Four-week-old male C57BL/6 mice (n = 12) and ApoE–/– mice (apolipoprotein E knockout mice in C57BL/6 background; n = 12) were purchased from Model Animal Research Center of Nanjing University, Nanjing, China. Mice were maintained in specific pathogen-free conditions with a regular diet and cared for according to the ethics committees of Nanjing University; this study was conducted in accordance with the standards of the Helsinki Declaration.
C57bl 6 mice
Manufactured by Model Animal Research Center of Nanjing University
C57BL/6 mice are a widely used inbred strain of laboratory mice. They are a common model organism for biomedical research due to their well-characterized genetics and physiology.
Automatically generated - may contain errors
Lab products found in correlation
Mip 2, by R&D Systems (2 mentions)
Mip 1α, by R&D Systems (2 mentions)
Apoe mice, by Model Animal Research Center of Nanjing University (1 mentions)
Puromycin, by Cayman Chemical (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Gw9662, by Cayman Chemical (1 mentions)
Rosiglitazone rosi, by Cayman Chemical (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
Raw264.7 cell, by American Type Culture Collection (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (1 mentions)
Secondary horseradish peroxidase hrp conjugated antibodies, by Jackson ImmunoResearch (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Thp 1 cell, by American Type Culture Collection (1 mentions)
Mh s cells, by American Type Culture Collection (1 mentions)
26 protocols using c57bl 6 mice
1
Apolipoprotein E Knockout Mice Study
2
Septic AKI Model in Mice via CLP
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For this study, 8–10-week-old C57BL/6 mice (male, pathogen-free) were purchased from the Model Animal Research Center of Nanjing University (Nanjing, China). Consistent with previous reports (Rittirsch et al., 2009 (link)), a septic AKI animal model was built by cecal ligation and puncture (CLP) surgery. Mice were anesthetized with 2% isoflurane induction and 1.4% isoflurane maintenance, the cecum was silk-ligated and pierced twice by a 20-gauge needle, and after closing the abdomen, saline was injected subcutaneously for fluid resuscitation. Blood and kidney samples were harvested after 24 h. A 20 mg/kg/d dose of DHT was injected intraperitoneally into the mice for three consecutive days prior to CLP surgery. The sham group received identical dissection compared to the mice in the CLP group but without cecum ligation and puncture.
3
Gut Microbiome Modulation in Endotoxemia
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C57BL/6 mice (20 ± 2 g, 6-8 weeks old) were purchased from Model Animal Research Center of Nanjing University (Nanjing, China). Animal care was performed in accordance with guidelines of the Zhejiang Ethics Committee and received prior approval from the Animal Care and Use Committee in Zhejiang A & F University. Mice were kept at a constant temperature of 26 ± 2 °C with 12 h light–dark cycles and provided with ad libitum feed intake and water. The mice were randomly assigned to four groups (n= 6): two of them, designed as control and LPS groups, received PBS. The others, named as LGG + LPS and ZJ617 + LPS respectively, orally inoculated with LGG or ZJ617 suspended in sterile PBS at the concentration of 1×108 CFU/ml daily prepared respectively. At 7 days after starting the oral administration of the strain, LPS stimulation was conducted in mouse from LPS, LGG + LPS and ZJ617 + LPS groups with an intraperitoneal (i.p.) injection of 10mg/kg LPS respectively. The control groups received an i.p. injection of sterile PBS. The used does of LPS did not induce mice death in the following 24 h determined by our previous study [19 (link)]. Blood samples and ileum tissues (5 cm proximal from the ileal-cecal junction) were collected 24 h after the LPS challenge.
4
Murine Xenograft Model of Colorectal Cancer
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A total of 40 (20 experimental + 100% extra as spare) female C57BL/6 mice were purchased from Model Animal Research Center of Nanjing University (Nanjing, China). An acclimation period of ~1 week was allowed before tumor inoculation. Mice were kept in a special pathogen-free environment in microisolator cages at constant temperature (20-26°C), constant humidity (40-70%) and on a 12 h light/dark cycle. Animals had free access to food and water throughout the study. At the time of MC38 cell inoculation, the animals were 7–8 weeks of age and weighed 18–22 g. Before implantation, mice were lightly anesthetized via 1–4% isoflurane inhalation. Each mouse was inoculated subcutaneously into the right lower flank with 1×106 single cells of ≥95% viability suspended in 0.1 ml of DMEM (without serum and without phenol red).
