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Griess assay

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The Griess Assay is a colorimetric method used to detect and quantify nitrite (NO2-) in biological samples. It involves the reaction of nitrite with sulfanilamide and N-(1-naphthyl)ethylenediamine dihydrochloride (NED), resulting in the formation of a purple azo dye that can be measured spectrophotometrically.

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Lab products found in correlation

Enzyme linked immunosorbent assay, by BD (1 mentions) Amplex ultrared, by Thermo Fisher Scientific (1 mentions) Ph meter, by Mettler Toledo (1 mentions)

Spelling variants of this product

Griess reaction colorimetric assay (3 citations) Griess reaction assay (3 citations) Griess nitrite/nitrate colorimetric assay (2 citations) Griess assay kit (2 citations) Griess reaction colorimetric assay kit (2 citations)

5 protocols using griess assay

1

Nitrate/Nitrite Quantification by Griess Assay

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Nitrate/nitrite content in the media was measured with the Griess Assay (Cayman, Ann Arbor, MI) via the manufacturer's specifications. Briefly, this assay converts nitrate to nitrite with the enzyme nitrate reductase then uses the Griess Reagents to convert nitrite into the Azo chomophore compound that which can be evaluated through reading the absorbance at 540 nm. Media from the four conditions were thawed on ice and spun at 1000× g to remove debris. The supernatant was assayed in triplicate. Interassay coefficient of variation (CV) was 6.98 ± 0.74% and intra‐assay CV was 8.07 ± 2.69%.
Witkowski S., Guhanarayan G, & Burgess R. (2016). Glucose and acute exercise influence factors secreted by circulating angiogenic cells in vitro. Physiological Reports, 4(3), e12649.
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2

Quantifying Nitric Oxide Production

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Nitric oxide and its metabolites were measured in BM-MDRC cell culture supernatants by Griess Assay (Cayman Chemicals, Ann Arbor, MI) which uses nitrate reductase and converts nitrate to nitrite and measures total NO production 32 (link). Quantitation of lung cells with potential to produce NO were determined by first staining collagenase extracted lung cells with the fluorescent indicator for NO, DAF-FM-DA followed by flow cytometry as described before 32 (link).
Wang Y., Jin T.H., Farhana A., Freeman J., Estell K., Zmijewski J., Gaggar A., Thannickal V.J., Schwiebert L.M., Steyn A.J, & Deshane J.S. (2014). Exposure to cigarette smoke impacts myeloid-derived regulatory cell function and exacerbates airway hyper-responsiveness. Laboratory investigation; a journal of technical methods and pathology, 94(12), 1312-1325.
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3

Quantifying Nitric Oxide Production

Cited 1 time
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Nitric oxide and its metabolites were measured in BM-MDRC cell culture supernatants by Griess Assay (Cayman Chemicals, Ann Arbor, MI) which uses nitrate reductase and converts nitrate to nitrite and measures total NO production 32 (link). Quantitation of lung cells with potential to produce NO were determined by first staining collagenase extracted lung cells with the fluorescent indicator for NO, DAF-FM-DA followed by flow cytometry as described before 32 (link).
Wang Y., Jin T.H., Farhana A., Freeman J., Estell K., Zmijewski J., Gaggar A., Thannickal V.J., Schwiebert L.M., Steyn A.J, & Deshane J.S. (2014). Exposure to cigarette smoke impacts myeloid-derived regulatory cell function and exacerbates airway hyper-responsiveness. Laboratory investigation; a journal of technical methods and pathology, 94(12), 1312-1325.
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4

Inflammatory Markers in Sputum and Serum

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All the inflammatory parameters from sputum (IL-8, TNF-α) and serum (C-reactive protein [CRP]) were measured in triplicate using commercially available enzyme-linked immunosorbent assays (BD, Franklin Lakes, NJ, USA) according to the manufacturer’s instructions. Nitrogen oxide (nitrite and nitrate) levels in sputum were detected by measuring nitrate and nitrite content using the Griess assay (Cayman Chemical, Ann Arbor, MI, USA) as recommended by the manufacturer.
Unfried K., Krämer U., Sydlik U., Autengruber A., Bilstein A., Stolz S., Marini A., Schikowski T., Keymel S, & Krutmann J. (2016). Reduction of neutrophilic lung inflammation by inhalation of the compatible solute ectoine: a randomized trial with elderly individuals. International Journal of Chronic Obstructive Pulmonary Disease, 11, 2573-2583.
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5

Quantification of Reactive Species in Plasma-Irradiated Cell Culture

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For the detection of reactive species introduced into the cell culture medium following gas plasma irradiation, several different assays were applied. Hydrogen peroxide (H2O2) was quantified utilizing the Amplex Ultra Red assay kit (ThermoFisher) according to the manufacturers’ instructions. For the detection of nitrite (NO2−) inside the medium, a deterioration product of nitric oxide (NO), the Griess assay (Cayman Chemical) was carried out. Hypochlorous acid (HOCl) was detected by the colorimetric taurine chloramine assay, as previously described.31 (link) The measurements were performed using a multiplate reader (Tecan Infinite F200 PRO) at λex/em = 535 nm/590 nm for fluorescence detection (H2O2), or the (Tecan Infinite M200 PRO) at λ = 540 nm (NO2−) and at λ = 645 nm (HOCl) for absorbance detection. The pH levels of gas plasma-irradiated cell culture medium were quantified using a pH-meter (Mettler Toledo).
Mahdikia H., Saadati F., Freund E., Gaipl U.S., Majidzadeh-a K., Shokri B, & Bekeschus S. (2020). Gas plasma irradiation of breast cancers promotes immunogenicity, tumor reduction, and an abscopal effect in vivo. Oncoimmunology, 10(1), 1859731.
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