ADSM were pre-cultured for 16 h in DMEM (Wako, High glucose) without FBS before CM was added directly to a final concentration of 50% and the cells were cultured for another 24 h. The glucose and glutamine concentrations in the medium were measured using a Glucose Assay Kit-WST (DOJINDO, Japan) and Glutamine Assay Kit-WST (DOJINDO), respectively. Absorbance at 450 nm was measured using a FluxStation3 (Molecular Devices) according to the manufacturer’s instructions.
Fluxstation3
Manufactured by Molecular Devices
Sourced in United States
The FluxStation3 is a high-performance microplate reader designed for accurate and reliable fluorescence measurements. It features a sensitive detection system and advanced optics to provide precise quantification of fluorescent signals in a wide range of assays.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using fluxstation3
1
ADSM Conditioned Medium Metabolite Analysis
2
Quantification of Tnf Protein in ADSM
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ADSM were cultured in CM for 24 h. The concentration of Tnf protein in the medium was measured using a Mouse Tnf ELISA kit (BioLegend). Absorbance at 450 nm was measured using a FluxStation3 (Molecular Devices) according to the manufacturer’s instructions.
3
Quantifying Serum Ccl20 Levels in Mice
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Ccl20 protein concentration in mice serum was measured using a Mouse Ccl20 ELISA kit (R&D Systems). Absorbance at 450 nm was measured using a FluxStation3 (Molecular Devices) according to the manufacturer’s instructions.
4
Serum CCL20 Quantification in Mice
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Ccl20 protein concentration in mice serum was measured using Mouse Ccl20 ELISA kit (R&D Systems). Absorbance at 450 nm was measured using a FluxStation3 (Molecular Devices, USA) according to the manufacturer's instructions.
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