Madin–Daby canine kidney cells (MDCK) and SP2/0 mouse myeloma cells were provided by the Key Laboratory of Animal Immunology, Henan Academy of Agricultural Science. RPMI Medium 1640 and Dulbecco’s modified Eagle’s medium (DMEM) were obtained from Solarbio Life Sciences, China. Fetal bovine serum (FBS) was obtained from Moregate Biotech. Horseradish peroxidase (HRP)-conjugated goat anti-mice secondary antibodies were purchased from Jackson Immuno Research Laboratories, West Grove, PA, USA. All other chemicals used were of reagent grade. Recombinant HA protein expressed in the rice endosperm was provided by the International Associated Research Center of National Animal Immunology.
Dulbecco s modified eagle s medium dmem
Manufactured by Solarbio
Sourced in China, United States
Dulbecco's modified Eagle's medium (DMEM) is a widely used cell culture medium. It provides a balanced salt solution and essential nutrients to support the growth and maintenance of a variety of cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions)
Trypsin, by Solarbio (5 mentions)
Fetal bovine serum (fbs), by Solarbio (4 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Solarbio (2 mentions)
Fetal bovine serum (fbs), by ExCell Bio (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Solarbio (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Solarbio (2 mentions)
Foetal bovine serum fbs, by Solarbio (1 mentions)
25 cm2 cell culture flask, by Corning (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Ir780 iodide, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Hoechst, by Beyotime (1 mentions)
Prestoblue, by Thermo Fisher Scientific (1 mentions)
Poloxamer 407, by BASF (1 mentions)
Phospho sapk jnk, by Cell Signaling Technology (1 mentions)
34 protocols using dulbecco s modified eagle s medium dmem
1
MDCK and SP2/0 Cell Culture Protocol
2
In vitro Larval Invasion Assay
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Normal mouse intestinal epithelial cells (IECs) were previously isolated from BALB/c mouse intestines and maintained for the in vitro larval invasion assay [29 (link)]. Investigations revealed that the IECs were susceptible to larval invasion of T. spiralis, in contrast to C2C12 cells from mouse striated muscle myoblasts, which were resistant to larval invasion. IECs were used in the in vitro larval invasion assay, and C2C12 cells were used as a negative control. IECs and C2C12 cells were cultured in 25 cm2 cell culture flasks (Corning, NY, USA) in Dulbecco’s modified Eagle’s medium (DMEM) (Solarbio, Beijing, China) supplemented with 5% foetal bovine serum (FBS) (Solarbio, China) and incubated in 5% CO2 at 37 °C. To maintain the cell cultures, the medium was renewed every 2 or 3 days, and cell monolayers were digested with 0.25% trypsin (Solarbio, China). Preparation of proteins from IEC and C2C12 lysates was performed as described above [31 (link)].
3
Ethanol-Induced Hepatic and Myeloid Cell Responses
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HepG2 and AML12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Solarbio company, Beijing, China) (containing 10% fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, and 1‰ amphotericin B) and was maintained at 37°C, 95% air humidity, and 5% CO2, taken from Cell Bank of Typical Culture Preservation Committee of Chinese Academy of Sciences. The cells were treated with 100 mM ethanol or control for 24 h together with medicated serum of QGHXR, QGR, and HXR (100 mg/L). The description of specific ALI cell model methods and dose selection of medicated serum is shown in Supplementary Material . Then, the cells were harvested and collected for RNA and total protein extraction.
4
Culturing Human Liver Cell Lines
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The human normal liver cell line (HL-7702) and 3 HCC cell lines (Hep3B, HCCLM3 and Huh-7) were selected to conduct the assays, and they were purchased from the Shanghai Institute of Cell Biology (Shanghai, China). These cell lines were cultured in high-glucose Dulbeccos modified Eagles medium (DMEM; Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) supplemented with 10% fetal bovine serum (FBS; Biological Industries, Kibbutz Beit-Haemek, Israel), 100 µg/ml streptomycin and 100 U/ml penicillin at 37°C, in a 5% CO2 and 95% humidity cell culture incubator.
5
In Vitro Cell Viability Assay
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Zein (Meilun Biological, Dalian, China). N2'-deacetyl-N2'-(3-mercapto-1-oxopropyl)-maytansine (DM1 > 98%, Bright Gene Co., Ltd., Suzhou, China). Dimethyl sulfoxide (DMSO, Bailunsi, Tianjin, China). 2-[2-[2-Chloro-3-[(1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene) ethylidene]-1-cyclohexen-1-YL] ethenyl]-3,3-dimethyl-1-propylindolium iodide (IR-780 iodide, Alfa Aesar, Tianjin, China). Hoechst (Beyotime Biotechnology, Shanghai, China). Cell Counting Kit-8 (CCK-8, Dojindo, Shanghai, China). Dulbecco's modified eagle's medium (DMEM, Solarbio, Beijing, China). Fetal bovine serum (FBS, Gibco, Grand Island, NY). Trypsin-EDTA (Gibco). All materials were used without further purification.
