Hcc1806

HCT 116, MDA-MB-231 and HCC1806 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). EW8, Rh41 and HCC1806 cells were maintained in RPMI 1640 (Lonza, Portsmouth, NH, USA) supplemented with 10% fetal bovine serum (FBS; Life Technologies), TC-71 was maintained in IMDM (Lonza) supplemented with 10% FBS and ITS (Life Technologies), HCT 116 cells were maintained in McCoy's 5A (ATCC) supplemented with 10% FBS, and MDA-MB-231 cells were maintained in DMEM (Lonza) supplemented with 10% FBS at 37 °C with 5% CO₂.
Primary MEF cells were derived from E13.5 or E14.5 embryos. The p53^−/− and ARF^−/− MEF cells are generous gifts from Dr. Martine F. Roussel (St. Jude Children's Research Hospital). MEF cells were maintained in DMEM (Lonza) that contained 10% FBS, 1 × MEM non-essential amino acid, 55 μM 2-mercaptoethanol and 10 μg/ml gentamicin (Life Technologies) at 37 °C with 5% CO₂. Early passage (P2-P5) MEF cells were used for the experiments. Exceptions were p53^−/− and ARF^−/− MEF cells.

Breast carcinoma cell lines (MCF7, T47D, HCC1806, HCC1937, BT549, MDA-MB-453, SUM149, MDA-MB-468, SKBR3, BT20, and BT549) were kindly provided by Dr. M. Götte (Department of Gynecology and Obstetrics, Münster, Germany) and Dr. B. Greve (Department of Radiation Oncology, Münster, Germany). Cells were maintained in DMEM/high glucose medium (Lonza, Basel, Switzerland) (SKBr3, MDA-MB-453, and MDA-MB-468) or in RPMI medium (MCF7, T47D, HCC1806, HCC1937, BT20, and BT549). Both media were supplemented with 10% fetal calf serum (FCS; Gibco, Waltham, MA, USA) and 100 U/mL penicillin–streptomycin (PS; Gibco). SUM149 cells were cultured in Dulbecco’s modified Eagle’s medium-F12 (1:1) with 5% FCS, insulin (5 μg/mL) (Merk, Darmstadt, Germany), and hydrocortisone (1 μg/mL; Qiagen, Hilden, Germany). MDA-MB-231 cell line was obtained from DSMZ (Leibniz Institute DSMZ–German Collection of Microorganisms and Cell Cultures) and was grown in DMEM/high glucose medium containing 10% FCS and 100 U/mL PS. HUVECs were kindly provided by Dr. D. Vestweber (Max Planck Institute of Molecular Biomedicine, Münster, Germany) or purchased from Promocell (Heidelberg, Germany) and cultured up to passage five in ECGM-2 medium supplemented with SupplementPack (PromoCell).