Lohmann Brown hens’ eggs were a gift from the Tronina factory. Cod liver oil (CLO) was a gift from the National Marine Fisheries Research Institute, Gdynia, Poland. Lipozyme TL IM (a silica granulated Thermomyces lanuginosus lipase preparation, 250 U/g) was a gift from the Novozymes A/S (Bagsvaerd, Denmark). Lipase B from Candida antarctica immobilized in a macroporous acrylic resin (synonym: Novozym 435, >5000 U/g), lipase B from Candida antarctica (CALB, >1800 U/g), lipase A from Candida antarctica (CALA, >500 U/g) both immobilized on resin Immobead 150, lipase from Mucor miehei (Lipozyme®, >30 U/g), lipase from Candida cylindracea (≥2 U/mg) and lipase from Burkholderia cepacia (Amano PS IM, >500 U/g) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A boron trifluoride methanol complex solution (13–15% BF3 × MeOH) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Silica gel-coated aluminum plates (Kieselgel 60 F254, 0.2 mm) used in thin layer chromatography (TLC) and the silica gel (Kieselgel 60, 230–400 mesh) used in the column chromatography were purchased from Merck.
Boron trifluoride methanol complex solution
Manufactured by Merck Group
Sourced in United States
Boron trifluoride methanol complex solution is a chemical compound used in various laboratory applications. It is a clear, colorless liquid that serves as a Lewis acid catalyst. The solution assists in organic synthesis reactions and other chemical processes. Its core function is to facilitate and catalyze specific chemical transformations in controlled laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Immobead 150, by Merck Group (2 mentions)
Lipozyme tl im, by Novozymes (2 mentions)
Lipozyme, by Merck Group (1 mentions)
Supelco 37 component fame mix, by Merck Group (1 mentions)
Milli q water system, by Merck Group (1 mentions)
N hexane, by RCI Labscan (1 mentions)
Novozym 435, by Merck Group (1 mentions)
Model 7890b, by Agilent Technologies (1 mentions)
4 protocols using boron trifluoride methanol complex solution
1
Enzymatic Modification of Cod Liver Oil
2
Fatty Acid Methyl Ester Analysis
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The standard compounds of eicosapentaenoic acid methyl ester, docosahexaenoic acid methyl ester and Supelco® 37 component fame mix were purchased from Sigma-Aldrich (St. Louis, MO, USA). The purity of these chemical standards was more than 98% by GC-MS.
N-hexane was used as a solvent in GC-MS analysis, which was purchased from the RCI Lab-Scan Limited (Bangkok, Thailand). Potassium hydroxide and sodium chloride of analytical grade was purchased from Uni-Chem (Shanghai, China). Boron trifluoride methanol complex solution (13-15% BF3 basis), used to carry out methyl esterification, was purchased from Sigma-Aldrich (St. Louis, MO, USA). Water was purified using a Milli-Q water system (Millipore; Bedford, MA, USA).
N-hexane was used as a solvent in GC-MS analysis, which was purchased from the RCI Lab-Scan Limited (Bangkok, Thailand). Potassium hydroxide and sodium chloride of analytical grade was purchased from Uni-Chem (Shanghai, China). Boron trifluoride methanol complex solution (13-15% BF3 basis), used to carry out methyl esterification, was purchased from Sigma-Aldrich (St. Louis, MO, USA). Water was purified using a Milli-Q water system (Millipore; Bedford, MA, USA).
3
Enzymatic Synthesis of Citronellic Acid
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Lohman Brown hens were a gift from the Tronina factory. Lipase B from Candida antarctica immobilized in a macroporus acrylic resin (synonym: Novozym 435 > 5000 U/g), lipase B from Candida antarctica (CALB > 1800 U/g) and lipase A from Candida antarctica (CALA > 500 U/g) both immobilized on resin Immobead 150 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Immobilized lipase from Rhizomucor miehei (Lipozyme® > 30 U/g) was provided by Fluka (Buchs, Switzerland) while immobilized lipase from Thermomyces lanuginosus (Lipozyme TL IM, 250 U/g) was supplied by Novozymes A/S (Bagsvaered, Denmark). Citronellic acid (CA) (3,7-dimethyl-6-octanoic acid, purity: 98%), a boron trifluoride methanol complex solution (13–15% BF3 × MeOH), sodium methylate and heptane were purchased from Sigma-Aldrich (St. Louis, MO, USA). The thin layer chromatography (TLC) pre-coated silica gel plates (Kieselgel 60 F254, 0.2 mm), the silica gel (Kieselgel 60, 230–400 mesh), solvents used in chromatography and HPLC grade solvents (Merck LiChrosolv® Reag.) were purchased from Merck (Darmstadt, Germany).
4
Proximate Composition and Fatty Acid Analysis
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Proximate composition (moisture, lipid, protein, and ash contents) of the diets and tissue samples were analyzed using the prescribed methods of Li et al. [13 (link)] and Ma et al. [14 (link)]. Fatty acid composition of the diets and tissue samples was determined following the methods detailed by Li et al. [8 (link), 13 (link)]. Briefly, total lipid in each sample was extracted with chloroform/methanol (2 : 1 v/v) and methylated into fatty acid methyl esters (FAME) with KOH-methanol (0.5 M) and boron trifluoride methanol complex solution (15% BF3 basis, Sigma-Aldrich, USA). Then, the FAME were analyzed using a gas chromatograph (GC; Model 7890B, Agilent, USA) equipped with a hydrogen flame ionization detector and a capillary column [8 (link), 13 (link)]. Finally, each fatty acid was identified by comparing the FAME profiles of the samples with known FAME standards (Supelco, 37 Component FAME mix C4-C24, Sigma No. CRM47885, Sigma-Aldrich, USA) and expressed as percentages of total fatty acids [8 (link), 13 (link)].
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