Thioflavin T (ThT) fluorescence spectra were recorded on Fluorolog-3 Model FL3-22 spectrofluorometer (Horiba Jobin Yvon, Park Avenue Edison, NJ, USA). ThT spectra of samples were recorded in 10 µM ThT solution in 10 mM phosphate buffer pH 7.4. Briefly, peptide solutions were diluted to 10 µM peptide concentrations in 10 µM ThT solution in 10 mM phosphate buffer pH 7.4. Blank ThT spectra were recorded by adding same volumes of cosolvents in 10 µM ThT solution in 10 mM phosphate buffer pH 7.4. The excitation wavelength was set at 450 nm, slit width at 2 nm, and emission slit width at 5 nm.
Fluorolog 3 model fl3 22 spectrofluorometer
Manufactured by Horiba
Sourced in United States
The Fluorolog-3 Model FL3-22 is a spectrofluorometer manufactured by Horiba. It is designed to measure the fluorescence emission spectra of samples. The instrument is capable of recording fluorescence excitation and emission spectra, as well as time-resolved fluorescence measurements.
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2 protocols using fluorolog 3 model fl3 22 spectrofluorometer
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Thioflavin T Fluorescence Spectroscopy
2
Fluorescence Spectroscopy of Nanoparticles
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Steady-state fluorescence spectra for the nanoparticle suspensions were recorded on a Horiba Fluorolog-3 Model FL3-22 spectrofluorometer (double-grating excitation and double-grating emission monochromator) after excitation. Optically dilute aqueous solutions of the nanoparticles, with absorbance < 0.1 au, were prepared in 1 cm path length quartz cuvettes. Fluorescence spectra were obtained under ambient conditions (i.e., air, ~ 21% oxygen in volume). For phosphorescence measurements, vials were capped with a 12 mm PTFE/silicone/PTFE seal (Chromatography Research Supplies) and were continuously purged with analytical grade N2 (Praxair) for 5 min before measurements. For 21% O2 (i.e. air), measurements were taken under ambient conditions (open vial, no cap). Oxygen calibration was performed as previously described with Cole-Palmer flow gauges with mixtures of analytical grade O2, N2, and 1% O2 (in N2) gases (Praxair)29 (link).
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