Poly diallyldimethylammonium chloride pdda

Poly (diallyldimethylammonium chloride) (PDDA) with a molecular weight of Mw = 450,000 and 40 wt% in H₂O was obtained from Sigma-Aldrich (Germany). The raw multifloral Persian Honey was obtained from a local company at Polour, Iran. Glutaraldehyde (GA) (50% V/V in water) and aluminum trichloride (AlCl₃) were obtained from Merck (Germany). Ethanol, nutrient broth solution, phosphate-buffered saline, Folin–Ciocalteu’s phenol, Sodium carbonate (Na₂CO₃), mEthanol, and sodium citrate were of analytical grade and obtained from Merck Millipore (Germany). Sterile streptozotocin (STZ), xylazine, and ketamine were received from Sigma, St Louis, MO, USA.

Bulk In₂Se₃ was purchased from Six Carbon Technology (Shenzhen, China). Tetraheptylammonium bromide (THAB) was purchased from Aladdin (Shanghai, China) Polyvinyl pyrrolidone (PVP) was purchased from Energy Chemical (Shanghai, China). Poly(diallyldimethylammonium chloride) (PDDA) was purchased from Sigma-Aldrich (Shanghai, China). Polyurethane was purchased from Yantai Wanhua Polyurethane Co., Ltd. (Yantai, China). Cr and Au metals were purchased from Vnano Vacuum Technology Co., Ltd. (Beijing, China). Carbon rod was purchased from Tianjin Aida Hengsheng Technology Development Co., Ltd. (Tianjin, China). Other solutions, including Acetonitrile, N,N–Dimethylformamide (DMF), acetone, and ethanol and isopropyl alcohol were purchased from Tianjin Chemical Reagent Company (Tianjin, China). Ultrapure water (20 MΩ. cm⁻¹) was prepared by a Sartorius Arium pro UF system made by sartorius (Göttingen, Germany).

Silver nitrate (99.9%, Aldrich), ammonia (25% (w/w) aqueous solution (p.a. Lach-Ner), sodium hydroxide (p.a. Lach-Ner), d-maltose monohydrate (p.a. Ridel-de-Haen), ascorbic acid (p.a. Penta), and sodium borohydride (p.a. Sigma-Aldrich) were used without purification for the preparation of AgNPs. The Tollens process was further modified by the presence of a natural polymer: gelatin (p.a. Loba Feinchemie). The aggregation study was performed with poly(diallyldimethylammonium) chloride (PDDA) (p.a. Sigma-Aldrich). All of the solutions used were prepared using demineralized water (conductivity 0.05 µS·cm^–1) from an Aqual 29 water purification system (Merci).

2,4-Dibromophenyl 2-bromophenyl ether (BDE17), 2,4,4′-tribromodiphenyl ether (BDE28), 2,3′,4′,6-tetrabromodiphenyl ether (BDE 71), 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47), 2,3′,4,4′-tetrabromodiphenyl ether (BDE 66) and 2,2′,4,4′,6-pentabromodiphenyl ether (BDE 100) were obtained from Accu Standard Inc. (New Haven, CT, USA). Ct-DNA (Lot: D4522-1MG, Sigma, New York, NY, USA) and poly(diallyldimethyl ammonium chloride) (PDDA, 20%, w/w, in water, MW = 100,000–200,000) were purchased from Sigma-Aldrich (New York, NY, USA). The carboxyl of graphenes (purity > 95 wt%, ASH < 1.5 wt%, SSA > 500 m²/g) were purchased from Nanoport. Co. Ltd. (Shenzhen, China). Ferric chloride (FeCl₃·6H₂O), ammonia (28%), n-hexane, ethylene glycol, tetraethyl orthosilicate (TEOS), sodium acetate and dichloromethane were purchased from Beijing Chemicals Corporation (Beijing, China). The syringe filters were purchased from Xingya (Shanghai, China). All other chemicals were used as received without further purification. The water in this work was deionized water.

