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Uas bsk rnai

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The UAS-Bsk-RNAi is a genetic tool used to study gene function in Drosophila. It contains an RNA interference (RNAi) construct that can be used to specifically knockdown the expression of the basket (bsk) gene, which encodes the Drosophila homolog of the c-Jun N-terminal kinase (JNK). The UAS-Bsk-RNAi construct can be expressed in a spatially and temporally controlled manner using the Gal4/UAS system.

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Lab products found in correlation

Uas mcherry rnai, by Bloomington Drosophila Stock Center (2 mentions) Uas hep rnai, by Bloomington Drosophila Stock Center (2 mentions) Ok6 gal4, by Bloomington Drosophila Stock Center (1 mentions) W1118, by Bloomington Drosophila Stock Center (1 mentions) Ppk gal4, by Bloomington Drosophila Stock Center (1 mentions) Elav gal4c155, by Bloomington Drosophila Stock Center (1 mentions) Uas ecr rnai, by Bloomington Drosophila Stock Center (1 mentions) Uas p35, by Bloomington Drosophila Stock Center (1 mentions) Uas scrib rnai, by Bloomington Drosophila Stock Center (1 mentions) Uas apkc rnai, by Bloomington Drosophila Stock Center (1 mentions) Uas mkk4 rnai, by Bloomington Drosophila Stock Center (1 mentions) Uas p38b rnai, by Bloomington Drosophila Stock Center (1 mentions) Uas p38a rnai, by Bloomington Drosophila Stock Center (1 mentions)

4 protocols using uas bsk rnai

1

Drosophila Genetic Strains for Metabolism Research

Cited 3 times
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Animals were reared at room temperature on standard food containing cornmeal, molasses, and yeast in all experiments except for those involving high-protein, intermediate-fat diet (HPIFD) and high-fat diet (HFD). The detailed composition of these diets is listed in Supplementary Table 1. The following Drosophila strains were used in the study: (1) y w, (2) foxoΔ94/TM6B, Tb1, (3) Tl/TM3, Sb1, (4) RelE38, (5) UAS-foxoRNAi, (6) UAS-TlRNAi, (7) y1sc*v1sev21;UAS-RelRNAi, (8) Myo1A-GAL4 (enterocytes), (9) r4-GAL4 (fat body), (10) c135-GAL4 (path-GAL4), (11) UAS-dipl2, (12) UAS-InRWT, (13) UAS-InRA1325D (constitutively activated UAS-InRCA), (14) UAS-AktWT, (15) UAS-AktΔPH (constitutively activated UAS-AktCA), (16) UAS-mCherrynls, (17) pros-GAL4 (enteroendocrine cells), (18) esg-GAL4 (adult midgut precursors), (19) elav-GAL4 (neurons), (20) C147-GAL4 (salivary glands) and (21) UAS-bskRNAi (Bloomington Drosophila Stock Center); (22) Mef2-GAL4 (mesoderm; ref. 63 (link)); (23) Pnglex14, (24) UAS-PnglWT and (25) UAS-PnglC303A (ref. 25 (link)); (26) PBac{Pnglwt}VK31 (Pngl duplication; ref. 27 (link)); (27) Dp(1;3)DC102, PBac{DC102}VK33 (AMPKα duplication; ref. 64 (link)); and (28) UAS-dipl6 (ref. 65 (link)).
Pandey A., Galeone A., Han S.Y., Story B.A., Consonni G., Mueller W.F., Steinmetz L.M., Vaccari T, & Jafar-Nejad H. (2023). Gut barrier defects, intestinal immune hyperactivation and enhanced lipid catabolism drive lethality in NGLY1-deficient Drosophila. Nature Communications, 14, 5667.
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2

Drosophila Genetics Toolbox Utilization

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The following stocks were obtained from Tsinghua RNAi Stock Center: UAS-babo-RNAi (THU5256), UAS- uba1-RNAi (THU2127).
The following stocks were obtained from Bloomington Stock Center (BSC): w1118, ok6-gal4, elav-gal4 (c155), PPK-Gal4, mCD8-GFP, UAS-EcR-RNAi (BSC9327), UAS-EcR-B1DN (BSC9452), UAS-bsk-RNAi (BSC36643), UAS-mCherry-RNAi (BSC35785).
UAS-tubulin-GFP (Dr. Xing Liang, University of Tsinghua).
Xu W., Kong W., Gao Z., Huang E., Xie W., Wang S, & Rui M. (2023). Establishment of a novel axon pruning model of Drosophila motor neuron. Biology Open, 12(1), bio059535.
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3

