Twenty-two 8-week-old female Wistar rats (N = 7–8/group; Taconic, Ry, Denmark) were included in this study. The rats were kept in cages with a 12:12-h light–dark cycle, temperature of 21°C ± 2°C and a humidity of 55% ± 5%. Diabetes was induced by an intravenous injection of freshly prepared streptozotocin (55 mg/kg body weight; Sigma-Aldrich, St. Louis, MO) dissolved in 10 mmol/L cold citrate buffer (pH 4.5). Blood glucose was measured in tail capillary blood with a Contour blood glucose meter (Bayer Diabetes Care, Copenhagen, Denmark). Rats were considered diabetic when the blood glucose levels exceeded 15 mmol/L at 48 h after injection of streptozotocin. The rats were divided into a diabetes group and treated group after 1.5 weeks of diabetes. NPH insulin (Eli Lilly, IN) was administered subcutaneous daily for 3 days (1 IU morning and 3 IU afternoon) before the MRI examination. On the day of the MRI examination, the rats received 1 IU in the morning 2–4 h before the MRI scan. The study complied with the guidelines for use and care of laboratory animals and was approved by the Danish Inspectorate of Animal Experiments (License: 2010/561-1938).
Wistar rats
Manufactured by Taconic Biosciences
Sourced in Denmark
Wistar rats are a widely used strain of laboratory rats developed at the Wistar Institute in Philadelphia, Pennsylvania. They are a robust, outbred strain commonly used in various research fields, including pharmacology, toxicology, and behavioral studies. Wistar rats exhibit a calm temperament and are known for their reliable breeding performance and consistent physiological responses.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Merck Group (1 mentions)
Nph insulin, by Eli Lilly (1 mentions)
Sprague dawley rats, by Taconic Biosciences (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Sprague dawley rat, by Charles River Laboratories (1 mentions)
Winnonlin professional, by Pharsight (1 mentions)
Newborn calf serum, by Sartorius (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 with ultraglutamine, by Lonza (1 mentions)
Amicon ultra 4 10 kda centrifugal filter, by Merck Group (1 mentions)
Medium 199, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Gelatin coated culture dishes, by Corning (1 mentions)
Sm7368, by Merck Group (1 mentions)
20 protocols using wistar rats
1
Diabetes Induction and Insulin Treatment in Wistar Rats
2
Rat Brain and Retina Dissection
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We used male Wistar rats (10–12 weeks, 300–370 g, Taconic, Denmark). For the SAH male Sprague-Dawley rats (300–350 g, Taconic, Denmark) were used. The animals were located in an accredited Animal facility at Glostrup Research Institute, Rigshospitalet, Glostrup, Denmark. The animals were provided with standard chow and water ad libitum. The animals were kept at 12 hours light and 12 hours dark cycles. All animal experiments and surgical procedure were performed in accordance with the EU directive 2010/63/EU, Danish Animal Inspectorate (2012-15-2934-389) and ARRIVE guidelines. The study was approved by the Danish Animal Inspectorate. The animals were stunned with CO2 (70%)/O2 (30%) and euthanized by decapitation. The brain and eyes were subsequently removed and placed in a cold +4° C phosphate-buffered saline (PBS) buffer solution. The basilar (BA) and middle cerebral arteries (MCA) from brain vasculature were carefully dissected out under an operating microscope. The retinas were carefully removed from the eyeballs by using surgical tweezers.
3
High-fat Diet Induces Metabolic Disorders in Wistar Rats
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In total, 54 female Wistar rats (body weight 201-237 g; Taconic M and B, Ejby, Denmark) were housed at 21 °C ± 2 °C with a 12-h artificial light cycle. Three animals were housed in each cage with free access to tap water. All animals were allowed to acclimatize on a standard diet (STD) for a week followed by randomization and allocation. Then, half of the rats were fed STD and the other half HFCD ad libitum for 16 wk (Figure 1 ). Diets were obtained from Research Diets (NJ, United States). The STD (D14071501) consisted of the following energy sources: carbohydrates 67 g (70 kcal/100 kcal), fat 4 g (10 kcal/100 kcal), and protein 19 g (20 kcal/100 kcal) per 100 g diet. The HFCD (D14071502) consisted of carbohydrates 19 g (15 kcal/100 kcal), protein 27 g (20 kcal/100 kcal), 1 g cholesterol and fat 39 g (65 kcal/100 kcal) per 100 g diet, including 1% cholesterol and 0.25% cholate.
The study was performed in accordance with local and national guidelines for animal welfare and approved by the Animal Experiments Inspectorate (2014-15-2934-00997).
The study was performed in accordance with local and national guidelines for animal welfare and approved by the Animal Experiments Inspectorate (2014-15-2934-00997).
4
Stress-Induced Anhedonia in Rats
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Adult male Wistar rats (from Taconic, Denmark) aged 5–6 weeks or 100–120 g upon arrival, were individually housed throughout the experiment, at a 12/12-h light/dark cycle, with food and water ad libitum, except when these conditions had to be changed due to the sucrose consumption tests or stress protocol.