All solutions were prepared with distilled/deionized water (18 MΩ resistivity, Darmstadt, Germany). Carbon nanotubes were obtained from NANOCYL®NC7000(Austin, TX, USA). Nitric acid (HNO₃, 69.2 wt%) and hydrogen peroxide ( ${\mathrm{H}}_2{\mathrm{O}}_2$ 30% v/v) were purchased from Sigma-Aldrich (Darmstadt, Germany). Potassium phosphate monobasic ( $\mathrm{K}{\mathrm{H}}_2\mathrm{P}{\mathrm{O}}_4$ ) and sodium hydroxide (NaOH, 99.9% p/p) were purchased from Fisher Scientific (Waltham, MA, USA). Phosphate-buffered saline (PBS: $\mathrm{K}{\mathrm{H}}_2\mathrm{P}{\mathrm{O}}_4$ + ${\mathrm{K}}_2\mathrm{HP}{\mathrm{O}}_4$ + KCl, at different pH values) was used as a supporting electrolyte. Potassium ferricyanide ( ${\mathrm{K}}_3\left[\mathrm{Fe}{\left(\mathrm{CN}\right)}_6\right]$ ), iron trichloride hexahydrated ( $\mathrm{FeC}{\mathrm{I}}_3\cdot 6{\mathrm{H}}_2\mathrm{O}$ ), and potassium chloride (KCl) were obtained from BDH Chemicals (Philadelphia, PA, USA); hydrochloric acid (HCl, 37%) was from Fisher Scientific (Waltham, MA, USA); glassy carbon (GC, $\Phi =3\mathrm{mm}$ , geometric area = 0.0706 cm²), silver/silver chloride reference electrode (Ag/AgCl), and graphite rod counter electrode were from CH Instruments (Austin, TX, USA); sulfuric acid ( ${\mathrm{H}}_2\mathrm{S}{\mathrm{O}}_4$ , 98%) was from Fisher Scientific (Waltham, MA, USA); 1 μm, 0.3 µm, and 0.05 µm alumina powder were from CH Instruments (Austin, TX, USA); dimethylformamide (DMF) was from BDH Chemicals (Philadelphia, PA, USA); and poly (diallyldimethylammonium chloride) (PDDA, 4% w/w in water) was from Sigma.

Tetraethylorthosilicate (TEOS), absolute ethanol, Hexamethyldisilazane (HDMS) and ammonia (25%) were received from Sinopharm Chemical Reagent. Aniline monomer (ANI), ammonium persulphate (APS), N-methyl-2-pyrrolidone (NMP) and hydrochloric acid (HCl) were purchased from Shanghai Lingfeng Chemicals. Polyacrylic acid (PAA) of average Mw ~ 450,000 and polydiallyldimethylammonium chloride (PDDA) of average Mw ∼ 200,000–350,000 were purchased from Sigma-Aldrich. The 316SS coupons (2.5 × 2.5 × 0.1 cm) were supplied by Senda decoration materials (China). The coupons were polished with different grades of SiC papers up to 1200 and then the surface was polished with 3μm alumina slurry. Prior to coating, the coupons were washed in deionized water and dried.

Tyramine (99 %), glutathione (99 %), phenylalanine (98 %), titanium(IV) isopropoxide, tyrosinase from mushroom (EC1.14.18.1; 5771 U mg⁻¹), vanillic acid, gallic acid, and poly(diallyldimethylammonium chloride) PDDA (average Mw < 100,000, 35 wt% in H₂O) were purchased from Sigma-Aldrich (USA); tryptophan (98.5–101 %) and catechol were from Merck (Germany) and caffeic acid was from Koch-Light Laboratories; HCl (35 %), ethanol (96 %), 2-propanol, and l-(+)-ascorbic acid were obtained from Avantor Performance Materials Poland S.A. (Poland); HNO₃ (65 %), NH₃(aq) (25 %), and acetone were purchased from LACHNER (Czech Republic); 0.3 mm alumina powder was from Buehler Micropolish (USA); Nafion (5 % (w/v) solution in mixture of low aliphatic alcohol and water) was obtained from Fluka. Furfuryl alcohol (98 %, Aldrich), hydrochloric acid (33 %, Polish Chemical Reagents), and iron(III) nitrate nonahydrate (Polish Chemical Reagents) were used for CMK-3 synthesis without any further purification. Phosphate buffer solutions (PBS) of pH 6.0 and 7.0, in a concentration of 0.1 M, were prepared by mixing appropriate volumes of KH₂PO₄ and Na₂HPO₄ solutions. Tyrosinase (0.048 mg/10 μL) and Tyramine solution were prepared in 0.1 M phosphate buffer solution, pH 7. All chemicals were analytical-grade reagents. Milli-Q water was used throughout.