PBMC or NK cells were cultured overnight before treating with purified IFN‐α (Sigma) or recombinant IFN‐α (Miltenyi Biotec), recombinant IL‐12 (R&D systems and Peprotech) or recombinant IL‐15 (Miltenyi Biotec) as described in the text and figure legends. For IFN‐I neutralization, a cocktail of anti‐human interferon α/β receptor chain 2 antibody (clone MMHAR‐2), anti‐human interferon‐α (sheep polyclonal) and anti‐human interferon‐β (sheep polyclonal; all from PBL Assay Science) or a control cocktail of mouse IgG2a (BioLegend) and sheep serum (Sigma) was used, as described previously [25 (link)].
Recombinant ifn α
Manufactured by Miltenyi Biotec
Sourced in United Kingdom
Recombinant IFN-α is a cytokine produced through recombinant DNA technology. It is a type I interferon that plays a role in immune system regulation.
Automatically generated - may contain errors
2 protocols using recombinant ifn α
1
Cytokine Stimulation of Immune Cells
2
FTY720 treatment regulates secondary RSV infection
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Six doses of 25μg of FTY720 (Enzo Life Sciences, UK) in 250μl were injected i.p. at days -2, -1, 0, 1, 2, 3 relative to secondary RSV infection. At day 4, animals were culled 10 min after intravenous injection with 2 μg (in 200 μl) of anti-CD45 BUV395 (30-F11, BD). To check staining levels blood samples were withdrawn postmortem from the femoral vein, cells were isolated and assessed by flow cytometry.
At 6h and 18h after primary infection mice were treated i.n. after light anesthesia with 500 ng of recombinant IFN-α (Miltenyi Biotech, UK) in 100μl.
At 6h and 18h after primary infection mice were treated i.n. after light anesthesia with 500 ng of recombinant IFN-α (Miltenyi Biotech, UK) in 100μl.
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