diaminobenzidine (DAB; Vector Laboratories,
Burlingame, CA)-enhanced Perls’ staining as previously described (Li et al., 2017b (link)) with slight modification.
Briefly, fixed OHSCs or sections of brain tissue were washed with PBS and
incubated in freshly prepared Perls’ solution (5% potassium ferrocyanide
[Sigma]/10% hydrochloric acid) for 1 h, followed by five PBS washes. After DAB
incubation for 3 min and hematoxylin (Sigma) counterstaining, iron deposition
was digitized and analyzed with Image J software.
Li Q., Lan X., Han X., Durham F., Wan J., Weiland A., Koehler R.C, & Wang J. (2021). Microglia-derived interleukin-10 accelerates post-intracerebral hemorrhage hematoma clearance by regulating CD36. Brain, behavior, and immunity.
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2

Synthesis of Colloidal Nanocrystals

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Indium(III) acetate (In(OAc)3,
99.99%), zinc(II) acetate (Zn(OAc)2, 99.99%), palmitic
acid (PA, C15H31COOH, 99.99%, stored at
−20 °C), gallium(III) chloride (beads, Sigma-Aldrich,
99.99%), tris(trimethylsilyl)-phosphine (P(TMS)3, 95%), 1-octadecene (ODE, 95%), oleic acid (Sigma-Aldrich,
99.99%), ammonium sulfide solution ((NH4)2S,
20% in H2O), 1-octadecene (ODE, 95%), formamide (FA, 99%),
dimethylformamide (DMF, 99%), Zn stearate (technical grade),
sulfur powder (99.98% trace metals basis), and trioctylphosphine
(TOP, 90% technical grade) were purchased from Sigma-Aldrich. Selenium
powder (99.98% trace metals basis) was purchased by Chem Pure. All
the chemicals were used without further purification.
Pietra F., Kirkwood N., De Trizio L., Hoekstra A.W., Kleibergen L., Renaud N., Koole R., Baesjou P., Manna L, & Houtepen A.J. (2017). Ga for Zn Cation Exchange Allows for Highly Luminescent and Photostable InZnP-Based Quantum Dots. Chemistry of Materials, 29(12), 5192-5199.
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3

Synthesis and Characterization of Metal Nanoparticles

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All reagents were of analytical purity and used without further purification. Iron(II) chloride tetrahydrate (FeCl2·4H2O, 99%), sodium hydroxide (NaOH, 99.99%), diethylene glycol (DEG, 99%), N-methyldiethanolamine (NMDEA, 99%), nitric acid (HNO3, 70%), copper(II) nitrate hemi(pentahydrate) (Cu(NO3)2·2.5H2O ≥99.99%), polyvinylpyrrolidone (PVP, Mw 55 kDa), hydrazine hydrate (55%), ammonium sulfide solution ((NH4)2S, 20%), dihydrorhodamine 123 (DHR123 ≥95%), sodium cacodylatetrihydrate (≥98%), glutaraldehyde solution (25% in H2O), hydrogen peroxide solution (30 wt. % in H2O) and formalin solution (10%) were purchased from Sigma-Aldrich (France). Iron(III) chloride hexahydrate (FeCl3·6H2O, 99%) and ethanol were obtained from VWR (France). Alamar BlueTM cell viability reagent was purchased from Thermo-Fisher.
Curcio A., Silva A.K., Cabana S., Espinosa A., Baptiste B., Menguy N., Wilhelm C, & Abou-Hassan A. (2019). Iron Oxide Nanoflowers @ CuS Hybrids for Cancer Tri-Therapy: Interplay of Photothermal Therapy, Magnetic Hyperthermia and Photodynamic Therapy. Theranostics, 9(5), 1288-1302.
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4