6
Formulation and Characterization of IRB-Loaded HP-β-CD/Poloxamer 407 Nanoparticles
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HP-β-CD (C63H112O42) was obtained from Sinopharm (Beijing, People’s Republic of China). Poloxamer 407 (EO106PO70EO106) was generously provided by BASF (Ludwigshafen, Germany). Crude IRB (C25H28N6O, purity more than 99%) was provided by Huahai Pharma Ltd., (Zhejiang, People’s Republic of China) and used as-received. High performance liquid chromatography (HPLC)-grade acetonitrile (C2H3N) and methanol (CH4O) were purchased from Concord (Tianjin, People’s Republic of China). PrestoBlue® was purchased from Life Technologies (Carlsbad, CA, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from Solarbio (Beijing, People’s Republic of China). Purified water was used throughout this study. All the other reagents and chemicals used were of analytical grade or better.
7
Exploring anti-inflammatory mechanisms in vitro
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Dulbecco’s Modified Eagle’s Medium (DMEM) and neutral red were purchased from Solarbio (Beijing, China). Fetal Bovine Serum (FBS) was purchased from Gibco (Grand Island, NY, USA). Nitric oxide kit was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). TNF-α and IL-6 ELISA kit were obtained from Beijing 4A Biotech Co, Ltd (Beijing, China). Primer iNOS, TNF-α, IL-6, TLR4 and MyD88 were purchased from Thermo Fisher scientific (Shanghai, China). Reactive Oxygen Species Assay Kit were purchased from Beyotime Biotechnology (Shanghai, China). PrimeScriptTMRT reagent kit with gDNA Eraser kit and TB Green TM Ex TaqTM II (Tli RNadeH Plus) and Bulk kit were purchased from TaKaRa. Antibody NF-κB p65, phospho-NF-κB p65, p38 MAPK, phospho-p38 MAPK, SAPK/JNK, phospho-SAPK/JNK, p44/p22 MAPK and phospho-p44/p22 MAPK were purchased from Cell Signaling (Beverly, MA, USA). NF-κB inhibitor (PDTC), ERK inhibitor (PD98059), JNK inhibitor (SP600125) and p38 inhibitor (SB203580) Lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA)
8
Chitosan Oligosaccharide and Triptolide Interactions
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Triptolide was provided by Xi’an Haoxuan Biotechnology Co. Ltd. in Xi’an, China. Chitosan oligosaccharide (CSO, MW: about 3000 Da) was purchased from Aladdin (Beijing, China) and purified using dialysis. Hyclone fetal bovine serum, Trypsin, Dulbecco’s Modified Eagle’s Medium (DMEM), and Phosphate Buffered Saline (PBS 1X, sterile) were all obtained from Beijing Solarbio Science and Technology Co. Ltd. (Beijing, China). Dimethyl sulfoxide, acetonitrile and methanol were MS-grade reagents provided by Tedia. The water used in the experiments was prepared by a Milli-Q ultrapure water instrument (Millipore, Bedford, MA, USA). Hepatocytes (HL7702) and mouse pancreatic cancer cells (Panco2) were purchased from American type cultures (Manassas, VA, USA). Other chemicals were of commercially available analytical grade.
9
Propagation and Titration of VSV and PRV
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PK-15 cells were purchased from the China Veterinary Culture Collection Center and maintained in Dulbecco’s modified Eagle’s medium (DMEM, Solarbio, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco, USA). The cells were cultured at 37 °C in a humidified incubator containing 5% CO2.
Vesicular stomatitis virus (VSV-Indiana, Indiana strain) and pseudorabies virus (PRV-Bartha, Bartha strain) were purchased from the China Veterinary Culture Collection Center and propagated in PK-15 cells, and a 50% tissue culture infection dose (TCID50) was determined by titration of virus in PK-15 cells.
Vesicular stomatitis virus (VSV-Indiana, Indiana strain) and pseudorabies virus (PRV-Bartha, Bartha strain) were purchased from the China Veterinary Culture Collection Center and propagated in PK-15 cells, and a 50% tissue culture infection dose (TCID50) was determined by titration of virus in PK-15 cells.
10
Cell Culture and Compound Preparation Protocol
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Dulbecco’s
modified Eagle’s
medium (DMEM), trypsin with/without ethylenediaminetetraacetic acid
(EDTA), penicillin–streptomycin, and sterile phosphate-buffered
saline (PBS) were obtained from Solarbio Co., Ltd. (Beijing, China).
Reduced GSH, quercetin, rutin, andrographolide, resveratrol, polydatin,
curcumin, and ursolic acid were obtained from Solarbio Co., Ltd. (Beijing,
China), and all of the plant components are standards with purity
greater than 99%. Fetal bovine serum (FBS) was purchased from Gibico
(Gaithersburg, MD). quercetin stock (25 mM) was dissolved in dimethyl
sulfoxide (DMSO) and preserved under −20 °C. Dextran sulfate
sodium salt (DSS, 36 000–50 000 Da) was purchased
from MP Biomedicals.
modified Eagle’s
medium (DMEM), trypsin with/without ethylenediaminetetraacetic acid
(EDTA), penicillin–streptomycin, and sterile phosphate-buffered
saline (PBS) were obtained from Solarbio Co., Ltd. (Beijing, China).
Reduced GSH, quercetin, rutin, andrographolide, resveratrol, polydatin,
curcumin, and ursolic acid were obtained from Solarbio Co., Ltd. (Beijing,
China), and all of the plant components are standards with purity
greater than 99%. Fetal bovine serum (FBS) was purchased from Gibico
(Gaithersburg, MD). quercetin stock (25 mM) was dissolved in dimethyl
sulfoxide (DMSO) and preserved under −20 °C. Dextran sulfate
sodium salt (DSS, 36 000–50 000 Da) was purchased
from MP Biomedicals.
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