Blood samples were drawn on admission. The median (interquartile range, IQR) time from symptom onset to blood sampling was 17(8–28) hours. Plasma N-terminal fragment of B-type natriuretic peptide (Nt-proBNP) was measured using homogeneous chemiluminescence immunoassay on a Vista analyzer (SIEMENS). Blood glucose was assessed by the enzymatic method on a Vitros 950 analyzer (Ortho Clinical Diagnostics, Rochester, NY). Plasma creatinine levels were measured on a Vitros 950 analyzer (Ortho Clinical Diagnostics, Rochester, NY). Glomerular Filtration Rate was calculated by CKD-EPI formula [21 ]. C-reactive protein (CRP) was determined on a dimension Xpand (Dade Behring, Deerfield, IL) using enzymatic methods. The CRP level was dichotomized at 3 mg/L for more clinical relevance.
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