5
Murine Model for Pathogen-Free Research
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Six to eight weeks male C57BL/6 mice were purchased from Model Animal Research Center of Nanjing University. Mice were bred under specific pathogen-free condition and received a 12 h light : 12 h dark cycle. All animal experiments were approved by the institutional review committee of the Sun Yat-sen University and performed in strict compliance with the national and institutional guidelines.
6
Generating F1 and F2 Mouse Populations
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A/J and C57BL/6 mice were purchased from Model Animal Research Center of Nanjing University (Nanjing, China). A/J male mice were mated with C57BL/6 female mice to generate an F1 population. For generation of F2 progeny, F1 male mice were mated with inbred F1 female mice (Additional file 1 : Figure S1). All the mice were raised to 8–10 weeks old in the Laboratory Animal Center of Nanjing Agricultural University (Nanjing, China), and were allowed to acclimate for more than 7 days with free access to rodent chow and water before experiments.
7
Murine Model of Inflammatory Response
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Pathogen-free male C57BL/6 mice that are 8–12 weeks old are obtained from Model Animal Research Center of Nanjing University (Nanjing, China), and all animal experiments are performed according to the protocol approved by Southeast University. RAW264.7 cells and HEK293 cells are purchased from American Type Culture Collection and cultured in DMEM supplemented with 10% fetal bovine serum (Gibico). Bovine serum albumin (BSA) and rabbit anti-BSA IgG (α-BSA) are purchased from Invitrogen and MP Biomedicals, respectively. ELISA kit for mouse albumin is purchased from Bethyl Laboratories. ELISA kits for TNF-α, MCP-1, MIP-1α and MIP-2 are purchased from R&D Systems. PPARγ antagonist GW9662, PPARγ agonist Rosiglitazone (ROSI) and puromycin are purchased from Cayman Chemical.
8
Murine Model of Schistosoma japonicum Infection
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Specific pathogen-free (SPF) 8-wk-old female C57BL/6 mice were purchased from the Model Animal Research Center of Nanjing University (Nanjing, China). All the mice were housed and handled in accordance with the guidelines of Chinese animal protection laws with permission from the Institutional Review Board of Nanjing Medical University.
Each C57BL/6 mouse was percutaneously infected with 12 cercariae of the Chinese mainland strain of S. japonicum from infected snails (Oncomelania hupensis) acquired from the Jiangsu Institute of Parasitic Diseases (Wuxi, China).
Each C57BL/6 mouse was percutaneously infected with 12 cercariae of the Chinese mainland strain of S. japonicum from infected snails (Oncomelania hupensis) acquired from the Jiangsu Institute of Parasitic Diseases (Wuxi, China).
9
Euthanasia Protocol for Mouse Tissue Harvesting
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Adult female C57BL/6 mice (weight, 17–20 g; age, 6–8 weeks; n=3) were purchased from the Model Animal Research Center of Nanjing University and maintained under standard animal housing conditions (12-h light/dark cycle and free access to food and water) in a temperature-controlled room (24±1°C) with relative humidity (50±10%) at the animal center of Nanjing Medical University. All procedures involving mice and the corresponding experimental protocols were approved by the Animal Care and Use Committee of Nanjing Medical University (approval no. IACUC-1706005). Following terminal anesthesia by intraperitoneal injection with pentobarbital sodium (100 mg/kg body weight), mice were euthanized by cervical dislocation and the death of mouse was verified 10 min by the following criteria: i) No breathing; ii) no nerve reflexes; iii) no heartbeat and iv) relaxed muscles. Then, the tissues (including the heart, liver, spleen, lung, kidney, bone and brain) were quickly dissected and separately immersed into liquid nitrogen.
10
Conditional Knockout of Hdac2 in Mice
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HDAC2flox/flox mice (C57/BL6 background), exons 5 and 6 with loxP recombination sites, were generated and maintained at Model Animal Research Center of Nanjing University (Nanjing, China). A total of 38 male HDAC2flox/flox mice were used in this study. Nervous system Hdac2 conditional knockout (Hdac2 CKO) mice were generated by crossing Nestin‐CRE+/− mice with HDAC2flox/flox mice (from Model Animal Research Center of Nanjing University, China). A total of 40 male Hdac2 CKO mice were used in experiments. A total of 492 male young adult (6–7 weeks) C57BL/6 mice (from Model Animal Research Center of Nanjing University, China) were used. An experimenter labeled all animals before allocation. Experiments were performed by investigators who were blinded to group allocation. All animal protocols were approved by the Institutional Animal Care and Use Committee of Nanjing Medical University.
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