The study was approved by the Animal Ethics Committee of Wannan Medical College. Four-week-old female thymus-free BALB/c nude mice were randomly divided into the following four groups with three mice in each group to examine tumorigenicity: (i) agomir control group, (ii) GAS5 overexpression + agomir control group, (iii) miR-18a-5p agomir group, and (iv) GAS5 overexpression + miR-18a-5p agomir group. First, T24 cells (stably overexpressing GAS5 or empty vector) were transfected with miR-18a-5p agomir (GenePharma, China) or control agomir (GenePharma) for 48 h. After treatment, the cells were collected and resuspended in PBS, and a single-cell suspension of 2 × 106 cells was inoculated subcutaneously into the right axilla of mice. In addition, to ensure the expression of miR-18a-5p in tumors, we also performed experiments in which agomir-18a-5p NC/agomir-18a-5p was injected directly into implanted tumors at a dose of 10 nmol per mouse (in 20 μL of PBS) twice. The width (W) and length (L) of the tumors were measured every 7 days. Tumor volume was expressed as L × W2/2. The mice were killed after 4 weeks. All tumors were debrided, weighed, and fixed.
Agomir control
Manufactured by GenePharma
Sourced in China
Agomir control is a synthetic oligonucleotide designed to serve as a control for agomir experiments. It is a crucial tool for validating the specificity and efficacy of agomir-based gene regulation studies.
Automatically generated - may contain errors
Lab products found in correlation
Antagomir control, by GenePharma (2 mentions)
Mir 18a 5p agomir, by GenePharma (1 mentions)
Mimic control, by GenePharma (1 mentions)
Melatonin, by Merck Group (1 mentions)
Stattic, by Selleck Chemicals (1 mentions)
Pten sirna, by GenePharma (1 mentions)
Mir 21 5p mimic, by GenePharma (1 mentions)
Recombinant human tgf β1, by R&D Systems (1 mentions)
Agomir, by GenePharma (1 mentions)
Bylipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Scramble control, by RiboBio (1 mentions)
Plenti, by OriGene (1 mentions)
Mirzip control, by System Biosciences (1 mentions)
Agomir 9, by GenePharma (1 mentions)
Antagomir 9, by GenePharma (1 mentions)
Agomir 9, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
5 protocols using agomir control
1
Modulating Tumor Growth with GAS5 and miR-18a-5p
2
Melatonin and TGF-β1 Signaling Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melatonin was acquired from Sigma (Cat. No M5250, SIGMA‐ALDRICH). Recombinant human TGF‐β1 was purchased from R&D Systems. Stattic was purchased from Selleckchem (Cat. No S7024, Selleckchem). Control mimics, miR‐21‐5p mimics, negative control siRNAs, Spry1 siRNA and PTEN siRNA were designed and synthesized by Shanghai GenePharma. Control agomir and mmu‐miR‐21‐5p agomir were also purchased from Shanghai GenePharma.
3
Modulating miR-142-5p Expression in SH-SY5Y Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MiR-142-5p antagomir, antagomir control, agomir, and agomir control were obtained
from Shanghai GenePharma Co, Ltd (Shanghai, China). SH-SY5Y cell lines were
seeded into a 6-well plate at a density of 1 × 106 cells/mL and then were
transfected with 50 nM miR-142-5p agomir/antagomir or their controls by
Lipofectamine 2000 (Invitrogen) according to the manufacturer’s guidance. After
transfection of 72 hours, the efficiency of transfection was used quantitative
real-time polymerase chain reaction (qRT-PCR) to measure.
from Shanghai GenePharma Co, Ltd (Shanghai, China). SH-SY5Y cell lines were
seeded into a 6-well plate at a density of 1 × 106 cells/mL and then were
transfected with 50 nM miR-142-5p agomir/antagomir or their controls by
Lipofectamine 2000 (Invitrogen) according to the manufacturer’s guidance. After
transfection of 72 hours, the efficiency of transfection was used quantitative
real-time polymerase chain reaction (qRT-PCR) to measure.
4
Investigating miR-210 Regulation In Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The miR-210 antagomir and scramble control were purchased from RiboBio Co., Guangzhou, China. The miR-210 agomir and agomir control were synthesized by GenePharma, Shanghai, China. The miR-Zip Control and miR-Zip-210 plasmids were purchased from System Bioscience, Palo Alto, CA, USA. The pLenti and pLenti-STMN1- Myc-DDKvectors were obtained from OriGene, Rockville, MD, USA. The pCMVΔR8.9, pDVsVg, and pLKO.1-shLuc vectors and the shRNA against STMN1 were obtained from the National RNAi Core Facility of Taiwan for gene silencing. All clones were verified by direct sequencing.
5
Modulating Hes1 Expression by miRNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The agomiR control, agomiR-9, antagomiR control, antagomiR-9, siRNA scrambled control, and Hes1 siRNA were purchased from GenePharma (GenePharma). Transfection of 20 nM agomiR-9/antagomiR-9/Hes1 siRNA or their corresponding controls was performed using the Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s instruction.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!