Genetic Screening in Drosophila Development

Cited 1 time
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All stocks were grown under standard conditions. Stocks used: apterousME-NLS::dsRed[60] (link), Df(3L)sydA2 and sydZ4[45] (link), Khc8[61] (link), Dhc64C4−19[62] (link), Klc8ex94[63] (link), Dlc90F05089[64] (link), lis1K11702[65] (link), lis1G10.14[66] (link), raps193[67] (link), twist-Gal4[68] (link), Dmef2-Gal4[69] (link), Stripe-Gal4 (gift from T. Volk), Elav-Gal4 (gift from E. Lai), UAS-GFP-JIP3 (this study), bsk1 and bsk2[49] (link), UAS-Bsk-DN and UAS-Hep-Act[50] (link). Bloomington Drosophila Stock Center: clip190KG06490 (14493), UAS-Tak1-RNAi (33404, 35180), UAS-Hep-RNAi (28710, 35210), UAS-Bsk-RNAi (31323, 32977, 35594, 36643), UAS-mCherry-RNAi (35785). Vienna Drosophila RNAi Center: UAS-Syd-RNAi KK109225 (v101459), UAS-Syd-RNAi GD12383 (v35346). Mutants were balanced and identified using CTG (CyO, Twi-Gal4, UAS-2xeGFP) and TTG (TM3, Twi-Gal4, UAS-2xeGFP) [70] (link).
Schulman V.K., Folker E.S., Rosen J.N, & Baylies M.K. (2014). Syd/JIP3 and JNK Signaling Are Required for Myonuclear Positioning and Muscle Function. PLoS Genetics, 10(12), e1004880.
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4

Drosophila Genetic Manipulation Protocols

Cited 2 times
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All crosses and staging were performed at 25°C unless otherwise noted. w1118 was used as wild-type. Stocks are described in FlyBase (http://flybase.org/). pucE69, UAS-P35 (B#5073), UAS-Scrib-RNAi (B#29552), UAS-Bsk-RNAi (B#32977), UAS-Moesin-myc (B#8631), UAS-Moesin-RNAi (B#8629), UAS-Mekk1-RNAi (B#28587), UAS-Ask1-RNAi (B#32464), UAS-Hep-RNAi (B#28710), UAS-Mkk4-RNAi (B#35140), UAS-p38b-RNAi (B#35252), UAS-p38a-RNAi (B#34744), bsk1 (B#3088), UAS-Baz-RNAi (B#39072), UAS-aPKC-RNAi (B#25946) and “Dlg-RNAi #2” (B#35286) were provided by the Bloomington Drosophila Stock Center (BDSC); Zip1 by T. Wolff (NIH Janelia Research Campus); patched-GAL4 by R. Cagan (Mount Sinai School of Medicine, New York); UAS-Cdc42- RNAi and UAS-Rho1-RNAi were described previously [11 (link),12 (link)]; “UAS-Dlg-RNAi #1”(V#41134), UAS-Rok-RNAi (V#3793), and UAS-Wnd-RNAi (V#103410) were provided by the Vienna Drosophila RNAi Center (VDRC); UAS-Slpr-RNAi, UAS-Wengen-RNAi, and UAS-Tak1-RNAi were provided by R. Fehon (University of Chicago); UAS-Egr was provided by M. Vidal (Beatson Institute, Glasgow, UK). MARCM clones were generated by heat shock at 37C for 1h and dissected 40 to 48 hours after clone induction (ACI).
Schimizzi G.V., Maher M.T., Loza A.J, & Longmore G.D. (2016). Disruption of the Cdc42/Par6/aPKC or Dlg/Scrib/Lgl Polarity Complex Promotes Epithelial Proliferation via Overlapping Mechanisms. PLoS ONE, 11(7), e0159881.
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