Synthesis and Functionalization of Iron Oxide Nanoparticles

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Iron(II) chloride tetrahydrate (FeCl2·4H2O, 99%), sodium hydroxide (NaOH, 99.99%), diethylene glycol (DEG, 99%), N-methyldiethanolamine (NMDEA, 99%), nitric acid (HNO3, 70%), copper(II) nitrate hemi(pentahydrate) (Cu(NO3)2·2.5H2O, ≥99.99%), polyvinylpyrrolidone (PVP, Mw 55 kDa), poly(ethylene glycol) methyl ether thiol (PEG-SH, Mw 2 kDa) hydrazine hydrate (55%), ammonium sulfide solution ((NH4)2S, 20%), glutaraldehyde solution (25% in H2O), and sodium cacodylatetrihydrate (≥98%) were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Iron(III) chloride hexahydrate (FeCl3·6H2O, 99%) and ethanol were obtained from VWR (Rosny-sous-Bois, France).
Curcio A., Van de Walle A., Péchoux C., Abou-Hassan A, & Wilhelm C. (2022). In Vivo Assimilation of CuS, Iron Oxide and Iron Oxide@CuS Nanoparticles in Mice: A 6-Month Follow-Up Study. Pharmaceutics, 14(1), 179.
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5

Synthesis of Chalcogenide Materials

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Antimony(III) sulfide (99.995%. Sigma Aldrich), antimony(III) selenide (99.99%, Sigma Aldrich), tin powder (99.8%, 325 mesh, Acros Organics), selenium powder (99.999%, 200 mesh, Alfa Aesar), sulfur (99.998%, Sigma Aldrich), copper powder (99%, Alfa Aesar), ammonium sulfide solution (40–48 wt% in H2O, Sigma Aldrich), chloroplatinic acid solution (8 wt% in H2O, Sigma Aldrich), ammonium tetrathiomolybdate (99.97%, Sigma Aldrich), 2-[2-(5-methylfuran-2-yl)vinyl]-4,6-bis(trichloromethyl)-1,3,5-triazine (MFVT, TCI), N-(trifluoromethylsulfonyloxy)-1,8-naphthalimide (IM-NIT, WIMAS Corp.), ethylenediamine (≥99.5%, Sigma Aldrich), 1,2-ethanedithiol (≥98.0%, Sigma Aldrich), anhydrous acetonitrile (99.8%, Sigma Aldrich), anhydrous toluene (99.8%, Sigma Aldrich), N-methyl formamide (99%, Sigma Aldrich), anhydrous dimethyl sulfoxide (≥99.9%, Sigma Aldrich), ethyl alcohol (99.5%, Samchun), and methyl alcohol (99.5%, Samchun) were used in the experiments. All chemicals and reagents were used as received without further purification.
Baek S., Ban H.W., Jeong S., Heo S.H., Gu D.H., Choi W., Choo S., Park Y.E., Yoo J., Choi M.K., Lee J, & Son J.S. (2022). Generalised optical printing of photocurable metal chalcogenides. Nature Communications, 13, 5262.
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6

Synthesis of Chalcogenide Thin Films

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Te dioxide (99.9995%), sodium hydroxide (ACS reagent, ≥97.0%, pellets), PVP, average molecular weight (~55,000), ethylene glycol (ReagentPlus®, ≥99%), hydrazine hydrate (78–82%, iodometric), platinum wire (99.9%), ammonium sulfide solution (40–48 wt% in H2O), and methanol (reagent grade, 98%) were purchased from Sigma Aldrich. Acetone (J. T. Baker®), dichloromethane (BDH®), and isopropyl alcohol (ACS Grade) were purchased from VWR International. Sodium sulfide (anhydrous, min. 99.5%), sodium sulfide nonahydrate (ACS reagent, >95%), sodium hydrosulfide hydrate, potassium sulfide (anhydrous, min. 95%), and potassium hydrosulfide (anhydrous, min. 95%) were purchased from Strem Chemicals. 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMIM][TFSI]) was purchased from Solvent Innovation GmbH (Germany). All chemicals were used as delivered without further purification.
Glass substrates, 9.5 × 9.5 mm, and 0.2 mm thick, were purchased from Thin Film Devices. <100>-oriented, boron-doped Si wafers (resistivity = 0.005–0.01 Ω cm, thickness = 525 ± 25 μm) coated with 300 nm of thermal oxide (SiO2) were purchased from Silicon Valley Microelectronics.
Sahu A., Russ B., Liu M., Yang F., Zaia E.W., Gordon M.P., Forster J.D., Zhang Y.Q., Scott M.C., Persson K.A., Coates N.E., Segalman R.A, & Urban J.J. (2020). In-situ resonant band engineering of solution-processed semiconductors generates high performance n-type thermoelectric nano-inks. Nature Communications, 11, 2069.
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