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For immunohistochemistry, mice were perfused intracardially with 4% paraformaldehyde phosphate buffer. Serial coronal sections were prepared and blocked by PBS containing 3% BSA and 0.3% Triton‐X100 and then incubated with primary antibodies overnight at room temperature as the following: rabbit anti‐Axin2 (Abcam, ab109307, 1:200), rat anti‐MBP (Merck Millipore, MAB386, 1:500), mouse anti‐MT1 receptor (Santa cruz, sc‐390328, 1:50), mouse anti‐NeuN (Abcam, ab104224, 1:600), rabbit anti‐NG2, (Abcam, ab255811, 1:150), rabbit anti‐GFAP (Abcam, ab207165, 1:500), mouse anti‐Neurofliment (Abcam, ab7795, 1:200), rabbit anti‐Olig2(Abcam, ab109186, 1:200), mouse anti‐CC1(Sigma‐Aldrich, OP80, 1:100), mouse anti‐Flag (Sigma‐Aldrich, F1804, 1:100), Rabbit anti‐β‐catenin (Abcam, ab16051, 1:200), Rabbit anti‐vglut1 (Synaptic Systems Cat. No.135308, 1:200). After washing with PBS, corresponding secondary antibodies conjugated with Alexa Fluro 488 (donkey anti‐rabbit, donkey anti‐mouse, Jackson ImmunoResearch, 1:500, AB_2313584, AB_2338845) or Alexa Fluro 594 (donkey anti‐rat, donkey anti‐mouse, donkey anti‐rabbit, Jackson ImmunoResearch, Jackson 1:500, RRID:AB_2338372, AB_2338871, AB_2338059) were incubated with the sections for 2–4 h at room temperature protected from light. After washing with PBS, sections were counterstained with Hoechst33324 (1:1000, Sigma) for 20 min.