Cells were grown in chamber slides and exposed to non-cytotoxic concentrations of PS (0, 5, 10, 20, 40 μg ml−1) for 24h. Lyso-ID® Red Detection Kit (Enzo Life Sciences, Lausen, Switzerland) was used according to the protocol provided by the producer. Ten μM chloroquine was used as the positive control. After staining of cells with dye for 20 min in the dark, cells were rinsed in Assay Buffer and covered with a coverslip. The slides were scanned in the TissueFAXS (Tissuegnostics, Vienna, Austria) and analyzed by TissueQuest software. Medium-treated cells were used for the gating.
Lyso id red detection kit
Manufactured by Enzo Life Sciences
Sourced in United States
The Lyso-ID® Red Detection Kit is a fluorescent probe designed to detect and visualize lysosomes in live cells. The kit provides a simple, sensitive, and non-cytotoxic method for the labeling and imaging of lysosomes in a variety of cell types.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Dnase 1, by Thermo Fisher Scientific (2 mentions)
Tissuefax, by TissueGnostics (1 mentions)
Tryptone, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Ampicillin, by Merck Group (1 mentions)
Hoechst 33342, by Beyotime (1 mentions)
Tianpure midi plasmid kit, by Tiangen Biotech (1 mentions)
Tcs sp8 confocal microscope, by Leica (1 mentions)
Axio vert a1 microscope, by Zeiss (1 mentions)
Cyto id autophagy detection kit, by Enzo Life Sciences (1 mentions)
4 protocols using lyso id red detection kit
1
Lysosomal Staining of Photosensitizer Exposure
2
Optimized Branched PEI Transfection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Branched PEI (water-free) with molecular weight of 25 kDa, ampicillin, reduced l -glutathione (GSH), MTT, tryptone, and yeast extracts were purchased from Sigma-Aldrich (St Louis, MO, USA). Lipofectamine 2000 (Lipo) reagent, Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), penicillin, streptomycin, DNase I, trypsin, and N,N′-cystamine bisacrylamide (CBA) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). A TianPure Midi Plasmid Kit was purchased from Tiangen Biotech Co Ltd (Beijing, China). Hoechst 33342 and DiO were purchased from Beyotime Institute of Biotechnology (Haimen, China). pSilencer 4.1-CMV FANCF shRNA was kindly provided by the Department of Pharmacology of China Medical University (Shenyang, China). A Lyso ID red detection kit and Total Nuclear ID green/red nucleolar/nuclear detection kit were purchased from Enzo Life Sciences (Farmingdale, NY, USA). FANCF antibody (D2) and goat antimouse IgG fluorescein isothiocyanate were purchased from Santa Cruz Biotechnology Inc (Dallas, TX, USA). Agmatine (Agm) sulfate and l -arginine (Arg) were purchased from Sinopharm Chemical Reagent (Shanghai, China). Acetone, N,N-dimethylformamide, and other common reagents were analytical grade.
3
Autophagy Induction by Ochratoxin A
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Autophagy induction was investigated by examining LC3B-I to LC3B-II conversion by Western blotting and visualizing LC3B puncta formation in stable HK-2 cells expressing GFP-LC3 fusion protein. Stable GFP-LC3 expressing HK-2 cells were treated with 10 µM OTA for 1, 3, 6, 12, and 24 h. As vehicle control, cells were treated with 0.1% v/v Et-OH for 24 h. In order to detect acidic vesicles, Lyso-ID Red Detection Kit (Enzo Life Sciences, Farmingdale, NY, USA) was used as recommended by the manufacturer. Briefly, cells were stained with detection solution; red detection dye for the acidic vesicles, and Hoechst to stain the nuclei. Stained cells were visualized with Leica TCS SP8 confocal microscope (Wetzlar, Germany).
4
Autophagy and Lysosome Imaging in iNPCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The iNPCs were seeded on 18-mm cover-glass bottom dishes and untreated or treated with 500 ng/mL rapamycin for overnight. After the treatments, the cells were stained with a CYTO-ID® autophagy detection kit (Enzo, Farmingdale, NY, USA) and LYSO-ID® Red detection kit (ENZ-51005-0100, Enzo) according to the manufacturer’s instructions. Images were acquired by an Axio VertA.1 microscope (Carl Zeiss).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!