Monoclonal rat

Rhodamine–phalloidin (for actin staining) was from Invitrogen (Carlsbad, CA); anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH), anti-HA, phospho-paxillin (Y118), monoclonal anti-Cdc42 antibodies, anti-GFP monoclonal antibody, active 9EG7 and total Hmβ1-1 CD29 β1-integrin, and monoclonal paxillin antibodies were from BD Biosciences (Palo Alto, CA); rat immunoglobulin G (IgG) a,κ and hamster IgG isotype controls were from Biolegend (San Diego, CA). Monoclonal rat from Roche Molecular Biochemicals (Indianapolis, IN) or monoclonal rabbit (from Cell Signaling Technology, Beverly, MA) anti-HA antibody was used for cell staining; antibodies against mouse FAK, phospho-FAK (Y397), vinculin, and α-tubulin were from Abcam (Cambridge, MA). Mouse monoclonal pan-arrestin F4C1 antibody recognizing epitope DGVVLVD in the N-domain was a generous gift of L. A. Donoso (Wills Eye Hospital, Philadelphia, PA). Arrestins were detected with arrestin-2-specific (1:6000; Mundell et al., 1999 (link)) or arrestin-3-specific (1:700; Orsini and Benovic, 1998 (link)) affinity-purified rabbit polyclonal antibodies. Total integrin antibody M-106 for Western blot was from Santa Cruz Biotechnology (Santa Cruz, CA).