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Jnk1 2 sc 571

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JNK1/2 (sc-571) is a lab equipment product manufactured by Santa Cruz Biotechnology. It is a primary antibody that targets the c-Jun N-terminal kinase 1/2 (JNK1/2) protein.

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Lab products found in correlation

Hsp90 h 114, by Santa Cruz Biotechnology (2 mentions) P thr202 tyr204 erk1 2, by Santa Cruz Biotechnology (2 mentions) P mek1 2 ser217 221, by Cell Signaling Technology (2 mentions) P thr183 tyr185 jnk1 2, by Cell Signaling Technology (2 mentions) P ampkα thr172, by Cell Signaling Technology (2 mentions) P ser536 nfκb p65, by Cell Signaling Technology (2 mentions) Erk1 2, by Santa Cruz Biotechnology (2 mentions) P akt ser473, by Cell Signaling Technology (2 mentions) Mek1 2, by Cell Signaling Technology (2 mentions) Ampkα, by Cell Signaling Technology (2 mentions) Phosphatase inhibitor, by LC Laboratories (2 mentions) Chemidoc xrs system, by Bio-Rad (2 mentions)

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JNK1/2 (14 citations)

2 protocols using jnk1 2 sc 571

1

Western Blot Analysis of Signaling Pathways

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Preparation of whole cell lysates and Western blots were performed as previously described54 . Phosphatase Inhibitor (LC laboratories) was added to inhibit phosphatase activity. Antibodies used in this study were: HSP90 (H-114, 1:6,000) and JNK1/2 (sc-571, 1:2,000) from Santa Cruz; p-Thr202/Tyr204 ERK1/2 (4370, 1:2,000), ERK1/2 (9102, 1:2,000), p-Ser217/221 MEK1/2 (9121, 1:1,000), MEK1/2 (4694, 1:1,000), p-Thr183/Tyr185 JNK1/2 (9255S, 1:2,000,), p-Ser473 AKT (9271S, 1:2,000), AKT (9272, 1:2,000), IκBα (9242, 1:2,000), p-Ser536 NFκB p65 (3033, 1:1,000), p-Thr172 AMPKα (2535, 1:2,000) and AMPKα (2532, 1:2,000) from Cell Signaling. Band density was quantitated using the Image Lab software on the ChemiDOC XRS+ system (Bio-Rad). Protein levels were normalized to HSP90 and the phosphorylated forms were normalized to phosphorylation-independent levels of the same protein. Data are presented as mean ± SEM unless otherwise specified. Uncropped blots are shown in Supplementary Fig. 9–11.
Ji Y., Sun S., Shrestha N., Darragh L.B., Shirakawa J., Xing Y., He Y., Carboneau B.A., Kim H., An D., Ma M., Oberhozler J., Soleimanpour S.A., Gannon M., Liu C., Naji A., Kulkarni R.N., Wang Y., Kersten S, & Qi L. (2019). Toll-like receptors TLR2 and TLR4 block the replication of pancreatic β cells in diet-induced obesity. Nature immunology, 20(6), 677-686.
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2

Western Blot Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Preparation of whole cell lysates and Western blots were performed as previously described54 . Phosphatase Inhibitor (LC laboratories) was added to inhibit phosphatase activity. Antibodies used in this study were: HSP90 (H-114, 1:6,000) and JNK1/2 (sc-571, 1:2,000) from Santa Cruz; p-Thr202/Tyr204 ERK1/2 (4370, 1:2,000), ERK1/2 (9102, 1:2,000), p-Ser217/221 MEK1/2 (9121, 1:1,000), MEK1/2 (4694, 1:1,000), p-Thr183/Tyr185 JNK1/2 (9255S, 1:2,000,), p-Ser473 AKT (9271S, 1:2,000), AKT (9272, 1:2,000), IκBα (9242, 1:2,000), p-Ser536 NFκB p65 (3033, 1:1,000), p-Thr172 AMPKα (2535, 1:2,000) and AMPKα (2532, 1:2,000) from Cell Signaling. Band density was quantitated using the Image Lab software on the ChemiDOC XRS+ system (Bio-Rad). Protein levels were normalized to HSP90 and the phosphorylated forms were normalized to phosphorylation-independent levels of the same protein. Data are presented as mean ± SEM unless otherwise specified. Uncropped blots are shown in Supplementary Fig. 9–11.
Ji Y., Sun S., Shrestha N., Darragh L.B., Shirakawa J., Xing Y., He Y., Carboneau B.A., Kim H., An D., Ma M., Oberhozler J., Soleimanpour S.A., Gannon M., Liu C., Naji A., Kulkarni R.N., Wang Y., Kersten S, & Qi L. (2019). Toll-like receptors TLR2 and TLR4 block the replication of pancreatic β cells in diet-induced obesity. Nature immunology, 20(6), 677-686.
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