Stressed rats were characterized behaviorally with the help of a sucrose consumption test (see below) into two subgroups: depressive-like “anhedonic” and “stress-resilient” animals. For the in vitro electrophysiology we used 47 rats (n = 16 controls, n = 16 anhedonic, n = 15 resilient). For the post mortem histology 18 rats were used (n = 6 controls, n = 6 anhedonic, n = 6 resilient). Twenty rats were investigated in the cognitive test (n = 10 controls, n = 10 anhedonic).
Stressed rats were characterized behaviorally with the help of a sucrose consumption test (see below) into two subgroups: depressive-like “anhedonic” and “stress-resilient” animals. For the in vitro electrophysiology we used 47 rats (n = 16 controls, n = 16 anhedonic, n = 15 resilient). For the post mortem histology 18 rats were used (n = 6 controls, n = 6 anhedonic, n = 6 resilient). Twenty rats were investigated in the cognitive test (n = 10 controls, n = 10 anhedonic).
5
Isolation and Culture of Neonatal Rat Islets
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Neonatal rat islets were isolated from 3- to 6-day-old outbred Wistar rats (Taconic, Ejby, Denmark) as previously described
[11 ]. Islets were precultured and maintained in RPMI 1640 medium (Gibco/Life Technologies) supplemented with 20 mmol/L HEPES buffer, 0.038% (wt/vol) NaHCO3, 100 U/ml penicillin, 100 g/ml streptomycin and 10% (vol/vol) newborn calf serum (NCS) at 37°C in humidified atmospheric air. Prior to experimentation, the islets were randomly distributed to 24-well dishes and incubated for 2 hours in RPMI 1640 medium supplemented as described above, but with 0.5% (vol/vol) NCS relevant reagents were added as indicated.
[11 ]. Islets were precultured and maintained in RPMI 1640 medium (Gibco/Life Technologies) supplemented with 20 mmol/L HEPES buffer, 0.038% (wt/vol) NaHCO3, 100 U/ml penicillin, 100 g/ml streptomycin and 10% (vol/vol) newborn calf serum (NCS) at 37°C in humidified atmospheric air. Prior to experimentation, the islets were randomly distributed to 24-well dishes and incubated for 2 hours in RPMI 1640 medium supplemented as described above, but with 0.5% (vol/vol) NCS relevant reagents were added as indicated.
6
Radioluminography and Pharmacokinetics in Rats
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For the radioluminography experiments, male Wistar rats (Taconic, Ebjy, Denmark) were used which weighed, on average, 200 g on arrival. For the pharmacokinetic experiments, male Sprague Dawley rats (Charles River, Wilmington, MA, USA) with, on average, 200 g body weight on arrival were used.
7
Intravenous Pharmacokinetics of Insulin Analogues in Rats
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Example 5
Intravenous Rat PK
Anaesthetized rats are dosed intravenously (i.v.) with insulin analogues at various doses and plasma concentrations of the test compound is measured using immunoassays or mass spectrometry at specified intervals for 4 hours or more post-dose. Pharmacokinetic parameters are subsequently calculated using WinNonLin Professional (Pharsight Inc., Mountain View, Calif., USA).
Non-fasted male Wistar rats (Taconic) weighing approximately 200 gram are used. Body weight is measured and rats are subsequently anaesthetized with Hypnorm/Dormicum (each compound is separately diluted 1:1 in sterile water and then mixed; prepared freshly on the experimental day). Anaesthesia is initiated by 2 mL/kg Hypnorm/Doricum mixture sc followed by two maintenance doses of 1 mL/kg sc at 30 minutes intervals, and two maintenance doses of 1 mL/kg sc with 45 minutes intervals. If required in order to keep the rats lightly anaesthetised throughout a further dose(s) 1-2 mL/kg sc is supplied. Weighing and initial anaesthesia is performed in the rat holding room in order to avoid stressing the animals by moving them from one room to another.
8
Wistar Rats Longevity Study
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Eight female Wistar rats (Taconic Biosciences, Denmark) were housed in cages in a controlled environment. Caretaking and animal experiment facilities were provided by the Animal Facility, Institute of Biomedicine, Aarhus University, Denmark. Procedures of this study were approved by the Danish Animal Inspectorate (permission no. 2016‐15‐0201‐01057), and their guidelines for use and care of laboratory animals were followed. During the study period, the rats were placed in cages with hamster wheel 30 min twice weekly in an attempt to prolong their life span.
9
Alcohol-Induced Gene Expression in Rats
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A total of 22 male outbred Wistar rats (Taconic, Ejby, Denmark), weighing 160-180g at the start of experiments were single housed during eight weeks, a time period previously reported to be sufficient for the detection of potential alcohol-induced alterations in gene expression [9 (link)]. Animals were housed with a regular 12h light cycle and had ad lib access to tap water and rodent chow (Lantmännen, Stockholm, Sweden). All animals were allowed one week of adaption to the animal facilities before starting experiments. Experiments were carried out in accordance with the guidelines laid down by the Swedish Animal Council and the EU directive 2010/63 regarding the care and use of animals for experimental procedures and were approved by the Ethics Committee for Animal Experiments, Gothenburg, Sweden.
10
Anhedonic and Resilient Rat Brains
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