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Irdye800 conjugated streptavidin

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IRDye800 conjugated streptavidin is a fluorescent labeling reagent. It consists of the protein streptavidin conjugated to the near-infrared dye IRDye800. The core function of this product is to provide a way to detect and visualize biotinylated molecules through fluorescence imaging.

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Lab products found in correlation

Alexa fluor 680 goat anti mouse, by Thermo Fisher Scientific (2 mentions) Goat anti mouse cy2, by Jackson ImmunoResearch (1 mentions) Mouse anti gfp jl 8, by Takara Bio (1 mentions) Rabbit anti phospho histone h3 ser10, by Merck Group (1 mentions) Digitonin, by Thermo Fisher Scientific (1 mentions) Slowfade gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Mouse anti gfp, by Takara Bio (1 mentions) Cy2 affinipure donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Palmitic acid alkyne, by Cayman Chemical (1 mentions) Immobilon fl, by Merck Group (1 mentions) Odyssey blocking buffer in pbs, by LI COR (1 mentions) Pcdna3, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Fugene hd, by Promega (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Trypsin, by Corning (1 mentions) Ethylene diamine tetraacetic acid (edta), by Corning (1 mentions) Mouse anti pcna, by Santa Cruz Biotechnology (1 mentions) Mouse anti β tubulin, by Santa Cruz Biotechnology (1 mentions) Anti flotillin 1, by BD (1 mentions)

Spelling variants of this product

Streptavidin-IRDye800 (5 citations)

3 protocols using irdye800 conjugated streptavidin

1

Antibody Characterization for Wnt1 and GFP Detection

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Mouse anti-chick WNT1 5F1-G11-D1 was used for western blots (1/10) while mouse anti-chick WNT1 7B3-A10-F9 was used for immunoprecipitations (1/4). Both antibodies were made in the Burrus lab (Galli et al., 2007). Mouse anti-GFP JL-8 (Clontech) (immunoprecipitation 1/750, colorimetric Westernblot 1/1500, fluorescent western blot 1/15000); Rabbit anti-Phospho Histone H3 (ser10) (Millipore) (1:1,000 dilution); IRDye800 conjugated streptavidin (Licor) (1/5000); Alexa Fluor® 680 Goat Anti-Mouse (Invitrogen) (1/4000); Goat anti mouse Cy2, Goat anti mouse Cy3, Goat anti-Rabbit DyLight649 (Jackson Immunoresearch Labs) (1/200); mouse anti-Islet1 conditioned media (Developmental Studies Hybridoma Bank) (1:20).
Miranda M., Galli L.M., Enriquez M., Szabo L.A., Gao X., Hannoush R.N, & Burrus L.W. (2014). Identification of the WNT1 residues required for palmitoylation by Porcupine. FEBS letters, 588(24), 4815-4824.
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2

Characterization of Protein Lipidation

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Materials and their respective vendors are as follows: DMEM, PBS, 200 mM l-Glutamine, 100× Penicillin/Streptomycin 0.25% Trypsin + 0.1%EDTA (Corning); Fetal Bovine Serum (Atlanta Biologicals); Fugene HD (Promega); Triton-X 100, DAPI (Roche); digitonin (ACROS); HEK 293T/17, COS-7, mouse anti-MYC 9E10.2 (conditioned media used at 1/10 dilution) (ATCC); 8-chamber glass slides, mouse anti-HA (1/2000 dilution) Immobilon-FL (Millipore), 16% paraformaldehyde (EMS); BSA, rabbit anti-FLAG (1/2000 dilution), DMSO, fatty acid free BSA, TCEP Tris(2-carboxyethyl) phosphine hydrochloride, TBTA Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine, Copper Sulfate, NP-40 (Sigma); Alexa Fluor 647-AffiniPure Goat Anti-rabbit IgG (H + L) (1/200 dilution), Cy2 AffiniPure Donkey Anti-Mouse IgG (H + L) (1/200 dilution), anti-mouse Alkaline Phosphatase (H + L) (1/1500), anti-rabbit Alkaline Phosphatase (H + L) (1/1500) (Jackson ImmunoResearch); Mouse anti-GFP (1/15 000 dilution) (Clontech); SlowFade Gold antifade reagent (Thermo Scientific); Biotin Azide, Alexa Fluor_680 Goat Anti-mouse (1/4000 dilution), pcDNA3.1 (Invitrogen); IRDye800 conjugated streptavidin (1/5000 dilution), Odyssey blocking buffer in PBS (Licor); Protein A/G agarose beads, Halt protease and phosphatase inhibitor cocktail (Pierce); and Palmitic Acid Alkyne (Cayman Chemical).
Galli L.M., Anderson M.O., Gabriel Fraley J., Sanchez L., Bueno R., Hernandez D.N., Maddox E.U., Lingappa V.R, & Burrus L.W. (2021). Determination of the membrane topology of PORCN, an O-acyl transferase that modifies Wnt signalling proteins. Open Biology, 11(6), 200400.
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3

Immunofluorescence Antibody Staining Protocol

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Mouse anti-PCNA (1:1000), mouse anti-β-Tubulin (1:400) (Santa Cruz Biotechnology); mouse anti-chick Wnt1 5F1-G11-D1 was made in the Burrus lab (1:10); rabbit anti-human PORCN was made in collaboration with Prosetta Biosciences (1/5000); anti-mouse Alkaline Phosphatase (1:1500), anti-rabbit Alkaline Phosphatase (1/1500) anti-mouse Dylight 649 (1/200)(Jackson Immunoresearch); anti-Flotillin-1 (1:1500) (BD Biosciences); Alexa Fluor® 680 Goat Anti-Mouse (1/4000) (Invitrogen); IRDye800 conjugated streptavidin (1/5000) (Licor); mouse anti-HA (1/1000) (Upstate, Millipore); mouse anti-H6 (1/500) (Roche); mouse anti-MYC (1/10) (conditioned media made from cell line from ATCC CRL-1729).
Galli L.M., Zebarjadi N., Li L., Lingappa V.R, & Burrus L.W. (2016). Divergent effects of Porcupine and Wntless on WNT1 trafficking, secretion, and signaling. Experimental cell research, 347(1), 171-183.
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