Hmsc bm

Manufactured by PromoCell

Sourced in Germany

The HMSC-BM is a piece of lab equipment designed for the isolation and cultivation of human mesenchymal stem cells from bone marrow. It provides a controlled environment for the expansion of these cell types.

Automatically generated - may contain errors

Lab products found in correlation

C 28010, by PromoCell (1 mentions) Hdlec, by PromoCell (1 mentions) Ecgm mv, by PromoCell (1 mentions) Mscgm, by PromoCell (1 mentions) C 22020, by PromoCell (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Sodium pyruvate, by Merck Group (1 mentions) Mesenchymal stem cell growth medium, by PromoCell (1 mentions) Mesenchymal stem cell growth medium 2, by PromoCell (1 mentions) Fungizone, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions) L glutamine and phenol red, by Fujifilm (1 mentions) Hyclone fetal bovine serum fbs, by Cytiva (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

BM-hMSCs HMSCs

5 protocols using hmsc bm

Human Dermal and Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human dermal LEC derived from juvenile foreskin (HDLEC) were purchased from PromoCell GmbH (Heidelberg, Germany) and cultured in endothelial cell growth medium MV (ECGM MV, PromoCell) with the corresponding supplement mix (see C‐22020 PromoCell). LEC in passages 6 and 7 were used for all experiments.
Human MSC derived from bone marrow (hMSC‐BM) were purchased from PromoCell and cultured in MSC growth medium (MSC medium, MSC‐GM, PromoCell) with the corresponding supplement mix (see C‐28010 PromoCell). MSC in passages 6 and 7 were used for all experiments.
Culture medium was changed 3 times a week, and the cells were passaged 1:3 after reaching a confluence of 80%. All cells were cultured at 37°C in an atmosphere of 5% CO₂.

Robering J.W., Weigand A., Pfuhlmann R., Horch R.E., Beier J.P, & Boos A.M. (2018). Mesenchymal stem cells promote lymphangiogenic properties of lymphatic endothelial cells. Journal of Cellular and Molecular Medicine, 22(8), 3740-3750.

+ Open protocol

+ Expand

Culturing Human Bone Marrow-Derived Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human mesenchymal stem cells from bone marrow (hMSC-BM) were purchased from Promocell GmbH (Heidelberg, Germany) and cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, St Louis, MO, USA) supplemented with 10% foetal bovine serum (FBS), 2% antibiotic mixture (penicillin–streptavidin, glutamine, fungizine), 1% non-essential amino acids and 1% sodium pyruvate (Sigma-Aldrich) at 37°C, 5% CO₂. MSCs were used for experiments between passages 1 and 4.

Lewis N.S., Lewis E.E., Mullin M., Wheadon H., Dalby M.J, & Berry C.C. (2017). Magnetically levitated mesenchymal stem cell spheroids cultured with a collagen gel maintain phenotype and quiescence. Journal of Tissue Engineering, 8, 2041731417704428.

+ Open protocol

+ Expand

hMSC-BM Expansion and Proliferation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

hMSC-BM (12974, PromoCell) were cultured in ready-to-use specific media (Mesenchymal Stem Cell Growth medium, C-28009, PromoCell). Cells were expanded and passaged up in an incubator with 5% CO₂ at 37 °C and 95% humidity. Sterilized tissue patches were equilibrated with the MSC media for 24 hrs. prior to cell culture. Cells were harvested and resuspended in media with a density of 1 × 10⁶ cells/mL aseptically. Approximately 30,000 cells were on the top of each tissue. The proliferation/toxicity analysis was performed at each time point (24 h, day 7, day 14 after seeding). The analysis was evaluated by the WST-1 assay, Live/dead staining, and DNA quantification.

Tondato S., Moro A., Butt S., Todesco M., Sandrin D., Borile G., Marchesan M., Fabozzo A., Bagno A., Romanato F., Imran S.J, & Gerosa G. (2023). Tergitol Based Decellularization Protocol Improves the Prerequisites for Pulmonary Xenografts: Characterization and Biocompatibility Assessment. Polymers, 15(4), 819.

+ Open protocol

+ Expand

Genome Editing of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human mesenchymal stem cells from bone marrow (hMSC-BM) derived from healthy donors (PromoCell, Heidelberg, Germany) were expanded with Mesenchymal Stem Cell Growth Medium 2 (PromoCell) containing 1% penicillin/streptomycin. Electroporation of Cas9/gRNA complexes was performed using the same parameters as those used for T cells.

Ito Y., Inoue S., Nakashima T., Zhang H., Li Y., Kasuya H., Matsukawa T., Wu Z., Yoshikawa T., Kataoka M., Ishikawa T, & Kagoya Y. (2023). Epigenetic profiles guide improved CRISPR/Cas9-mediated gene knockout in human T cells. Nucleic Acids Research, 52(1), 141-153.

+ Open protocol

+ Expand

Human Bone Marrow Stromal Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Commercially available human BMSCs were obtained (hMSC-BM; PromoCell, Heidelberg, Germany; C-12974, lot number: 412Z022.4) as described previously.² BMSCs were cultured with a complete culture medium, namely, Dulbecco's modified Eagle's medium (with low glucose levels; 2 mg/mL) containing L-glutamine and phenol red (DMEM; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), supplemented with 20% HyClone fetal bovine serum (FBS; Cytiva, Tokyo, Japan), 1% penicillin/streptomycin, 1·25 mg/mL fungizone (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA), 1% HEPES (Life Technologies, Thermo Fisher Scientific), and 0·1% bFGF (Kaken Pharmaceutical Co., Ltd., Tokyo, Japan) in a humidified atmosphere (20% O_2, 5% CO₂, 37°C) according to the manufacturer's instructions. The medium was replaced twice per week, and BMSCs from the fourth passage process were used (six passages in total similar to that with RECs).

Ukeba D., Yamada K., Suyama T., Lebl D.R., Tsujimoto T., Nonoyama T., Sugino H., Iwasaki N., Watanabe M., Matsuzaki Y, & Sudo H. (2022). Combination of ultra-purified stem cells with an in situ-forming bioresorbable gel enhances intervertebral disc regeneration. EBioMedicine, 76, 103845.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!