Plasma brain natriuretic peptide (BNP) and Ang II were determined to assess myocardial stretch and renin–angiotensin–aldosterone system (RAAS) system activity, respectively, using available enzyme-linked immunosorbent assay (ELISA) kits (Phoenix Pharmaceuticals, Burlingame, CA, USA). The levels of plasma epinephrine (E) and norepinephrine (NE) were measured to analyze the sympathetic response with commercially available ELISA kits (KA3746, Abnova, Taipei, Taiwan). NE concentrations in the LA, LV, aorta and kidney tissues were also determined (KA3836, Abnova, Taipei, Taiwan). The levels of IL-6, TNF-α and cGMP in the aorta were assessed via available ELISA kits (Abcam, ab-100573, ab-100756 and ab-65356, respectively). Plasma total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), HDL and glycosylated hemoglobin (HbA1c) were measured with specific kits according to the manufacturer’s instructions (Roche Diagnostics, Madison, WI, USA). Urinary electrolyte and osmolality levels were measured by the autoanalyzer CX5 (Beckman Instruments, Fullerton, CA, USA). Urinary glucose and uric acid levels were measured by Cobas Mira autoanalyzer (Roche Diagnostics, Madison, WI, USA).
Ab 100573
Manufactured by Abcam
Sourced in United States
Ab-100573 is a protein-based lab equipment product. It functions as a core component for various laboratory procedures.
Automatically generated - may contain errors
2 protocols using ab 100573
1
Biomarkers of Myocardial Stress and Inflammation
2
Quantifying IL-6 and IL-1β Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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IL-6 and IL-1β levels were measured according to the commercial human IL-6 (ab100573, Abcam, Cambridge, MA, USA) and IL-1β (ab214025, Abcam, Cambridge, MA, USA) ELISA kit. Blank, standard, and sample wells were set up according to the manufacturer's instructions, with 3 replicates per well. After washing with PBS, 100 μL of enzyme-labeled secondary antibody was added and incubated for 60 min at 37 °C. After washing the unbound antibody, the substrate was added and incubated for 20 min at room temperature before adding the termination solution and detecting the OD450. The expression levels of IL-6 and IL-1β were calculated.
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